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Features at a glance
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Sample volumes from 10 µl to 10 ml processable
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High speed, 15 min. for 96 low volume blood samples
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Low to high throughput (4,000 samples/d)
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96 Rod Head for parallel separating and resuspending
in 96 well plates
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12 Rod Head for parallel separating and resuspending
in 15 ml or 50 ml Falcon tubes
Automated High Throughput Nucleic Acid Isolation Based on Proprietary M-PVA Magnetic Bead Technology.
In cooperation with the Forschungszentrum Karlsruhe GmbH, chemagen has developed an innovative robot to realize high throughput sample preparation based on its M-PVA Magnetic Beads.
The chemagic Magnetic Separation Module I is the complete solution for preparation of nucleic acids from various sample material, such as blood, tissue, bacteria, food, plants, etc. Due to it's flexibility it can be applied e.g. for PCR-product purification, Genotyping, Virus Detection, Forensic, GMO-testing and HLA-typing.
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Why use the chemagic Magnetic Separation Module I
Classical methods of DNA and RNA isolation are based on column or precipitation methods. These techniques require centrifugation or vacuum steps and often have lengthy processing times as well as volume limitations, complicating their integration into automated high throughput processes. Additionally, the automated processing of these methods may cause cross contamination of the purified nucleic acids making this method useless for many diagnostic purposes.
Another problem applying automated magnetic bead based separation technologies is the insufficient or ineffective resuspension of the magnetic bead pellets after magnetic separation to enable sufficient washing and elution steps. This ineffectiveness results in lower yields and purity of the isolated nucleic acids.
The chemagic MSM I uses a unique technology to avoid these emerging problems. In the chemagic system, magnetic separation is achieved through the use of an electromagnet and separation heads with magnetizeable rods. The metal rods are immersed into the magnetic bead suspension. When the electromagnet is switched on, the rods become magnetic and the beads are separated. To resuspend an once separated magnetic bead pellet, the magnet is switched off and a stirring motor to which all rods are connected is switched on. This leads to a very effective and gentle resuspension process resulting in separation products with both highest yields and purities (see animation).
Obstacles and Solutions at a Glance
While screening the existing technologies chemagen identified and solved the following obstacles of automated nucleic acid separation:
- Restricted working volumes
chemagen: fully scalable
- Limited to special reaction vessels
chemagen: all standard vessels
- High throughput applications cannot be realized
chemagen: up to 4,000 samples/d
- Additional high costs of disposables
chemagen: transparent costs
In most cases sample preparation is the bottleneck in HLA registry procedure. While diagnostic methods like SSO, SSP and SBT can be carried out simeltanously and cover a high sample throughput, commonly used DNA separation methods require hands on time and often don't produce reliable and constant results. The chemagic Magnetic Separation Module I bridges this gap delivering high yield und quality DNA - fast and fully traceable. |
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The downstream applications utilized in human genetic studies have to rely upon quality and stability of the purified DNA. Besides, while detecting mutations often high volume samples are needed to isolate a sufficient amount of DNA for the following detection reactions. The chemagic MSM I is a perfect solution to this challenge. The quality and robustness of the purified DNA is proven in downstream applications such as SSCP (Fluorescent, Phast System), WAVE dHPLC and fragment analysis. |
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Whether for virus and pathogen diagnostics or for genetic studies - the need for automated isolation of nucleic acids is ubiquitous in the field of veterinary medicine. Due to the diversity of sample material and nucleic acids there are several requirements an isolation system has to fullfill. The Magnetic Separation Module I meets these high demands. The exchangeable heads lead to high flexibility dealing with different sample volumes and chemagen's unique separation technology faciliates the processing of even viscous sample material through it's rotatable and magnetisable rods. Together with the huge variety of chemagic Kits it provides you with the optimal solution for your sample preparation |
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 Hochdurchsatzanalyse der Scrapie-Anfälligkeit bei Schafen
Application Note: Viral Testing at the FLI
The chemagic Magnetic Separation Module I can be used with exchangeable heads which allow the processing in 96- or 48-well-plates using a head with 96 magnetizable rods, in 24-well-plates using a head with 24 rods or in larger tubes (working volumes up to 50 ml) using the chemagic 12 Rod Head. The resulting flexibility makes it possible to meet individual requirements for special applications. Apart from its use as a stand-alone instrument, the cheamgic MSM I can be combined with standard liquid handling units ( More info). |
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chemagic Head |
96 magnetizable rods |
24 magnetizable rods |
12 magnetizable rods |
Formats |
96-, 48- or 24-well plate |
24-well-plates or24 single tubes |
12 tubes (e.g. 50 ml) |
Throughput |
e.g. 4,000 samples/d |
e.g. 288 samples/d |
e.g. 144 samples/d |
Sample volume |
e.g. 10 µl - 1.5 ml blood |
e.g. 200 µl - 2.0 ml blood |
e.g. 0.5 ml - 10 ml blood |
Processing time |
< 15 min for 96 blood samples |
< 45 min for 24 blood samples |
< 45 min for 12 large blood samples |
Power supply |
90 - 240 V; 16 A; (50/60 Hz)
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Dimensions [mm] |
Base machine: 600 x 640 x 950 (W/D/H), Carrier axis: 1940 x 170 x 200 (W/D/H)
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Accessories
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Reduced hands-on time applying this system developed for parallel dispensing even of large volumes.
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Software fullfilling the requirements of quality assurance procedures
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chemagic Dispenser
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General
The use of the chemagic MSM I simplifies and fastens time consuming sample preparation steps like nucleic acid isolation significantly. By using the chemagic Dispenser it is even faster and more comfortable.
The chemagic Dispenser was developed to notably reduce hands-on time preparing a chemagic MSM I run – especially when larger volumes are required. But independent of the amount of needed buffer this particular add-on prefills fast and accurately the tubes/plates during a chemagic MSM I run.
This precise technology of the chemagic Dispenser is also integrated in the chemagic Prepito. |
Features at a glance
- Reduced hands-on time
- Simultaneous filling of up to 8 different buffers with different buffer volumes
- Waste of buffer is minimized
- Based on membrane pump technology
- Fully integrated in the chemagic MSM I software
- Easy upgrade of a stand-alone chemagic MSM I (plug and play
The following example gives an overview of the manual and automated steps using the
stand-alone chemagic MSM I with a chemagic Dispenser.
Manual procedures |
Automated procedures |
One rack with disposable tips and four racks with empty 50 ml tubes (4, 8 or 12 tubes) are placed on the tracking unit |
Isolation protocol is started, membrane pumps are activated |
Blood and protease (if needed) is filled into empty 50 ml tubes |
Lysis buffer is added via the chemagic Dispenser and blood/lysis buffer solution is transported under the head and stirred |
Beads are filled into empty 50 ml tubes |
After lysis has been completed (e.g. 10 min), racks are transported back to appropriate position for further liquid handling |
Racks are placed on the tracking unit |
Binding buffer is added to blood lysate and wash buffers are added to empty tubes |
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Pumps stop |
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Tracking unit moves plate with beads (in wash buffer 5) under the head, beads are picked up and stirred into binding buffer/blood lysate |
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Rest of the isolation protocol is processed |
The first three points of the manual procedures can be avoided using a liquid handling robot. The empty racks are then positioned on the robot platform.
chemagic Dispenser for chemagic 12, 24 and 96 Rod Head
Different versions of the chemagic Dispenser are available corresponding to the three chemagic Rod Heads. See table for details.
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| chemagic Dispenser |
chemagic Rod Head |
Tubes/Plates |
Application example |
chemagic 12 Manifold 12
article No. 525 |
chemagic 12 Rod Head
article No. 507 |
50 ml tubes |
chemagic DNA Blood Kit special for 10 ml blood
article No. 704 |
chemagic Manifold 24
article No. 526 |
chemagic 24 Rod Head
article No. 508 |
24-well plate |
chemagic DNA Blood Kit special for 1,8 ml blood
article No. 1050 |
chemagic Manifold 96
article No. 527 |
chemagic 96 Rod Head
article No. 509 |
96-well plate |
chemagic DNA Blood Kit special for 250 µl blood
article No. 746 |
chemagic QA Software
Features at a Glance
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Barcode reading/Sample tracking
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LIMS compatible log files
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Up to date user management
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Easy navigation and set up
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Pre installed protocols
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Tracking the progress of a running protocol
The chemagic QA Software is the ideal solution for labs who require high quality assurance while they use the chemagic Magnetic Seperation Module I for nucleic acid isolation. The following screen shots show some of the features.
Main Screen Protocol Selection
The main screen shows an overview of the main functions and status of the instrument. The 4 main parts of the machine provide information on:
- selection of pre-installed protocols
- status of the processing protocols
- control button
- instrument status
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ID Window
Within the "ID" Window the primary sample ID´s can be registered manually or by scanning the barcoded primary tubes. Another or the identical code can be assigned to the corresponding elution tube. The upper part of the window shows schematically the x-axis of the instrument with an allocation of each kit componentto be used at its specific position. Identification of each Individual rack creates a further option. |
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Status Protocol
The status protocol shows within the main window: the running time, remaining time and the actual protocol step while running the protocol enabling the complete tracking of the process |
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Log File Window
The log file window enables the access to all log files. The files contain all the information needed for the complete documentation of the isolation process and sample tracking. The files tamper proof/right protected and can be printed out at anytime. LIMS compatibillity is guarranteed for all systems which except plain text files. |
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chemagic QA Software upgrade 5.1 available
The chemagic QA Software upgrade 5.1 is now available and promises a more convenient and flexible handling of the chemagic MSM I with/without chemagic Dispenser. It is compatible with all three chemagic Rod Heads (12, 24 and 96).
The main improvements of this particular upgrade
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Controlling of all three chemagic Rod Head formats (12, 24 and 96)
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Dispensing in the 6 x 4 format corresponding to the chemagic 24 Rod Head is implemented
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Different dispensing formats can be used with chemagen´s chemagic Dispenser
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Selection of individual columns for dispensing is possible to save disposables
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Service protocols are integrated to facilitate a convenient handling
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Cleaning protocols are integrated to fasten the maintenance
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Remaining time to the external step is shown to facilitate the laboratory workflow
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Text files containing sample IDs/scanned bar codes can be integrated (as a txt-file) in the log file to avoid sample mix-up caused by manual enter
For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.
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