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chemagic DNA Blood Kits
chemagen offers a huge variety of nucleic acid isolation kits from whole blood. To facilate your search we differentiate on our internet pages between low and large volume applications. Please choose your required volume range.
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Low Volume (1 µl - 600 µl) DNA Isolation from Blood |
>> General Information
Manual Handling
The chemagic DNA Blood10 Kit and the chemagic DNA Blood100 Kit provide an efficient and fast DNA purification from 1 µl up to 200 µl of blood without the need of proteolytic digestion steps and heating. Extremely low elution volumes of e.g. 20 µl, starting with 100 µl blood, guarantee highly concentrated, purified DNA (A260/A280 = 1.7 - 1.9) which is suitable for downstream applications such as PCR.
Fig.: Comparison of genomic DNA purified from 100 µl blood run on agarose gel.
Row (1) - (2): chemagic DNA Blood100 Kit;
Row (3) - (4): competitor Kit B, based on magnetic beads;
Row (5) - (6): competitor Kit C, based on magnetic beads; isolated from white blood cells after a centrifugal separation step;
Row (7) - (8): competitor Kit D, based on magnetic beads |
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Manual purification from starting volumes of up to 20 ml of blood can easily be acomplished using the chemagic DNA Blood5k Kit or the chemagic DNA Blood10k Kit. A parallel experiment using ten 10 ml samples of the same human blood was performed with the chemagic DNA Blood10k Kit and a kit based on precipitation, which is used routinely in many labs for genomic DNA isolation from large starting volumes. The next picture shows 0.05 % of eluted DNA obtained in this experiment visualized on an agarose gel.
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Fig.: Comparison of genomic DNA from 10 ml blood.
(M): DNA marker;
(1) - (5): isolated with the
chemagic DNA Blood10k Kit.
(6) - (10): isolated with a precipitation kit. |
The following table shows a comparison of the chemagic DNA Blood10k Kit with other purification methods.
Method |
Yield
[µg] |
Time per Prep.
[min] |
Centrifugation
Steps |
Remarks |
chemagic
DNA Blood10k Kit |
200 - 400 |
45 |
0 |
easy
automatable |
Salting Out |
ca. 250 - 400 |
> 120 |
4 |
difficult
to automate |
Columns |
200 - 400 |
55 - 90 |
5 |
automatable? |
Kits applied on chemagic Automation
The chemagic DNA Blood Kits special (chemagic MSM I) or the Prepito Blood Kits (chemagic Prepito) are designed for automated DNA isolation from fresh or frozen EDTA or Citrat/ACD stabilized whole blood.
Applied on the chemagic MSM I they provide robust low to high throughput DNA isolations from 10 µl up to 10 ml of blood and reveal high molecular DNA with highest yield and purity. The chemagic MSM I/chemagic Blood Kit system is mainly designated for routine laboratories where the combination of speed, reliability and reasonable pricing represents an important parameter for profitability and success.
Prepito DNA Blood Kits on the chemagic Prepito represent a time- and cost-saving alternative without loosing important flexibility. Especially smaller laboratories with lower throughput but even the same high demands to robustness and diversification will be convinced by this new instrument and its related reagents.
DNA isolated with chemagic Kits can be used for a huge variety of downstream applications for e.g. human genetics or HLA-Typing (SSP, SSO, SBT, Southern-Blotting, methylation-sensitive restriction digests, MLPA, Pyrosequencing, Wavescan, SNPlex, TaqMan, MassARRAY etc.).
- Pure DNA from 1 µl - 20 µl Blood Samples
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chemagic DNA Blood10 Kit (article No. 100)
Designed for: manual use
Sample size possible: 1 - 20 µl
Number of preps.: 100 (10 µl blood)
Completion time: ~ 15 min
Typical yields: 0.2 - 0.4 µg DNA (10 µl blood) |
- DNA Isolation from 100 µl - 200 µl Blood Samples
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chemagic DNA Blood100 Kit (article No. 101)
Designed for: manual use
Sample size possible: 100 - 200 µl
Number of preps.: 100 (starting with 100 µl blood)
Completion time: ~ 25 min
Typical yields: 2 - 4 µg DNA (100 µl blood) |
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chemagic DNA Blood Kit special (article No. 721)
Designed for: chemagic MSM I, 96 well format
Sample size possible: 100 µl
Number of preps.: 960
Completion time: ~ 25 min
Typical yields: 2 - 4 µg DNA |
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chemagic DNA Blood Kit special (article No. 717)
Designed for: chemagic MSM I, 96 well format
Sample size possible: 200 µl
Number of preps.: 960
Completion time: ~ 30 min
Typical yields: 4 - 8 µg DNA |
- DNA Isolation from 250 µl Blood Samples
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chemagic DNA Blood250 Kit (article No. 104)
Designed for: manual use
Sample size possible: 250 µl
Number of preps.: 50
Completion time: ~ 30 minutes
Typical yields: 5 - 10 µg DNA |
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Prepito DNA Blood250 Kit (article No. 2002)
Designed for: chemagic Prepito
Sample size possible: 100 - 250 µl
Number of preps.:
Completion time: ~ 55 min
Typical yields: 5 - 10 µg DNA (250 µl blood) |
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chemagic DNA Blood Kit special (article No. 746)
Designed for: chemagic MSM I, 96 well format
Sample size possible: 250 µl
Number of preps.: 960
Completion time: ~ 45 min
Typical yields: 5 - 10 µg DNA |
- DNA Isolation from 350 µl Blood Samples
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chemagic DNA Blood Kit special (article No. 722)
Designed for: chemagic MSM I, 96 well format
Sample size possible: 350 µl
Number of preps.: 960
Completion time: ~ 45 min
Typical yields: 7 - 15 µg DNA |
- Automated DNA Isolation from 350 µl - 600 µl Blood Samples
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chemagic DNA Blood Kit special (article No. 1023)
Designed for: chemagic MSM I, 96 well format
sample size possible: 500 µl
Number of preps.: 480
Completion time: ~ 40 min
Typical yields: 10 - 20 µg DNA |
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Prepito DNA Blood600 Kit (article No. 2004)
Designed for: chemagic Prepito
Sample size possible: 350 - 600 µl
Number of preps.:
Completion time: ~ 45 min
Typical yields: 12 - 24 µg DNA (600 µl blood) |
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Large Volume (600 µl - 10 ml) DNA Isolation from Blood |
- DNA Isolation from 1 ml Blood Samples
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chemagic DNA Blood Kit special (article No. 732)
Designed for: chemagic MSM I, 12 well format
Sample size possible: 1 ml
Number of preps.: 250
Completion time: ~ 30 min
Typical yields: 20 - 40 µg DNA |
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chemagic DNA Blood Kit special (article No. 1050)
Designed for: chemagic MSM I, 24 well format
Sample size possible: 1 - 1,8 ml
Number of preps.: 240
Completion time: ~ 45 min
Typical yields: 20 - 70 µg DNA |
- DNA Isolation from 2 ml Blood Samples
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chemagic DNA Blood Kit special (article No. 711)
Designed for: chemagic MSM I, 12 well format
Sample size possible: 2 ml
Number of preps.: 250
Completion time: ~ 35 min
Typical yields: 40 - 80 µg DNA |
- DNA Isolation from 3 ml Blood Samples
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chemagic DNA Blood Kit special (article No. 763)
Designed for: chemagic MSM I, 12 well format
Sample size possible: 3 ml
Number of preps.: 250
Completion time: ~ 40 min
Typical yields: 50 - 100 µg DNA |
- DNA Isolation from 5 ml Blood Samples
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chemagic DNA Blood5k Kit (article No. 103)
Designed for: manual use
Sample size possible: 3 - 5 ml
Number of preps.: 50
Completion time: ~ 40 min
Typical yields: 70 - 140 µg DNA |
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chemagic DNA Blood Kit special (article No. 703)
Designed for: chemagic MSM I, 12 well format
Sample size possible: 5 ml
Number of preps.: 250
Completion time: ~ 45 min
Typical yields: 70 - 140 µg DNA |
- DNA Isolation from 7 ml Blood Samples
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chemagic DNA Blood Kit special (article No. 715)
Designed for: chemagic MSM I, 12 well format
Sample size possible: 7 ml
Number of preps.: 250
Completion time: ~ 50 min
Typical yields: 100 - 200 µg DNA |
- DNA Isolation from 10 ml Blood Samples
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chemagic DNA Blood10k Kit (article No. 102)
Designed for: manual use
Sample size possible: 7 - 10 ml
Number of preps.: 50
Completion time: ~ 45 min
Typical yields: 150 - 300 µg DNA |
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chemagic DNA Blood Kit special (article No. 704)
Designed for: chemagic MSM I, 12 well format
Sample size possible: 10 ml
Number of preps.: 250
Completion time: ~ 50 min
Typical yields: 150 - 300 µg DNA |
For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.
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