|
|
Affinity Chromatography Columns
| AFpak |
· Rigid polymer-based packing material enables high-speed analysis
· Chemically stable bonding groups (spacers) between ligandes and substrates
· Minimizes detachment of ligands, ensuring highly reproducible analysis |
(Standard columns)
| Product name |
Ligand |
Applications |
AFpak AAB-894
AFpak AAF-894
AFpak AAM-894
AFpak AAP-894
AFpak AAV-894
AFpak ABA-894
AFpak ABT-894
AFpak ACA-894
AFpak ACB-894
AFpak ADS-894
AFpak AED-894
AFpak AGA-894
AFpak AGE-894
AFpak AGT-894
AFpak AHR-894
AFpak AIA-894
AFpak ALC-894
AFpak ALS-894
AFpak ANA-894
AFpak AOV-894
AFpak APA-894
AFpak APB-894
AFpak APD-894
AFpak APE-894
AFpak APG-894
AFpak APH-894
AFpak APR-894
AFpak ARC-894
AFpak AST-894
AFpak AWG-894 |
Aminobenzamidine
Acriflavine
5'AMP
Aprotinin
Avidin
Bovine serum albumin (BSA)
Biotin
Concanavalin A (ConA)
Cibacron Blue
Dextran sulfate
Ethylenediamine diacetic acid
N-acetyl-glucosamine
Gelatin
Glutathione
Heparin
Iminodiacetic acid
Lentil lectin
Lysine (LCA)
NAD
Ovomucoid
Protein A
Aminophenyl boronic acid
Procion red
Phosphorylethanolamine
Protein G
Phenyl alanine
Proteamine
RCA-I
Soybean trypsin inhibitor
Wheat germ agglutinin (WGA) |
Serine protease
RNA, DNA, Vitamines
NAD, ATP dependent enzymes
Serine proteases
Biotin derivatives
Chiral Separation(amino acid derivatives)
Avidin
Glycoprotein, polysaccharides
Albumin, NDA dependent enzymes
Lipoproteins, blood coagulation factors
Nucleic acids, serum proteins
Lectins, carbohydrate metabolizing enzymes
Fibronectin
Enzymes related to glutathione
Lipoproteins, blood coagulation factors
Interferon, serum proteins
Glycoproteins, polysaccharides
Plasminogen, plasminogen activator, RNA, DNA
NAD-Dependent dehydrogen
Trypsin-like protease
Human IgG, immune complexes
Nucleic acids and cathecholamines
NAD, NADP, interferon enzymes
C-reactive protein, enzymes
IgG immune complex
Subtilisin Carlsberg
IgM
Glycoproteins, polysaccharides
Trypsin-like proteases
Glycoproteins, polysaccharides |
AFpak ACH-494 AFpak AGO-494 |
Choline oxydase, acetylcholine esterase
Glucose oxydase |
Choline, acetylcholine
Glucose |
| Product name |
Ligand load /gel(g) |
Capacity |
Particle size
(micron) |
Size(mm)
ID x Length |
AAB-894
AAF-894
AAM-894
AAP-894
AAV-894
ABA-894
ABT-894
ACA-894
ACB-894
ADS-894
AED-894
AGA-894
AGE-894
AGT-894
AHR-894
AIA-894
ALC-894
ALS-894
ANA-894
AOV-894
APA-894
APB-894
APD-894
APE-894
APG-894
APH-894
APR-894
ARC-894
AST-894
AWG-894 |
100micro-mol
10micro-mol
10micro-mol
5mg
5mg
20mg
-
15mg
40micro-mol
30mg
30micro-mol
-
30mg
50micro-mol
5mg
70micro-mol
6-7mg
-
10micro-mol
10mg
4mg
800micro-mol
40-60micro-mol
-
4-5mg
50micro-mol
4-5mg
20mg
20mg
14mg |
-
ATP Na 1.8mg/golumn
Lactic dehydronase 1.5mg/g
Trypsin 3mg/g
Biotin 8 micro-g/column
-
Avidin 9mg/g
-
BSA 20mg/column
LDL 5mg/g
-
Lysozyme 0.6mg
Fibronectin 150mg/g
-
Lysozyme 4mg/column
BSA 70mg/column
-
Plasminogen 54micro-g/g
Lactic dehydronase 1.4mg/g
Trypin 100mg/g
IgG Human 20mg/g
Sorbitol 0.2mg/column
BSA 72mg/g
C-reactive protein 0.91mg/g
IgG Humnan 10mg/g
Subtilisin Carlsberg 9mg/g
IgM Human 1.9mg/column
-
Trypin 100mg/g
- |
18
18
18
18
18
6
18
18
18
18
18
18
18
18
18
18
18
18
18
18
18
10
18
18
18
18
18
18
18
18 |
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50
8.0 x 50 |
ACH-494 AGO-494 |
-
- |
-
- |
18
18 |
4.6 x 10
4.6 x 10 |
(Micro column and Semi-micro columns)(Remark)
| Product name |
Size(mm)
ID x Length |
AAB-2B
AAF-2B
AAM-2B
AAP-2B
AAV-2B
ABA-2B
ABT-2B
ACA-2B
ACB-2B
ADS-2B
AED-2B
AGA-2B
AGE-2B
AGT-2B
AHR-2B
AIA-2B
ALC-2B
ALS-2B
ANA-2B
AOV-2B
APA-2B
APB-2B
APD-2B
APE-2B
APG-2B
APH-2B
APR-2B
ARC-2B
AST-2B
AWG-2B |
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50
2.0 x 50 |
| Product name |
Size(mm)
ID x Length |
AAB-2T
AAF-2T
AAM-2T
AAP-2T
AAV-2T
ABA-2T
ABT-2T
ACA-2T
ACB-2T
ADS-2T
AED-2T
AGA-2T
AGE-2T
AGT-2T
AHR-2T
AIA-2T
ALC-2T
ALS-2T
ANA-2T
AOV-2T
APA-2T
APB-2T
APD-2T
APE-2T
APG-2T
APH-2T
APR-2T
ARC-2T
AST-2T
AWG-2T |
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35
2.0 x 35 |
| Product name |
Size(mm)
ID x Length |
AAB-1B
AAF-1B
AAM-1B
AAP-1B
AAV-1B
ABA-1B
ABT-1B
ACA-1B
ACB-1B
ADS-1B
AED-1B
AGA-1B
AGE-1B
AGT-1B
AHR-1B
AIA-1B
ALC-1B
ALS-1B
ANA-1B
AOV-1B
APA-1B
APB-1B
APD-1B
APE-1B
APG-1B
APH-1B
APR-1B
ARC-1B
AST-1B
AWG-1B |
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50
1.0 x 50 |
| Product name |
Size(mm)
ID x Length |
AAB-1T
AAF-1T
AAM-1T
AAP-1T
AAV-1T
ABA-1T
ABT-1T
ACA-1T
ACB-1T
ADS-1T
AED-1T
AGA-1T
AGE-1T
AGT-1T
AHR-1T
AIA-1T
ALC-1T
ALS-1T
ANA-1T
AOV-1T
APA-1T
APB-1T
APD-1T
APE-1T
APG-1T
APH-1T
APR-1T
ARC-1T
AST-1T
AWG-1T |
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35
1.0 x 35 |
| Product name |
Size(mm)
ID x Length |
AAB-M8B
AAF-M8B
AAM-M8B
AAP-M8B
AAV-M8B
ABA-M8B
ABT-M8B
ACA-M8B
ACB-M8B
ADS-M8B
AED-M8B
AGA-M8B
AGE-M8B
AGT-M8B
AHR-M8B
AIA-M8B
ALC-M8B
ALS-M8B
ANA-M8B
AOV-M8B
APA-M8B
APB-M8B
APD-M8B
APE-M8B
APG-M8B
APH-M8B
APR-M8B
ARC-M8B
AST-M8B
AWG-M8B |
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50
0.8 x 50 |
| Product name |
Size(mm)
ID x Length |
AAB-M8T
AAF-M8T
AAM-M8T
AAP-M8T
AAV-M8T
ABA-M8T
ABT-M8T
ACA-M8T
ACB-M8T
ADS-M8T
AED-M8T
AGA-M8T
AGE-M8T
AGT-M8T
AHR-M8T
AIA-M8T
ALC-M8T
ALS-M8T
ANA-M8T
AOV-M8T
APA-M8T
APB-M8T
APD-M8T
APE-M8T
APG-M8T
APH-M8T
APR-M8T
ARC-M8T
AST-M8T
AWG-M8T |
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35
0.8 x 35 |
| Product name |
Size(mm)
ID x Length |
AAB-M5B
AAF-M5B
AAM-M5B
AAP-M5B
AAV-M5B
ABA-M5B
ABT-M5B
ACA-M5B
ACB-M5B
ADS-M5B
AED-M5B
AGA-M5B
AGE-M5B
AGT-M5B
AHR-M5B
AIA-M5B
ALC-M5B
ALS-M5B
ANA-M5B
AOV-M5B
APA-M5B
APB-M5B
APD-M5B
APE-M5B
APG-M5B
APH-M5B
APR-M5B
ARC-M5B
AST-M5B
AWG-M5B |
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50
0.5 x 50 |
| Product name |
Size(mm)
ID x Length |
AAB-M5T
AAF-M5T
AAM-M5T
AAP-M5T
AAV-M5T
ABA-M5T
ABT-M5T
ACA-M5T
ACB-M5T
ADS-M5T
AED-M5T
AGA-M5T
AGE-M5T
AGT-M5T
AHR-M5T
AIA-M5T
ALC-M5T
ALS-M5T
ANA-M5T
AOV-M5T
APA-M5T
APB-M5T
APD-M5T
APE-M5T
APG-M5T
APH-M5T
APR-M5T
ARC-M5T
AST-M5T
AWG-M5T |
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35
0.5 x 35 |
| Product name |
Size(mm)
ID x Length |
AAB-M3B
AAF-M3B
AAM-M3B
AAP-M3B
AAV-M3B
ABA-M3B
ABT-M3B
ACA-M3B
ACB-M3B
ADS-M3B
AED-M3B
AGA-M3B
AGE-M3B
AGT-M3B
AHR-M3B
AIA-M3B
ALC-M3B
ALS-M3B
ANA-M3B
AOV-M3B
APA-M3B
APB-M3B
APD-M3B
APE-M3B
APG-M3B
APH-M3B
APR-M3B
ARC-M3B
AST-M3B
AWG-M3B |
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50
0.3 x 50 |
| Product name |
Size(mm)
ID x Length |
AAB-M3T
AAF-M3T
AAM-M3T
AAP-M3T
AAV-M3T
ABA-M3T
ABT-M3T
ACA-M3T
ACB-M3T
ADS-M3T
AED-M3T
AGA-M3T
AGE-M3T
AGT-M3T
AHR-M3T
AIA-M3T
ALC-M3T
ALS-M3T
ANA-M3T
AOV-M3T
APA-M3T
APB-M3T
APD-M3T
APE-M3T
APG-M3T
APH-M3T
APR-M3T
ARC-M3T
AST-M3T
AWG-M3T |
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35
0.3 x 35 |
Chiral Separation Columns - ORpak CD
| ORpak CD |
· alpha-, beta-, gamma-cyclodextrin derivatives bonded
· Sparates chiral isomers based on conformity to cyclodextrin derivatives steric structure
· General columns chiral separation |
(Standard columns)
| Product name |
Functional group |
Particle size
(micron) |
Size(mm)
ID x Length |
Max. usable
pressure(MPa) |
Max. usable
flow rate(mL/min) |
ORpak CDA-453 HQ
ORpak CDB-453 HQ
ORpak CDC-453 HQ
ORpak CDBS-453
ORpak CD-G |
alpha-Cyclodextrin derivertive
beta-Cyclodextrin derivertive
gamma-Cyclodextrin derivertive
beta-Cyclodextrin derivertive
Guard column |
6
6
6
3
6 |
4.6 x 150
4.6 x 150
4.6 x 150
4.6 x 150
4.6 x 10 |
4
4
4
20
- |
1.0
1.0
1.0
1.2
- |
| Product name |
Usable
temp. range(deg-C) |
Usable
pH range |
Usable
salt conc. |
Shipping solvent |
CDA-453 HQ
CDB-453 HQ
CDC-453 HQ
CDBS-453
CD-G |
4 to 60
4 to 60
4 to 60
4 to 60
4 to 60 |
3 to 10
3 to 10
3 to 10
2 to 7
3 to 10 |
< 3M
< 3M
< 3M
< 1M
< 3M |
H2O
1.7% TEA-scetate buffer 0.1M NaCl(pH4.0)/CH3CN=80/20
1.0% Acetic acid + 0.2M NaCl aq./CH3CN=90/10
1.0% Acetic acid + 0.2M NaCl aq./CH3CN=70/30
H2O |
Chiral Separation Columns - ORpak CRX-853
| CRX-853 |
· L-amino acid derivatives bonded
· Sparates chiral isomers by difference capacity of metal complex formation between sample and eluent
· For the separation of amino acids, hydroxylic acids, and derivativees thereof |
(Standard column)
| Product name |
Functional group |
Particle size
(micron) |
Size(mm)
ID x Length |
ORpak CRX-853
ORpak CRX-G |
L-amino acid derivative
Guard column |
6
6 |
8.0 x 50
4.6 x 10 |
| Product name |
Max. usable
pressure(MPa) |
Max. usable
flow rate(mL/min) |
Usable temp.
range(deg-C) |
Shipping solvent |
CRX-853
CRX-G |
1.5
- |
2.0
- |
4 to 60
4 to 60 |
0.25mM CuSO4 aq.
0.25mM CuSO4 aq. |
Chiral Separation Columns - AFpak ABA-894
| ABA-894 |
· Bovine serum albumin(BSA) bonded
· Sparates chiral isomers based on interactions between the isomers captured by the hydrophoric pockets of BSA and surrounding amino acid residue.
· For the separation of chiral isomers od amino acids and carboxylic acids |
(Standard column)
| Product name |
Functional group |
Particle size
(micron) |
Size(mm)
ID x Length |
Shipping solvent |
Usable
temp. range(deg-C) |
| AFpak ABA-894 |
Bovine serum albumin |
6 |
8.0 x 50 |
50mM Tris-HCl buffer
+ 150mM NaCl
+ 0.02% NaN3(pH7.4) |
5 to 40 |
Principle of Affinity Chromatography
The principle of affinity chromatography is as follows:
1) Inject a sample into an initially equilibrated affinity chromatography column(AFpak).
2) Only the substances with affinity for the ligand are retained in the column.
3) Other substances with no affinity for the ligand are eluted from the column.
4) The substances retained in the column can be eluted from the column by changing pH or salt or organic solvent concentration of the eluent.
Affinity chromatography is widely used as a means of separation and purification with specific properties.

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