ActiPix™ SDI300 Surface Dissolution Imager I ActiPix D100 UV Imaging Detektor I Teknologi I Produktspecifikationer I Tilbehør

ActiPix™ TDA200 HT Nano-Sizing System
Introduction		Catalogues and application notes

The ActiPix™ TDA200  HT Nano-Sizing System is a high precision nano-sizing system consisting of a precision nano-injector, autosampler and detector. Samples are typically stored in the autosampler prior to injection of a few nanolitres of each each sample into a fused capillary of outer diameter typically 200 to 360 µm. Rapid sizing of quantum dots and nanoparticles performed on the ActiPix HT NanoSizing System ActiPix™ product brochure ActiPix TDA200 leaflet

DESCRIPTION OF OPERATION

A plug of the sample, typically 20-100 nL, is injected at the capillary inlet of a specially designed sizing cartridge and driven by application of external pressure along the capillary.

UV absorption of the sample is recorded in the first and the second detection window using the ActiPix™ D100 detector. Whilst the area of the peak is the same, the widths of both peaks are different: the signal from the second window has a greater width and lower amplitude due to Taylor dispersion.

The peaks are fitted with an appropriate peak fitting function using software supplied with the system. The area under the peak corresponds to the amount of the sample injected. The standard deviations are used to calculate the hydrodynamic radius of the sample.

For a more detailed explanation of the theory behind the ActiPix™ Nano-Sizing systems:

1. A novel approach to measurement of hydrodynamic radius for a standard protein using UV area imaging detection
2. Technical Note # TN001: Hydrodynamic radius using Taylor dispersion
3. Application Note # AN001: Measurement of hydrodynamic radius for a standard protein over a wide concentration range
4. Novel instrumentation to gain insight into the aggregation state of proteins

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The ActiPix™ D100 UV Area Imaging System

ActiPix™ SDI300 Surface Dissolution Imager

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ORDERING INFORMATION

Part number: ACTINSIZE

Description

Benchtop Nano-Sizing system with low sample requirement contains:

• ACTID100 system
• Low volume syringe pump
• ActiPix™ sizing cartridge
• 254 nm filter
• Low volume injector valve
• 10 m of fused silica capillary, 200 µm OD, 75 µm ID
• Coupling stand
• 250 µL syringe for buffer delivery
• Syringe for low volume injection
• Fittings kit
• USB data cable
• Tool kit
• Powerful applications software to enable sizing determination of single species, e.g. proteins in solutions    

(System excludes power  cables for local use)

 

MD Scientific ApS • Denmark • tlf. 7027 8565 • fax 7027 8566 • info@md-scientific.dk