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HPLC kolonner fra

 
Showa Denko
Shodex logo
 
MD Scientific er autoriseret forhandler i Danmark for
Showa Denkos (Shodex) produkter

 

Normal-Reversed Phase - Polymer-Based kolonner

 

  shodex columns
     
 

Startside for Shodex kolonner

Kataloger og brochurer


Polymer-Based Columns - RSpak
RP18-415 · For reversed phase analysis of proteins and peptides
- large pore size
DS-613,413 · For reversed phase analysis of highly hydrophilic substances, which cannot be retained well by ODS columns.
RP18-613,413 · High-performance (high-theoretical-plate-number) version of DS-613 and DS-413
DE Series · Widest application because of similar polarity compared with ODS columns
· Wide pH range enabling use in 100% water
DM-614 · For the analysis of amino acids and water-soluble vitamins
NN-814,614,414 · The packing material contains a trace amount of sulfo groups, it supports multi mode (reversed phase and cation exchange) analysis
· Ideal for complex samples cantaining neutral and ionic substances
JJ-50

· The packing material contains a trace amount of quanternary ammonium groups, it supports multi mode (reversed phase and anion exchange) analysis
· Ideal for complex samples cantaining neutral and ionic substances

DC-613 · For the normal phase analysis of saccharides

RSpak RP18-415, 613, 413
(Standard columns)
Product name Plate number
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Shipping solvent
RSpak RP18-415
RSpak RP18-613
RSpak RP18-413
RSpak RP18-G
> 5,000
> 13,000
> 11,000
Guard column
6
3.5
3.5
6
4.6 x 150
6.0 x 150
4.6 x 150
4.6 x 10
CH3CN/H2O=95/5
CH3CN/THF/H2O=30/30/40
CH3CN/THF/H2O=30/30/40
CH3CN/THF/H2O=30/30/40
Product name Max. usable
pressure(MPa)
Max. usable
flow rate(mL/min)
Usable temp.
range(deg-C)
Usable
pH range
Usable salt
conc.
RP18-415
RP18-613
RP18-413
RP18-G
22
22
22
-
1.5
1.5
1.0
-
RT to 50
RT to 50
RT to 50
RT to 50
1 to 13
1 to 13
1 to 13
1 to 13
< 0.5M
< 0.5M
< 0.5M
< 0.5M
When you do not use a column, please keep it between pH 2 to 12.
In the case of keeping a column for a long time, please replace with the shipping solvent in the column.

Eluent conditions:
Product name Toluene
Chloroform etc.
Methanol Acetonitrile THF Water solusiton
RP18-415
RP18-613
RP18-413

0 to 50%
0 to 50%
0 to 50%

0 to 100%
30 to 100%
30 to 100%

0 to 100%
10 to 100%
10 to 100%

0 to 50%
5 to 100%
5 to 100%

0 to 100%
cannot use 100% Water solusiton
cannot use 100% Water solusiton


RSpak DS-613, 413
(Standard columns)
Product name Plate number
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Shipping solvent
RSpak DS-613
RSpak DS-413
RSpak DS-G
> 6,500
> 11,000
Guard column
6
4
10
6.0 x 150
4.6 x 150
4.6 x 10
CH3CN/THF/H2O=30/40/30
CH3CN/THF/H2O=30/40/30
CH3CN/THF/H2O=30/40/30
Product name
Max. usable
pressure(MPa)
Max. usable
flow rate(mL/min)
Usable temp.
range(deg-C)
Usable
pH range
Usable
salt conc.
DS-613
DS-413
DS-G
10
20
-
3.0
1.0
-
RT to 80
20 to 50
RT to 80
2 to 12
*1 to 13
*1 to 13
< 0.5M
< 0.5M
< 0.5M
When you do not use a column, please keep it between pH 2 to 12.
In the case of keeping a column for a long time, please replace with the shipping solvent in the column.


RSpak DE Series
(Standard columns)
Product name Plate number
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Shipping solvent
RSpak DE-613
RSpak DE-413
RSpak DE-413L
RSpak DE-413S
RSpak DE-G
RSpak DE-213
RSpak DE-SG
> 7,000
> 11,000
> 17,000
> 3,000
Guard column
> 8,000
Guard column
6
4
4
4
10
4
6
6.0 x 150
4.6 x 150
4.6 x 250
4.6 x 50
4.6 x 10
2.0 x 150
2.0 x 10
H2O
CH3CN/H2O=50/50
CH3CN/H2O=50/50
CH3CN/H2O=50/50
H2O
CH3CN/H2O=50/50
CH3CN/H2O=50/50
Product name
Max. usable
pressure(MPa)
Max. usable
flow rate(mL/min)
Usable temp.
range(deg-C)
Usable
pH range
Usable
salt conc.
Applications
DE-613
DE-413
DE-413L
DE-413S
DE-G
DE-213
DE-SG
7
15
18
-
-
12
-
3.0
1.2
1.2
-
-
0.4
-
RT to 70
RT to 60
RT to 60
RT to 60
RT to 70
RT to 60
RT to 60
2 to 12
2 to 12
2 to 12
2 to 12
2 to 12
2 to 11
2 to 11
< 0.5M
< 0.5M
< 0.5M
< 0.5M
< 0.5M
< 0.5M
< 0.5M



Column-switching

LC/MS analyses


(Micro column and Semi-micro columns)
Product name Size(mm)
ID x Length
Standard
column
DE413-2E
DE413-2D
DE413-2B
DE413-2T
2.0 x 250
2.0 x 150
2.0 x 50
2.0 x 35
DE-413
DE413-1E
DE413-1D
DE413-1B
DE413-1T
1.0 x 250
1.0 x 150
1.0 x 50
1.0 x 35
DE-413
DE413-M8D
DE413-M8B
DE413-M8T
0.8 x 150
0.8 x 50
0.8 x 35
DE-413
DE413-M5D
DE413-M5B
DE413-M5T
0.5 x 150
0.5 x 50
0.5 x 35
DE-413
DE413-M3D
DE413-M3B
DE413-M3T
0.3 x 150
0.3 x 50
0.3 x 35
DE-413

(Preparative Columns)
Product name Plate number
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Standard
column
RSpak DE-2013
RSpak DE-LG
> 10,000
Guard column
12
12
20.0 x 300
8.0 x 50
DE-613
Guard column
RSpak DE-5013
RSpak DE-LLG
-
Guard column
12
12
50.0 x 300
20.0 x 100
DE-613
Guard column

RSpak DM-614, 2014, 5014
(Standard columns)
Product name Plate number
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Shipping solvent
RSpak DM-614
RSpak DM-G
> 4,500
Guard column
10
12
6.0 x 150
4.6 x 10
5mM H3PO4
5mM H3PO4
Product name Max. usable
pressure(MPa)
Max. usable
flow rate(mL/min)
Usable temp.
range(deg-C)
Usable
pH range
Usable
salt conc.
DM-614
DM-G
2
-
2.0
-
RT to 60
-
2 to 10
-
< 0.5M
-


(Preparative Columns)
Product name Plate number
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Standard
column
RSpak DM-2014
RSpak DM-LG
> 5,000
Guard column
12
12
20.0 x 300
8.0 x 50
DM-614
Guard column
RSpak DM-5014
RSpak DM-LLG
-
Guard column
12
12
50.0 x 300
20.0 x 100
DM-614
Guard column


RSpak NN Series

(Standard columns)
Product name Plate number
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Shipping solvent
RSpak NN-814
RSpak NN-614
RSpak NN-G
RSpak NN-414
> 9,000
> 4,000
Guard column
> 6,000
10
10
10
10
8.0 x 250
6.0 x 150
6.0 x 50
4.6 x 150
0.1M Sodium phosphate buffer (pH3.0)
0.1M Sodium phosphate buffer (pH3.0)
0.1M Sodium phosphate buffer (pH3.0)
0.1M Sodium phosphate buffer (pH3.0)
Product name Max. usable
pressure(MPa)
Max. usable
flow rate(mL/min)
Usable temp.
range(deg-C)
Usable
pH range
Usable
salt conc.
NN-814
NN-614
NN-G
NN-414
2.0
1.5
-
1.2
1.5
1.0
-
0.5
10 to 50
10 to 50
10 to 50
10 to 50
2 to 12
2 to 12
2 to 12
2 to 12
< 0.5M
< 0.5M
< 0.5M
< 0.5M


(Micro column and Semi-micro columns)(Remark)
Product name Size(mm)
ID x Length
Standard
column
NN414-2D
NN414-2B
NN414-2T
2.0 x 150
2.0 x 50
2.0 x 35
NN-414
NN414-1D
NN414-1B
NN414-1T
1.0 x 150
1.0 x 50
1.0 x 35
NN-414
NN414-M8D
NN414-M8B
NN414-M8T
0.8 x 150
0.8 x 50
0.8 x 35
NN-414
NN414-M5D
NN414-M5B
NN414-M5T
0.5 x 150
0.5 x 50
0.5 x 35
NN-414
NN414-M3D
NN414-M3B
NN414-M3T
0.3 x 150
0.3 x 50
0.3 x 35
NN-414

RSpak JJ-50
(Standard columns)
Product name Plate number
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Shipping solvent
RSpak JJ-50 4D
RSpak JJ-50 2D
> 4,500
> 3,500
5
5
4.6 x 150
2.0 x 150
CH3CN/H2O=60/40
CH3CN/H2O=60/40
Product name Max. usable
pressure(MPa)
Max. usable
flow rate(mL/min)
Usable temp.
range(deg-C)
Usable
pH range
Usable
salt conc.
JJ-50 4D
JJ-50 2D
9.0
9.0
1.2
0.3
10 to 60
10 to 60
2 to 10
2 to 10
< 0.1M
< 0.1M

RSpak DC-613, 2013, 5013
(Standard columns)
Product name Plate number
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Shipping solvent
RSpak DC-613
RSpak DC-G
> 5,500
Guard column
6
10
6.0 x 150
4.6 x 10
CH3CN/H2O=70/30
CH3CN/H2O=70/30
Product name Max. usable
pressure(MPa)
Max. usable
flow rate(mL/min)
Usable temp.
range(deg-C)
Usable
pH range
Usable
salt conc.
DC-613
DC-G
5
-
3.0
-
40 to 85
40 to 85
2 to 14
2 to 14
< 0.5M
< 0.5M


(Preparative Columns)
Product name Plate number
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Standard
column
RSpak DC-2013
RSpak DC-LG
> 6,000
Guard column
10
10
20.0 x 300
8.0 x 50
DC-613
Guard column
RSpak DC-5013
RSpak DC-LLG
-
Guard column
10
10
50.0 x 300
20.0 x 100
DC-613
Guard column

Polymer-Based Columns - Asahipak

ODP-50
C8P-50
C4P-50
· The large pore size supports the analysis of preteins, peptides, and amno acids
· Wide pH range (pH2-13)
· Supports use in 100% water
· Especially good for the analysis of basic molecules
ODP-40 · High performance type of the ODP-50
NH2P-50 · For the normal phase analysis of saccharides
· Polymer-based packing material provides excellent chemical stability and resistance to time degradation
· Washing with a basic solvent
· Wide pH range (pH2-13)

ODP-40, ODP-50, C8P-50, C4P-50
(Standard Columns)
Product name
Plate number
(TPN/column)
Particle size
(micron)
Carbon
load(%)
Size(mm)
ID x Length
Shipping solvent
Asahipak ODP-40 4D
Asahipak ODP-40 4E
> 11,000
> 17,000
4
4
17
17
4.6 x 150
4.6 x 250
CH3CN/H2O=65/35
CH3CN/H2O=65/35
Asahipak ODP-50 6D
Asahipak ODP-50 6E
Asahipak ODP-50G 6A
> 9,000
> 14,000
Guard column
5
5
5
17
17
17
6.0 x 150
6.0 x 250
6.0 x 10
CH3CN/H2O=65/35
CH3CN/H2O=65/35
CH3CN/H2O=65/35
Asahipak ODP-50 4B
Asahipak ODP-50 4D
Asahipak ODP-50 4E
Asahipak ODP-50G 4A
> 2,500
> 9,000
> 14,000
Guard column
5
5
5
5
17
17
17
17
4.6 x 50
4.6 x 150
4.6 x 250
4.6 x 10
CH3CN/H2O=65/35
CH3CN/H2O=65/35
CH3CN/H2O=65/35
CH3CN/H2O=65/35
Asahipak ODP-50 2D
Asahipak ODP-50G 2A
> 5,000
Guard column
5
5
17
17
2.0 x 150
2.0 x 10
CH3CN/H2O=65/35
CH3CN/H2O=65/35
Asahipak C8P-50 4D
Asahipak C8P-50 4E
Asahipak C8P-50G 4A
> 7,000
> 11,000
Guard column
5
5
5
10
10
10
4.6 x 150
4.6 x 250
4.6 x 10
CH3CN/H2O=65/35
CH3CN/H2O=65/35
CH3CN/H2O=65/35
Asahipak C4P-50 4D
Asahipak C4P-50 4E
Asahipak C4P-50G 4A
> 6,000
> 9,000
Guard column
5
5
5
6
6
6
4.6 x 150
4.6 x 250
4.6 x 10
CH3CN/H2O=65/35
CH3CN/H2O=65/35
CH3CN/H2O=65/35
Product name
Max. usable
pressure(MPa)
Max. usable
flow rate(mL/min)
Usual
flow rate(mL/min)
Usable temp.
range(deg-C)
Usable
pH range
ODP-40 4D
ODP-40 4E
9
13
0.3 to 0.6
0.3 to 0.6
0.8
0.8
4 to 60
4 to 60
2 to 13
2 to 13
ODP-50 6D
ODP-50 6E
ODP-50G 6A
15
15
-
0.5 to 1.0
0.5 to 1.0
-
2.5
2.5
-
4 to 60
4 to 60
4 to 60
2 to 13
2 to 13
2 to 13
ODP-50 4B
ODP-50 4D
ODP-50 4E
ODP-50G 4A
5
15
15
-
0.4 to 0.6
0.5 to 0.8
0.5 to 0.8
-
1.5
1.5
1.5
-
4 to 60
4 to 60
4 to 60
4 to 60
2 to 13
2 to 13
2 to 13
2 to 13
ODP-50 2D
ODP-50G 2A
12
-
0.2
-
0.4
-
4 to 60
4 to 60
2 to 13
2 to 13
C8P-50 4D
C8P-50 4E
C8P-50G 4A
15
15
-
0.5 to 0.8
0.5 to 0.8
-
1.0
1.0
-
4 to 60
4 to 60
4 to 60
2 to 13
2 to 13
2 to 13
C4P-50 4D
C4P-50 4E
C4P-50G 4A
15
15
-
0.5 to 0.8
0.5 to 0.8
-
1.0
1.0
-
4 to 60
4 to 60
4 to 60
2 to 13
2 to 13
2 to 13

(Micro column and Semi-micro columns)
Product name
Size(mm)
ID x Length
Standard
column
ODP40-2E
ODP40-2D
ODP40-2B
ODP40-2T
2.0 x 250
2.0 x 150
2.0 x 50
2.0 x 35
ODP-40
ODP40-1E
ODP40-1D
ODP40-1B
ODP40-1T
1.0 x 250
1.0 x 150
1.0 x 50
1.0 x 35
ODP-40
ODP40-M8D
ODP40-M8B
ODP40-M8T
0.8 x 150
0.8 x 50
0.8 x 35
ODP-40
ODP40-M5D
ODP40-M5B
ODP450-M5T
0.5 x 150
0.5 x 50
0.5 x 35
ODP-40
ODP40-M3D
ODP40-M3B
ODP40-M3T
0.3 x 150
0.3 x 50
0.3 x 35
ODP-40

(Preparative Columns)(Remark)
Product name
Plate number
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Standard
column
Asahipak ODP-50 10E
Asahipak ODP-90 20F
Asahipak ODP-130 28F
Asahipak ODP-130G 7B
Asahipak ODP-130G 20C
> 10,000
> 9,000
> 9,000
Guard column
Guard column
5
9
13
13
13
10.0 x 250
20.0 x 300
28.0 x 300
7.5 x 50
20.0 x 100
ODP-50
ODP-50
ODP-50
Guard column
Guard column
Asahipak C8P-50 10E
Asahipak C8P-50G 7B
Asahipak C4P-50 10E
Asahipak C4P-50G 7B
> 8,000
Guard column
> 7,000
Guard column
5
5
5
5
10.0 x 250
7.5 x 50
10.0 x 250
7.5 x 50
C8P-50
Guard column
C4P-50
Guard column

NH2P-50
(Standard Columns)
Product name
Plate number
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Shipping solvent
Asahipak NH2P-50 4D
Asahipak NH2P-50 4E
Asahipak NH2P-50G 4A
> 5,500
> 7,500
Guard column
5
5
5
4.6 x 150
4.6 x 250
4.6 x 10
CH3CN/H2O=75/25
CH3CN/H2O=75/25
CH3CN/H2O=75/25
Asahipak NH2P-50 2D
Asahipak NH2P-50G 2A
> 3,500
Guard column
5
5
2.0 x 150
2.0 x 10
CH3CN/H2O=75/25
CH3CN/H2O=75/25
Asahipak NH2P-LF
Line filter
5
8.0 x 75
CH3CN/H2O=75/25
Product name
Max. usable
pressure(MPa)
Usual
flow rate(mL/min)
Max. usable
flow rate (mL/min)
Usable temp.
range(deg-C)
Usable
pH range
NH2P-50 4D
NH2P-50 4E
NH2P-50G 4A
15
9
-
0.5 to 1.0
0.4 to 0.8
-
1.5
1.5
-
4 to 50
4 to 50
4 to 50
2 to 13
2 to 13
2 to 13
NH2P-50 2D
NH2P-50G 2A
12
-
0.2
-
0.4
-
4 to 50
4 to 50
2 to 13
2 to 13
NH2P-LF - - - 4 to 50
2 to 13

(Micro column and Semi-micro columns)
Product name
Size(mm)
ID x Length
Standard
column
NH2P40-2E
NH2P40-2D
NH2P40-2B
NH2P40-2T
2.0 x 250
2.0 x 150
2.0 x 50
2.0 x 35
NH2P-50
NH2P40-1E
NH2P40-1D
NH2P40-1B
NH2P40-1T
1.0 x 250
1.0 x 150
1.0 x 50
1.0 x 35
NH2P-50
NH2P40-M8D
NH2P40-M8B
NH2P40-M8T
0.8 x 150
0.8 x 50
0.8 x 35
NH2P-50
NH2P40-M5D
NH2P40-M5B
NH2P40-M5T
0.5 x 150
0.5 x 50
0.5 x 35
NH2P-50
NH2P40-M3D
NH2P40-M3B
NH2P40-M3T
0.3 x 150
0.3 x 50
0.3 x 35
NH2P-50

(Preparative Columns)
Product name
Plate number
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Standard
column
Asahipak NH2P-50 10E
Asahipak NH2P-90 20F
Asahipak NH2P-130 28F
Asahipak NH2P-130G 7B
> 10,000
> 10.000
> 1,000
Guard column
5
9
13
13
10.0 x 250
20.0 x 300
28.0 x 300
7.5 x 50
NH2P-50
NH2P-50
NH2P-50
Guard column

 

Polymer-Based Columns - ODP2 HP

ODP2 HP · Improved theoretical plate number compared with general polymer-based columns, almost twice as good
· Retain both polar and non-polar small molecules
· No protein retention
- can analyze small molecules in the presence of proteins
· Ideal for LC/MS analysis
- retain sharp peak shape even in low salt concentration

(Standard Column)
Product name
Plate number
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Shipping solvent
ODP2 HP-4B
ODP2 HP-4D
ODP2 HP-4E
ODP2 HPG-4A

> 3,500
> 13,000
> 17,000
Guard column

5
5
5
5
4.6 x 50
4.6 x 150
4.6 x 250
4.6 x 10

CH3CN/H2O=45/55
CH3CN/H2O=45/55
CH3CN/H2O=45/55
CH3CN/H2O=45/55

ODP2 HP-2B
ODP2 HP-2D
ODP2 HPG-2A

> 3,000
> 7,000
Guard column

5
5
5
2.0 x 50
2.0 x 150
2.0 x 10
CH3CN/H2O=45/55
CH3CN/H2O=45/55
CH3CN/H2O=45/55
ODP2 HP-4GC

Cartridge guard

5 4.0 x 10
H2O
HLD-4GC

Holder

- -
-
Product name
Max. usable
pressure(MPa)
Max. usable
flow rate(mL/min)

Usable temp.
range(deg-C)

Usable
pH range
Usable salt
conc.
ODP2 HP-4B
ODP2 HP-4D
ODP2 HP-4E
ODP2 HPG-4A
15
15
15
-
1.5
1.5
1.0
-
20 to 60
20 to 60
20 to 60
20 to 60
3 to 12
3 to 12
3 to 12
3 to 12
> 0.1M
> 0.1M
> 0.1M
> 0.1M
ODP2 HP-2B
ODP2 HP-2D
ODP2 HPG-2A
12
15
-
0.3
0.3
-
20 to 60
20 to 60
20 to 60
3 to 12
3 to 12
3 to 12
> 0.1M
> 0.1M
> 0.1M
ODP2 HP-4GC - 1.5 20 to 60 3 to 12
> 0.1M
HLD-4GC - - - - -

 

Polymer-based Column for HighTemperature Analysis - ET-RP1

ET-RP1 · High-temperature analysis up to 150 deg-C
· High resolution and fast analysis by high temperature

(Standard Column)
Product name
Plate number*
(TPN/column)
Particle size
(micron)
Size(mm)
ID x Length
Shipping solvent
ET-RP1 4D

> 11,000

4 4.6 x 150

CH3CN/H2O=65/35

ET-RP1 3D

> 9,000

4 3.0 x 150

CH3CN/H2O=65/35

Product name
Max. usable
pressure(MPa)

Usable temp.
range(deg-C)

Usable
pH range
ET-RP1 4D 15 20 to 150 4 to 9
ET-RP1 3D 15 20 to 150 4 to 9
*30deg-C

 

 


Characteristics and Application of Normal and Reversed Phase Columns

RSpak RP18-415 has a pore size of approximately 450 angstrom and are suitable for the analysis of proteins. The Asahipak ODP, C8P and C4P have a pore size of approximately 250 angstrom and are suitable for peptides.
The RSpak DE-613 and DE-413 have a pore size of approximately 100 angstrom can be used for the analysis of a low molecular weight substances such as oligopeptides. Should none the DE-613, 413 or ODS columns give satisfactory results due the non-retention of the sample then the use of the RSpak RP18-413 or the RP18-613 which have a lower polarity can be recommended.

reversed-phase

 

For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.

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MD Scientific
MD Scientific
MD Scientific
MD Scientific ApS • Denmark • tlf. 7027 8565 • fax 7027 8566 • info@md-scientific.dk