chemagen started its business with the development, functionalisation and production of magnetic particles based on polyvinyl alcohol. We call them M-PVA Magnetic Beads. They represent a new class of particles and allow applications in nearly every scientific or industrial field.
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The surfaces of the M-PVA beads can be functionalized with many different groups and modified with individually tunable loadings. The figure on the left side shows a selection of functionalized, activated and "Ready-to-Use" M-PVA Magnetic Beads offered by chemagen as standard.
The potential of these functionalizations enables extensive and simple modifications with regard to any separation problem. The following listings and the related pages should give an overview about the possibilities.
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Molecular biology and biochemistry
- unspecific isolation of nucleic acids, e.g. for use in PCR
- automated isolation and purification of DNA (analysis diagonstics of genes)
- detection or isolation of specific nucleic acid sequences, e.g. mRNA
- affinity purification of proteins
(e.g. antibodies, lectins, DNA/RNA binding proteins)
- preparation of molecule libraries (e.g. oligonucleotides, DNA, RNA or proteins, but also libraries with smaller molecules) by use of combinatorial strategies
- screening and selection of libraries
Medical diagnostics
- cell separation and cell standardization
- enzyme immunoassay (determination of pathogenic substances)
- antigen or antibody detection by indirect agglutination
- carriers for drug targeting during specialized use of drugs, e.g. in cancer therapy
- removal of pathogenic substances from blood
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»Ready-to-Use« M-PVA Magnetic Beads
M-PVA SAVx; Streptavidin-functionalized
Standard particle sizes: |
M-PVA SAV1: 0.5 - 1 µm (article No. 227)
M-PVA SAV2: 1 - 3 µm (article No. 228) |
Min. quantity delivered: |
2 ml bead suspension |
Concentration: |
25mg/mlMagnetite: 50 - 60 % |
Storage: |
in PBS-Puffer pH 7.2; 0.1 % bovine serum albumine (for Stabilization) 0.05 % Sodium azide |
Binding Capacity: |
M-PVA SAV1
30 - 50 pmol biotinylated protein/mg
at least 400 pmol biotynylated oligonucleotide/mg |
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M- PVA SAV2
30 - 40 pmol biotynlated protein/mg
at least 300 pmol biotinylated oligonucleotide/mg
520 free biotin/mg |
Properties: Streptavidin is immobilized covalently on the surface of superparamagnetic polyvinyl alcohol beads. Their high magnetite content permits the rapid separation of biotinylated target molecules. Because of thehydrophilic nature of the polyvinylalcohol matrix, unspecific binding properties are reduced to a minimum. These beads have a polydisperse size distribution.
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M-PVA OdTx; Oligo(dT)30-functionalized
Standard particle sizes: |
M-PVA OdT1: 0.5 - 1 µm (article No. 230)
M-PVA OdT2: 1 - 3 µm (article No. 231) |
Min. quantity delivered: |
2 ml bead suspension |
Concentration: |
25 mg/ml |
Magnetite: |
50 - 60 % |
Storage: |
in TE-Puffer (10 mM Tris, 1 mM EDTA, pH 8.0); 0.05 % sodium azide |
Binding capacity: |
M-PVA OdT1
1 - 2 µg polyadenylated NA/mg |
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M-PVA OdT2
1 - 2 µg polyadenylated NA/mg |
Properties: Oligo(dT) is immobilized on the surface of superparamagnetic polyvinyl alcohol beads. Their high magnetite content permits the rapid separation of polyadenylated target molecules. For optimal separation results, we recommend the use of a binding buffer with a 0.5 mol/l sodium chloride salt concentration. These beads have a polydisperse size distribution.
Activated M-PVA Magnetic Beads
M-PVA Ak1x NHS-activated beads
Standard particle sizes: |
M-PVA Ak11: 0.5 - 1 µm (article No. 221)
M-PVA Ak12: 1 - 3 µm (article No. 222) |
Min. quantity delivered: |
2 ml bead suspension |
Concentration: Magnetite: |
50 - 60 % |
Activation degree: |
M-PVA Ak11 390 µmol NHS/g
M-PVA Ak12 350 µmol NHS/g |
Binding capacity: |
M-PVA Ak11 8 - 20 mg protein/g
M-PVA Ak12 5 - 15 mg protein/g |
Storage: |
in isopropanol |
Stability: |
at least 1 month at 4 °C |
Properties: These superparamagnetic beads consist of a matrix of carboxylated polyvinyl alcohol, which is subsequently activated with N-hydroxysuccinimidyl (NHS) ester functionalities and can therefore be used for the direct coupling of proteins, nucleic acids or other ligands with amino functionalities.
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M-PVA Ak2x NCO-activated beads
Standard particle sizes: |
M-PVA Ak21: 0.5 - 1 µm (article No. 224)
M-PVA Ak22: 1 - 3 µm (article No. 225) |
Min. quantity delivered: |
2 ml bead suspension |
Concentration: |
25 mg/ml |
Magnetite: |
50 - 60 % |
Activation degree: |
M-PVA Ak21 250 µmol NCO/g
M-PVA Ak22 220 µmol NCO/g |
Storage: |
in dimethylformamide (DMF) |
Stability: |
at least 1 month at 4 °C |
Properties: These superparamagnetic beads consist of a matrix of polyvinyl alcohol, which is subsequently activated by the introduction of isocyanate funcionalities via an eightatom spacer. M-PVA Ak2x can therefore be used for the direct coupling of molecules containing amino or hydroxy functionalities.
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Functionalized M-PVA Magnetic Beads
M-PVA C1x; medium carboxylated beads
Standard particle sizes: |
M-PVA C11: 0.5 - 1 µm (article No. 203)
M-PVA C12: 1 - 3 µm (article No. 204) |
Min. quantity delivered: |
10 ml bead suspension |
Concentration: |
50 mg/ml |
Magnetite: |
50 - 60 % |
Carboxylation degree: |
M-PVA C11 750 µmol COOH/g
M-PVA C12 500 µmol COOH/g |
Storage: |
in H2O |
Properties: These superparamagnetic beads consist of a matrix of polyvinyl alcohol, which is subsequently carboxylated for the easy binding of proteins, nucleic acids or other amino ligands with known coupling methods. Their high magnetite content permits a rapid separation process. The beads have a polydisperse size distribution.
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M-PVA C2x; highly carboxylated beads
Standard particle sizes: |
M-PVA C21: 0.5 - 1 µm (article No. 206)
M-PVA C22: 1 - 3 µm (article No. 207) |
Min. quantity delivered: |
10 ml bead suspension |
Concentration: |
50 mg/ml |
Magnetite: |
50 - 60 % |
Carboxylation degree: |
M-PVA C21 1100 µmol COOH/g
M-PVA C22 950 µmol COOH/g |
Storage: |
in H2O |
Properties: These superparamagnetic beads consist of a matrix of polyvinyl alcohol, which is subsequently carboxylated for the easy binding of proteins, nucleic acids or other amino ligands with known coupling methods. Their high magnetite contentpermits a rapid separation process. The beads have a polydisperse size distribution.
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M-PVA N1x; primary amine functionality
Standard particle sizes: |
M-PVA N11: 0.5 - 1 µm (article No. 209)
M-PVA N12: 1 - 3 µm (article No. 210) |
Min. quantity delivered: |
10 ml bead suspension |
Concentration: |
50 mg/ml |
Magnetite: |
50 - 60 % |
Amination degree: |
M-PVA N11 380 µmol NH3/g
M-PVA N12 310 µmol NH3/g |
Storage: |
in 1 mM EDTA, pH 7.0 |
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Properties: These superparamagnetic beads consist of a matrix of polyvinyl alcohol, which is subsequently aminated using an eight-atom spacer. The beads are suitable for the easy binding of proteins or nucleic acids with known coupling methods (e.g. glutaraldehyde-activation). Their high magnetite content permits a rapid separation process. The beads have a polydisperse size distribution.
M-PVA A0x; aldehyde functionalized
Standard particle sizes: |
M-PVA A01: 0.5 - 1 µm (article No. 218)
M-PVA A02: 1 - 3 µm (article No. 219) |
Min. quantity delivered: |
10 ml bead suspension |
Concentration: |
50 mg/ml |
Magnetite: |
50 - 60 % |
Activation Degree: |
M-PVA A01 250 µmol CHO/g
M-PVA A02 220 µmol CHO/g |
Storage: |
in PBS pH 7.2 (containing 0.02 % sodium azide) |
Binding capacity: |
M-PVA A01 5 - 10 mg protein/g
M-PVA A02 5 - 8 mg protein/g |
Properties: These superparamagnetic beads consist of a matrix of polyvinyl alcohol, which is subsequently activated by the introduction of aldehyde functionalities. M-PVA A0x can therefore be used for the direct binding of proteins or other ligands containing amino functionalities. Their high magnitite content permits a rapid separation process. The beads have a polydisperse size distribution.
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Unmodified M-PVA Magnetic Beads
M-PVA 01x; OH-functionalized Beads
Standard Kit sizes: |
M-PVA 011: 0.5 - 1 µm (article No. 200)
M-PVA 012: 1 - 3 µm (article No. 201) |
Min. quantity delivered: |
10 ml bead suspension |
Concentration: |
50 mg/ml |
Magnetite: |
50/60 % |
Storage: |
in H2O |
Properties: These superparamagnetic beads consist of a matrix of polyvinyl alcohol, which is suitable for a variety of applications e.g. for the binding of proteins to the available hydroxy functionalities with known coupling methods (e.g. activation with tosyl chloride or epichlorhydrine). Their high magnetite content permits a rapid separation process. The beads have a polydisperse size distribution.
For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.
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