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chemagic Kits for Simultaneous DNA/RNA Isolation
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>> General Information
Comparison chemagic Kits vs. Column Technique
The chemagic Viral DNA/RNA Kit is designed for simultaneous purification of viral DNA and RNA from 200 µl serum or plasma samples. To increase the sensitivity of detection the sample size is scalable. The following gel image shows a comparison of M-PVA Magnetic Bead based technology to conventional column methods.
Fig.: HBV Detection. PCR products amplified from DNA isolated either with the chemagic Viral DNA/RNA Kit (upper row) or a column based kit (lower row).
(1) - (22) PCR products generated from isolated DNA using sera from different patients.
(M): DNA marker. Bands of the upper row and the lower row with the same number are amplificates using eluates obtained from the serum of the same patient.
(N) Negative control. |
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chemagic Viral DNA/RNA Kits on chemagic Automation
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Applied on chemagic Automation, especially regarding large volumes, the kits provide outstanding advantages. The protocol allows direct handling of plasma or serum samples without any intermediary centrifugation or alternative concentration steps. DNA- and RNA-viruses are prepared simultaneously in one extraction. |
Sensitivity Data
DNA/RNA was isolated simultaneously from 9.6 ml minipool plasma samples (96 donors, pool of 100 µl aliquots). The validations were performed at renowned German Red Cross institutes. Different inhouse and commercial TaqMan or Lightcycler assays were used for the detection of viral NAs. A probit analysis of the results revealed the minimal virus concentrations detectable with 95 % probability (see table).
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HAV1 |
HBV2 |
HCV2 |
HIV2 |
Parvo B193 |
Detection limits
[I.U./ml plasma pool] |
2.6 |
0.7 |
5.0 |
33 |
4.6 |
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chemagic Viral DNA/RNA Kit (article No. 193)
Designed for: manual use
Sample size possible: 200 µl
Number of preps.: 100
Completion time: ~ 45 min
Typical sensitivity*: 40 copies/ml |
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Prepito Viral DNA/RNA200 Kit (article No. 2015)
Designed for: chemagic Prepito
Sample size possible: 200 µl
Number of preps.:
Completion time: ~ 40 min |
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chemagic Viral DNA/RNA Kit special (article No. 710)
Designed for: chemagic MSM I, 96 well format
Sample size possible: 200 µl
Number of preps.: 960
Completion time: ~ 40 min
Typical sensitivity*: 40 copies/ml
*achieved for following PCR test on HBV
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chemagic Viral DNA/RNA Kit special (article No. 1005)
Designed for: chemagic MSM I, 12 well format
Sample size possible: 0.8 - 1.2 ml
Number of preps.: 250
Completion time: ~ 45 min
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chemagic Viral DNA/RNA Kit special (article No. 1047)
Designed for: chemagic MSM I, 24 well format
Sample size possible: 2 ml
Number of preps.: 240
Completion time: ~ 60 min
Typical sensitivity: e.g. 1.5 I.U./ml plasma (HCV), 12.3 I.U./ml plasma (HIV)
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chemagic Viral DNA/RNA Kit special (article No. 1001)
Designed for: chemagic MSM I, 12 well format
Sample size possible: 2.4 ml
Number of preps.: 250
Completion time: ~ 45 min
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chemagic Viral DNA/RNA Kit special (article No. 1002)
Designed for: chemagic MSM I, 12 well format
Sample size possible: 4.8 ml
Number of preps.: 250
Completion time: ~ 50 min
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chemagic Viral DNA/RNA Kit special (article No. 749)
Designed for: chemagic MSM I, 12 well format
Sample size possible: 9.6 ml
Number of preps.: 250
Completion time: ~ 55 min
Typical sensitivity: e.g. 6.7 I.U./ml plasma pool (HBV)
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For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.
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