Shodex kolonner

• Packing Materials for Shodex Columns
• Guidelines for Shodex Column Selection
• General Care to Use Packed Columns
• Column Cleaning Procedure
• Column Test Conditions

Separation type	Products		Base material	Functional group
Reversed & Normal phase (Polymer-based)	Asahipak	ODP-50 ODP-40	Polyvinyl alcohol	Octadecyl
	Asahipak	C8P-50	Polyvinyl alcohol	Octyl
	Asahipak	C4P-50	Polyvinyl alcohol	Butyl
		ODP2 HP	Polyhydroxymethacrylate	-
	RSpak	RP18	Styrene divinylbenzene copolymer	-
	RSpak	DS	Styrene divinylbenzene copolymer	-
	RSpak	DE	Polymethacrylate	-
	RSpak	GOLF-413 CARB	Polymethacrylate	-
	RSpak	DM-413	Polyhydroxymethacrylate	-
	RSpak	NN	Polyhydroxymethacrylate	Sulfo
	RSpak	JJ-50	Polyvinyl alcohol	Quaternary ammonium
	Asahipak	NH2P-50	Polyvinyl alcohol	Polyamino
	RSpak	DC-613	Styrene divinylbenzene copolymer	Sulfo(Na+)
Reversed & Normal phase (Silica-based)	ODSpak	F	Silica	Octadecyl
	Silica	C18M C18P	Silica	Octadecyl
	Silicapak	E-411	Silica	-
	Silica	5SIL	Silica	-
	Silica	5C8	Silica	Octyl
	Silica	5C4	Silica	Butyl
	Silica	5TMS	Silica	Trimethylsilyl
	Silica	5CN	Silica	Cyanopropyl
	Silica	5NH	Silica	Aminopropyl
	Silica		Silica	Nitrophenylethyl
	Silica	5NPYE	Silica	Pyrenylethyl
Cation-exchange	IEC	SP-825	Polyhydroxymethacrylate	Sulfopropyl
	IEC	SP-420N	Polyhydroxymethacrylate	Sulfopropyl
	IEC	CM-825	Polyhydroxymethacrylate	Carboxymethyl
	Asahipak	ES-502C	Polyvinyl alcohol	Carboxymethyl
Anion-exchange	IEC	QA-825	Polyhydroxymethacrylate	Quaternary ammonium
	IEC	DEAE-825	Polyhydroxymethacrylate	Diethylaminoethyl
	IEC	DEAE3N	Polyhydroxymethacrylate	Diethylaminoethyl
	Asahipak	ES-502N	Polyvinyl alcohol	Diethylaminoethyl
	AXpak	WA-624	Polyhydroxymethacrylate	Diethylaminoethyl
Hydrophobic	HIC	PH-814	Polyhydroxymethacrylate	Phenyl
Ion chromatography (Cation Analysis)	IC	YS-50	Polyvinyl alcohol	Quaternary ammonium
	IC	YK-421	Silica	Carboxyl
	IC	Y-521	Styrene divinylbenzene copolymer	Sulfo
	IC	T-521	Styrene divinylbenzene copolymer	Sulfo
	IC	R-621	Styrene divinylbenzene copolymer	Sulfo
Ion chromatography (Anion Analysis)	IC	NI-424	Polyhydroxymethacrylate	Quaternary ammonium
	IC	I-524A	Polyhydroxymethacrylate	Quaternary ammonium
	IC	SI	Polyvinyl alcohol	Quaternary ammonium
	WINE	VH-anion	Polyvinyl alcohol	Quaternary ammonium
Chiral separation	ORpak	CDA-453 HQ	Polyhydroxymethacrylate	alpha-Cyclodextrin derivertive
	ORpak	CDB-453 HQ	Polyhydroxymethacrylate	beta-Cyclodextrin derivertive
	ORpak	CDC-453 HQ	Polyhydroxymethacrylate	gamma-Cyclodextrin derivertive
	ORpak	CDBS-453	Silica	beta-Cyclodextrin derivertive
	ORpak	CRX-853	Polyhydroxymethacrylate	L-Amino acid derivative
Analysis of Saccharides	SUGAR	SH	Styrene divinylbenzene copolymer	Sulfo
	SUGAR	SC	Styrene divinylbenzene copolymer	Sulfo(Ca2+)
	SUGAR	SP0810	Styrene divinylbenzene copolymer	Sulfo(Pb2+)
	SUGAR	SZ5532	Styrene divinylbenzene copolymer	Sulfo(Zn2+)
	SUGAR	KS-800	Styrene divinylbenzene copolymer	Sulfo(Na+)
	USPpak	MN-431	Styrene divinylbenzene copolymer	Sulfo(Ca2+)
	EP	SC1011-7F	Styrene divinylbenzene copolymer	Sulfo(Ca2+)
Analysisn of Organic acids and Amino acids	RSpak	KC-811	Styrene divinylbenzene copolymer	Sulfo
	CXpak	P-421S	Styrene divinylbenzene copolymer	Sulfo(Na+)
Aqueous SEC (GFC)		SB400	Polyhydroxymethacrylate	-
	OHpak	SB-800 HQ	Polyhydroxymethacrylate	-
		KW400	Silica	Hydrophilic polymer
	PROTEIN	KW-800	Silica	Hydrophilic polymer
Multimode	RSpak	NN	Polyvinyl alcohol	Sulfo
	RSpak	JJ-50	Polyvinyl alcohol	Quaternary ammonium
	Asahipak	GS-HQ	Polyvinyl alcohol	-
	Asahipak	GS	Polyvinyl alcohol	-
Aqueous/Organic SEC	Asahipak	GF-HQ	Polyvinyl alcohol	-
Organic SEC (GPC)	GPC	KF-800 KF-400HQ KF-600	Styrene divinylbenzene copolymer	-
	GPC	K-800	Styrene divinylbenzene copolymer	-
	GPC	KD-800	Styrene divinylbenzene copolymer	-
	GPC	LF-804 LF-404 LF-604	Styrene divinylbenzene copolymer	-
	GPC	HFIP-800 HFIP-600	Styrene divinylbenzene copolymer	-
	GPC	HT-800	Styrene divinylbenzene copolymer	-
	GPC	UT-800	Styrene divinylbenzene copolymer	-
	GPC	AT-806S	Styrene divinylbenzene copolymer	-
Affinity	AFpak	32 kinds	Polyhydroxymethacrylate	32 kinds of ligand
Column switching	MSpak	PK	Hydrophilic copolymers containing N-vinyl acetamide	-
	MSpak	GF-310 GS-320	Polyvinyl alcohol	-
	Asahipak	ODP-51	Polyvinyl alcohol	Octadecyl
	MSpak	SP-80	Polyhydroxymethacrylate	Sulfopropyl
GPC clean-up	CLNpak	EV	Styrene divinylbenzene copolymer	-
	CLNpak	PAE	Polyvinyl alcohol	-

Caution:	In replacing water with, for instance, chloroform, which is not soluble in water, first replace the water with acetone and then replace acetone with chloroform.
Caution:	In replacing a solvent containing a salt with a solvent containing an organic solvent, use intermediate solvents, first water and next acetone, and, in replacing a solvent containing an organic solvent with a solvent containing a salt, first acetone and next water.
Caution:	If the liquid chromatograph is equipped with a device in which complete replacement of the solvent is not possible, e.g., a Bourdon pressure gauge, disassemble the device and wash it with the solvent that is to be used as the eluent.

2) When a column is stored improperly or for a long time, there may be the air at the inlet or outlet of the column. In such case, prior to connection of the column to the liquid chromatograph, remove the air from the column.

Caution:	Do not heat a column too rapidly or to the temperature higher than the maximum of the usable temperature.
Note:	Remove the cap of column inlet, and heat the column (usually warming by hands is enough) until in-column solvent comes out from the inlet endfitting. Then, connect the column to the liquid chromatograph, remeve the cap of column outlet, start the pump and check that the in-column solvent comes out from the outlet endfitting. The air in the column can be completely eliminated by this procedure.

3) Connect the column to the chromatograph so that the arrow on the plate will face downstream.

4) When the column is used at elevated temperature, heat the column to the desired temperature flowing the eluent at slow flow rate (0.2 to 0.3mL/min) and after reaching desired temperature, gradually increase the flow rate to the flow rate to be used.

5) Some columns need time for stabilization, and, the first measurement after connecting it to the chromatograph may be not so good.

3. Dismounting and safekeeping

1) After completing analysis, keep pumping eluent into the column at a flow rate of 0.2mL/min until the column is cooled down to room temperature.

2) After the column is cooled down to room temperature, dissconnect the column from the chromatograph and cap both ends of the column to prevent the eluent from leaking out.

Caution:	When the in-column solvent is different from the shipping-solvent from the manufacturer, replace the solvent before dismounting.
Caution:	Cap both ends of the column tightly so that the in-column solvent may not leak out.
Note:	The room temperature mentioned above means the temperature in the room where the column is stored.

3) Pack the column in the same way as when it was delivered from the manufacturer and store it in a room where the temperature fluctuates little (preferably, in a constant temperature room).

4) When GPC and GFC columns are stored for a long period of time, it is recommended to store in wet. Connect a Teflon tube of 1/16 inch in outside diameter, 0.8mm in inside diameter and 500mm in length to the column outlet.
Start pumping the eluent at a flow rate of 0.5mL/min and stop the pump as soon as it begins to flow out from the free end of the tube. Put 80mL of toluene or water into a 100mL bottle and soak the free end in the toluene or water to prevent air from entering the column. Dismount the column from the chromatograph, cap the column's inlet end and store it in a room where the temperature fluctuate little.



The columns to be stored in wet and the solvents to be used are as follows:
(a) The columns to be soaked in toluene:
GPC KF, K, KD, AT, HT, UT and HFIP.
(b) The columns to be soaked in purified water (containing sodium azide).
OHpak SB-800 HQ and PRTEIN KW series.

Note:	The solvent in the bottle should be replaced once a month with a fresh solvent to maintain the column performance as designated for a long period of time.

5) Stop the pump and leave the column on the chromatograph, if it is to be reused within three days.

Caution:	In case of one or more days suspension of chromatograph in which a solvent containing salt was used as eluent, replace the eluent with purified water, setting the flow rate at 0.5mL/min.

4. Use of guard column

1) Install a guard column immediately upstream of the main column to protect it from cotamination by the sample.

Note:	The guard column is intended to maintain the column performance as designated for a long period of time and not to improve its resolution.

2) Replace the gurad column at regular intervals. The intervals are different according to sample property and injection volume.

5. Sample pretreatment

1) Dissolve the sample in the solvent that is to be used as the eluent.

Note:	When the eluent is not stable, dissolve the sample in the eluent taken just before the injection. This procedure is important to have a smaller blank peak when RI detector is used.
Caution:	When using gradient elution, dissolve the sample in the starting eluent.
Caution:	Do not inject samples which are insoluble in the eluent.

2) Remove extraneous substances and insolubles from the dissolved sample by passing it through a 0.45micron filter. Use of disposable filter is recommended.

3) The use of SPE filter is also useful to remove the extraneous substances in the sample.

6. Eluent

1) Remove extraneous substances and insolubles from the eluent by passing it through a 0.45micron filter. Use of disposable filter is recommended.

2) Throughly degass the eluent. Apply ultrasonic vivration and degas decreasing the pressure using aspirator. When degassing of the eluent is insufficient, stable baseline cannot be obtained. Degassing is especially important when RI detector is used for the detection.
The use of Degasser (eluent degassing device), ERC-3000-alpha is recommended.

Caution:	The degassing is important when the column is used at elevated temperature to prevent air bubble at inside and outlet of the column.

7. Column testing method

The plate number in the inspection sheet attached to the column was calculated by the following formula:



The testing conditions to obtain the plate number is shown in Column Testing Conditions.
The chart speed should be 1.5 to 2.0 cm/min. The sensitivity of the detector should be adjusted to obtain peak height of about 10cm.

8. Other remark

Caution:	Do not make an impact on the column because the packing condition in the column may be disturbed the the impact. For example, do not drop a column from a high place.
Caution:	Do not bend the column or cause impact on it.
Caution:	Under no circumstances should the end-fittings of the column be removed as doing so will cause deterioration of the column performance.
Caution:	Do not change the column pressure or flow rate abruptly when a liquid chromatograoh is in use. Use a damper-equipped or pulseless pump to maintain the performance of the column at the designated level for a long period of time.
Caution:	Do not apply the pressure heigher than 20MPa, or the column material of stainless steel may be dtsfigured.

9. Warranty

1) Showa Denko shall replace any Shodex column,
(a) If found damaged at the time of delivery.
(b) If the plate number obtained by the purchaser as per the operation manual is significantly smaller than the one given in the inspection sheet attached to the column. Claims must be filed with Showa Denko within 10 days following delivery.

2) The following shall not be subject to warranty.
(a) Service life
(b) Deterioration of column performance resulting from improper handling.

Category	Column name		Coulmn cleaning procedure
Normal & Reversed Phase (Polymer- based)	Asahipak	NH2P	Reverse the column on chromatograph and flow 5mL of H2O, 60mL of 0.1M HClO4 aq., 5mL of H2O, 60mL of 0.1M NaOH aqueous solution and then 5mL of H2O at the flow rate of 0.5mL/min.
	Asahipak	ODP C8P, C4P	Flow 50mL of dioxiane, CH3CN or methanol.
	RSpak	RP18, DS
	RSpak	DE, GOLF CARB
	RSpak	DM	Flow 50mL of methanol, CH3CN or H2O.
	RSpak	DC	Flow 50mL of CH3CN, or 5mM NaOH aqueous solution. Or, inject 20 to 50 micro-L of 0.5M NaOH aqueous solution.
	RSpak	NN	Inject 20 to 50 micro-L of 2N nitric acid aqueous solution two or three times.
Normal & Reversed Phase (Silica- based)	ODSpak	F	Flow 50mL of dioxiane, THF, CH3CN or methanol.
	Silica	C18M, C18P
	Silicapak	E	Flow 50mL each of n-hexane, dichloroehtane, methanol, dichloroethane and n-hexane.
	SIlica	5SIL
	Silica	5C8, 5C4 5TMS, 5CN	Flow 50mL of dioxane, CH3CN or methanol.
	Silica	5NH	There is no appropriate washing method.
	Silica	5NPE
	Silica	5PYE
Ion excahnge	AXpak	WA	Inject 1 to 2 mL of 0.1M NaOH aqueous solution or 30% acetic acid aqueous solution. Connect the column in the reverse way and flow the eluent at 0.5mL/min.
	CXpak	P
	IEC	QA, DEAE SP, CM
	IEC	DEAE-420N SP-420N	Inject 500 micro-L of 0.1M NaOH aqueous solution, 50% formic acid aqueous solution, 20% CH3CN aqueous solution or 20% dimethyl sulfoxide aqueous solution several times. Flow the eluent at 0.3 to 0.5mL/min.
	Asahipak	ES-502N	Flow aqueous solution of high salt concentartion or 10mM NaOH aqueous solution for ionic adsorption and 50mM buffer solution containing 50% CH3CN or methanol for hydrophobic adsorption.
	Asahipak	ES-502C	Flow aqueous solution of high salt concentartion or 0.1N acetic acid aqueous solution for ionic adsorption and 50mM buffer solution containing 50% CH3CN aqueous solution or methanol for hydrophobic adsorption.
Hydrophobic chromato graphy	HIC	PH	Reverse the column on the HPLC system, flow the eluent at 0.5mL/min and inject 1 to 2 mL of 0.1M NaOH or 30% acetic acid aqueous solution several times.
Affinity chromato graphy	AFpak	31 kinds	Reverse the column on the HPLC system, flow an appropriate eluent at 0.5mL/min.
Sugar analysis	SUGAR	SH	Flow 50mL of 25mM H2SO4 aqueous solution at 50deg-C, 0.5mL/min.
	SUGAR	SC	( for SC1011, 1821) Flow 50mL of 0.1M Ca(NO3)2 aqueous solution at 50deg-C, 0.5mL/min. (for SC1211) After replacing in-column solvent completely with H2O, flow 30mL of 0.1M Ca(NO3)2 aqueous solution at 50deg-C, 0.5mL/min. Then, replace the in-column solvent completely with H2O and gradually increase the organic solvent concentration to the concentration to be used.
	SUGAR	SP	Flow 50mL of 0.2M Pb(NO3)2 at 50deg-C, 0.5mL/min.
	SUGAR	SZ	After replacing in-column solvent completely with H2O, flow 30mL of 0.1M Zn(NO3)2 aqueous solution at 50deg-C, 0.5mL/min. Then, replace the in-column solvent completely with H2O and gradually increase the organic solvent concentration to the concentration to be used.
	SUGAR	KS	Flow 10mM NaOH aqueous solution at 0.5mL/min. After flowing the solvent, replace the in-column solvent completely with H2O. (Sometimes, injection of 40micro-L of 0.1M NaOH aqueous solution is will be sufficient.)
	USPpak	MN	Flow 50mL of 0.1M Ca(NO3)2 aqueous solution at 50deg-C, 0.5mL/min.
Organic acid analysis	RSpak	KC	flow 50mL of 25mM H2SO4 aqueous solution at 50deg-C, 0.5mL/min.
Chiral separation	ORpak	CDA, CDB CDC	There is no appropreate cleaning method.
	ORpak	CDBS, CRX	There is no appropreate cleaning method.
GFC (aqueous GPC)	OHpak	SB	Reverse the column on the HPLC system, flow the eluent at 80% of ordinary flow rate. This is effective for the foreign substances remaining in the column, however not effective for the substances adsorbed to the packing material.
	PROTEIN	KW-800
		KW400-4F
Multimode	Asahipak	GS
Multisolvent GPC	Asahipak	GF
Organic GPC	GPC	KF, K, KD, LF HFIP, HT UT, AT
Ion chromato graphy	IC	I-524A	--Pollution by accumulation of metals-- 30minutes: 0.01M tartaric acid (pH3 to 4, adjust with 0.1M NaOH), 1.0mL/min --Pollution by proteins-- 150 minutes: 20% acetic acid aq., 0.2mL/min 150 minutes: water, 0.2mL/min --Pollution by organics-- 150 minutes: 20% acetonitrile/water, 0.2mL/min 150 minutes: water, 0.2mL/min
	IC	NI-424	Flow the mixture of eluent/methanol=90/10 at 0.6mL/min for two hours.
	IC	SI-90	--Pollution by low valency hydrophilic ions-- 1. 15 minutes: deionized water 2. 60 minutes: 10 times concentrated eluent 3. 15 minutes: deionized water 4. 60 minutes: eluent --Pollution by high valency hydrophobic ions-- 1. 15 minutes : deionized water 2. 10 minutes: 5% acetonitrile aqueous solution 3. 60 minutes: 100% acetonitrile 4. 30 minutes: deionized water 5. 60 minutes: eluent
	IC	SI-50	--Pollution by low valency hydrophilic ions-- (flow rate 0.3mL/min) 1. 25 minutes : deionized water 2. 100 minutes : 10 times concentrated eluent 3. 25 minutes : deionized water 4. 100 minutes : eluent --Pollution by high valency hydrophobic ions-- (flow rate 0.3mL/min) 1. 25 minutes : deionized water 2. 20 minutes : 5% acetonitrile aqueous solution 3. 100 minutes : 100% acetonitrile 4. 50 minutes : deionized water 5. 100 minutes : eluent
	IC	T	Flow 1M NaOH aqueous solution at 0.5mL/min for two hours.
	IC	R
	IC	Y	Inject 100micro-L of 1M HNO3 aqueous solution several times.
	IC	YK	Flow 50mL of 50mM tartaric acid aqueous solution or 50mM tartaric acid aqueous solution/CH3CN=50/50 at 0.3mL/min.
LC/MS, rapid analysis of medicines	Asahipak	ODP	Flow 50mL of dioxane, CH3CN or ethanol.
	MSpak	GF, GS	Reverse the column on the HPLC system, flow the eluent at 80% of ordinary flow rate. This is effective for the foreign substances remaining in the column, however not effective for the substances adsorbed to the packing material.
	MSpak	SP	Flow the eluent at 0.3 to 0.5mL/min and inject 500micro-L of 0.1M NaOH aqueous solution, 50% formic acid aqueous solution, 20% CH3CN aqueous solution or 20% dimethylsulfoxide aqueous solution several times.
GPC clean-up	CLNpak	EV	Reverse the column on the HPLC system, flow the eluent at 80% of ordinary flow rate. This is effective for the foreign substances remaining in the column, however not effective for the substances adsorbed to the packing material.

Category	Column type		Test conditions (1)Sample (2)Injection volume (3)Eluent (4)Flow rate (5)Temperature (6)Detector
Normal & reversed phase (Polymer- based)	Asahipak	ODP C8P,C4P	(1)0.75micro-L/mL n-Hexyl benzoate (2) 6micro-L(ODP), 10micro-L(C8P,C4P) (3) CH3CN/H2O=65/35 (4)0.6mL/min (5)30deg-C (6)UV(254nm)
	Asahipak	NH2P	(1)5mg/mL Sucrose (2)5micro-L(4E, 4D), 2micro-L(2D)(3) CH3CN/H2O=75/25 (4)0.6mL/min(4D), 1.0mL/min(4E), 0.2mL/min(2D) (5)30deg-C (6)RI
	RSpak	RP18-415	(1)1% Acetone (2)3micro-L (3) CH3CN/H2O=95/5 (4)1.0mL/min (5)RT(25deg-C) (6)UV(254nm)
	RSpak	RP18-613 RP18-413 DS-413	(1)1% 3-Pentanone (2)10micro-L(613), 5micro-L(413) (3) CH3CN/THF/H2O=30/30/40 (4)0.8mL/min(613), 0.6mL/min(413) (5)RT(25deg-C) (6)UV(262nm)
	RSpak	DS-613	(1)0.5% Benzene (2)5micro-L (3) CH3CN/THF/H2O=40/30/30 (4)0.8mL/min (5)RT(25deg-C) (6)UV(254nm)
	RSpak	DE-613	(1)0.2% Ethylene glycol (2)20micro-L (3) H2O (4)0.8mL/min (5)RT(25deg-C) (6)RI
	RSpak	DE-413 GOLF CARB	(1)0.6% Di-n-butyl ketone (2)10micro-L (3) CH3CN/H2O=50/50 (4)1.0mL/min (5)40deg-C (6)UV (262nm)
	RSpak	DE-213	(1)0.6% Di-n-butyl ketone (2)3micro-L (3) CH3CN/H2O=50/50 (4)0.2mL/min (5)40deg-C (6)UV (262nm)
	RSpak	DM-614	(1)2% Oxalic acid (2)5micro-L (3)0.005M H3PO4 aq. (4)0.8mL/min (5)RT(25deg-C) (6)RI
	RSpak	DC-613	(1)2% Sucrose (2)10micro-L (3)CH3CN/H2O=70/30 (4)0.8mL/min (5)RT(25deg-C) (6)RI
	RSpak	NN	(1)0.1% Uracil(414), 0.01% Uracil(614, 814) (2) 7micro-L(414, 614), 10micro-L(814) (3)0.1M NaH2PO3aq.(pH3.0 adjusted with phosphoric acid) (4)0.3mL/min(414), 0.8mL/min(614), 1.0mL/min(814) (5)35deg-C (6)RI(414), UV(260nm)(614, 814)
Normal & reversed phase (Silica- based)	ODSpak	F	(1)0.15micro-L/mL t-Butyl benzene (2)2micro-L(411/S), 20micro-L(411), 30micro-L(511) (3)Methanol/H2O=80/20 (4)1.0mL/min (5)RT(25deg-C) (6)UV(254nm)
	Silica	C18M C18P	(1)0.61mg/mL Naphthalene (2)2micro-L(4E), 1.5micro-L(4D) (3)Methanol/H2O=70/30 (4)1.0mL/min (5)30deg-C (6)UV(254nm)
	Silicapak	E-411	(1)0.05% p-Nitroaniline (2)5micro-L (3) IPA/Dichloromethane/n-hexane=1/50/49 (4)1.0mL/min (5)RT(25deg-C) (6)UV(254nm)
	Silica	5SIL
	Silica	5C8,5C4 5TMS,5CN	(1)0.61mg/mL Naphthalene (2)2micro-L(4E), 1.5micro-L(4D) (3)Methanol/H2O=55/45(5C4), Methanol/H2O=66/36(5C8), Methanol/H2O=50/50(TMS), Methanol/H2O=40/60(CN) (4)1.0mL/min (5)30deg-C (6)UV(254nm)
	Silica	5NH	(1)p-Nitrophenyl-alpha-D-glucopyranoside (2)2micro-L(4E), 1.5micro-L(4D) (3)CH3CN/H2O=95/5 (4)1.0mL/min (5)30deg-C (6)UV(254nm)
	Silica	5NPE
	Silica	5PYE
Ion exchange	AXpak	WA-624	(1)0.03% Uridine 5-monophosphate(UMP) (2)10micro-L (3)0.1M NaH2PO3(pH3.0 adjusted with phosphoric acid)/CH3CN=80/20 (4)1.0mL/min (5)RT(25deg-C) (6)UV(260nm)
	CXpak	P-421S	(1)1% Ethylene glycol (2)10micro-L (3)H2O (4)0.5mL/min (5)RT(25deg-C) (6)RI
	IEC	QA,DEAE SP,CM	(1)0.5% Acetone (2)20micro-L (3)50mM Sodium sulfate aq. (4)1.0mL/min (5)RT(25deg-C) (6)UV(280nm)
	IEC	DEAE-N	(1)Oligodeoxyadenylic acid (pd(A)7) (A) 0.01unit/2micro-L (2)2micro-L (3)50mM phosphoric acid buffer(pH6.0) + 0.17M NaCl + 1% Methanol (4)1.0mL/min (5)30deg-C (6)UV(260nm)
	IEC	SP-N	(1)0.2% Glycyl-L-tyrosine (2)1micro-L (3)20mM Acetate buffer(pH5.0) + 0.5M Sodium sulfate (4)1.0mL/min (5)RT(25deg-C) (6)UV(280nm)
	Asahipak	ES-502N	(1)0.025% Xanthine (2)15micro-L (3)50mM 1,3 Diaminopropane + 50mM NaCl(pH10.0) (4)1.0mL/min (5)30deg-C (6)UV detector(250nm)
	Asahipak	ES-502C	(1)0.025% Cytosine (2)15micro-L (3)100mM Sodium phosphate buffer(pH4.4) (4)1.0mL/min (5)30deg-C (6)UV(250nm)
Hydrophobic chromato- graphy	HIC	PH-814	(1)0.5% Acetone (2)20micro-L (3)H2O (4)1.0mL/min (5)RT(25deg-C) (6)UV(280nm)
Affinity chromato- graphy	AFpak	32 kinds
Sugar analysis	SUGAR	SH1011 SH1821	(1)1% Glycerol (2)10micro-L (3)0.005mM H2SO4 aq. (4)1.0mL/min (5)50deg-C (6)RI
	SUGAR	SP0810	(1)1% Glycerol (2)10micro-L (3)H2O (4)0.8mL/min (5)80deg-C (6)RI
	SUGAR	SC1011 SC1811	(1)1% Glycerol (2)10micro-L (3)H2O (4)1.0mL/min (5)80deg-C (6)RI
	SUGAR	SC1211	(1)1% Glycerol (2)10micro-L (3)CH3CN/H2O=25/75 (4)1.0mL/min (5)50deg-C (6)RI
	SUGAR	SZ5532	(1)1% Glucose (2)10micro-L (3)CH3CN/H2O=70/30 (4)1.0mL/min (5)50deg-C (6)RI
	SUGAR	KS-800	(1)3% Ethylene glycol(801), 2.5% Ethylene glycol(802), 0.5% Ethylene glycol(803), 0.8% Glucose(804 to 806), 0.5% Glucose(807) (2)5micro-L(801 to 802), 15micro-L(803), 20micro-L(804,805),10micro-L(806), 5micro-L(807) (3)H2O (4)1.0mL/min (5)50deg-C(801 to 806), RT(25deg-C)(807) (6)RI
	USPpak	MN	(1)0.5% Mannitol (2)10micro-L (3)H2O (4)1.0mL/min (5)60deg-C (6)RI
Organic acid analysis	RSpak	KC-811	(1)3% Acetic acid (2)5micro-L (3)0.1%H2SO4 aq. (4)1.0mL/min (5)40deg-C (6)RI
Chiral separation	ORpak	CDA-453	(1)0.1% 6-fluoro-DL-tryptophan (2)10micro-L (3)H2O (4)0.3mL/min (5)20deg-C (6)UV(254nm)
	ORpak	CDB-453	(1)0.1% DL-Methionine beta-naphthylamide (2)10micro-L (3)1.7% TEA/Acetic acid(pH4.0) + 0.1M NaCl in 20% CH3CN + 80% H2O (4)0.2mL/min (5)20deg-C (6)UV(254nm)
	ORpak	CDC-453	(1)0.02% Dancyl-DL-leucine (2)20micro-L (3)1% Acetic acid + 0.2M NaCl in10% CH3CN + 90% H2O (4)0.3mL/min (5)RT(25deg-C) (6)UV(254nm)
	ORpak	CDBS	(1)0.1% Nefopam-HCl (Sigma N-0391) (2)10micro-L (3)CH3CN/(1% Acetic acid + 0.2M NaCl) =30/70 (4)0.5mL/min (5)RT(25deg-C) (6)UV(254nm)
	ORpak	CRX-853	(1)0.1% R,S-Mandelic acid (2)20micro-L (3)0.25mM Copper sulfate aq. (4)1.0mL/min (5)RT(25deg-C) (6)UV(280nm)
GFC (Aqueous GPC)	OHpak	SB-800 HQ	(1)1% Ethylene glycol(802), 0.5% Ethylene glycol(802.5 to 806) (2)10micro-L(802), 15micro-L(802.5 to 806) (3)H2O (4)1.0mL/min (5)RT(25deg-C) (6)RI
	PROTEIN	KW-800	(1)0.2% Ethylene glycol (2)40micro-L (3)H2O (4)1.0mL/min (5)RT(25deg-C) (6)RI
Multimode	Asahipak	GS-HQ	(1)1% Ethylene glycol (2)30micro-L (3)H2O (4)0.6mL/min (5)30deg-C (6)RI
Multisolvent GPC	Asahipak	GF-HQ
Organic GPC	GPC	KF-800	(1)0.1% Propylbenzene(801 to 803), 0.05% Propylbenzene(804 to 807) (2)20micro-L(801 to 803), 40micro-L(804 to 807) (3)THF (4)1.0mL/min (5)RT(25deg-C) (6)UV(254nm)
	GPC	KF-600	(1)0.5% Propylbenzene(601), 0.05% Phthalic acid dicyclohexyl ester(602 to 604), 0.1% Phthalic acid dicyclohexyl ester(605 to 606) (2)5micro-L (3)THF (4)0.5mL/min (5)RT(25deg-C) (6)UV(254nm)
	GPC	KF-400HQ	(1)0.5% Propylbenzene(401402 to 406L) (2)5micro-L (3)THF (4)0.3mL/min (5)RT(25deg-C) (6)UV(254nm)
	GPC	LF	(1)0.1% Propylbenzene(804), 0.1% Phthalic acid dicyclohexyl ester(604, 404) (2)20micro-L(804), 5micro-L(604, 404) (3)THF (4)1.0mL/min(804), 0.5mL/min(604), 0.3mL/min(404) (5)RT(25deg-C) (6)UV(254nm)
	GPC	K-800	(1)0.1% Propylbenzene(801 to 803), 0.05% Propylbenzene(804 to 807) (2)20micro-L(801 to 803), 40micro-L(804 to 807) (3)THF (4)1.0mL/min (5)RT(25deg-C) (6)UV(254nm)
	GPC	KD-800	(1)1.0 % Acetone(801 to 804), 0.5 % Acetone(805 to 807)(2)20micro-L(801 to 804), 30micro-L(805,806), 40micro-L(807) (3)DMF (4)1.0mL/min (5)RT(25deg-C) (6)RI
	GPC	HFIP-800	(1)0.5% Acetone (2)30micro-L (3)HFIP (4)1.0mL/min (5)RT(25deg-C) (6)UV(280nm)
	GPC	HT, UT	(1)10% Monochlorobenzene toluene solution (2)10micro-L (3)Toluene (4)1.0mL/min (5)RT(25deg-C) (6)RI
	GPC	AT-806MS	(1)1% Acetone (2)20micro-L (3)Toluene (4)1.0mL/min (5)RT(25deg-C) (6)RI
Ion chroato- graphy	IC	I-524A	(1)10ppm NO3- (2)30micro-L (3)2.5mM Phthalic acid(pH4.0) (4)1.5mL/min (5)40deg-C (6)CD
	IC	NI-424	(1)10ppm SO42- (2)50micro-L (3)8mM p-Hydroxybenzoic acid + 2.8mM Bis(2hydroxyethyl)imino-tris(hydroxymethyl)methan + 2mM Phenylboronic acid + 5micro-M trans-1,2-Aminocyclohexane-N,N,N',N'-4 acetic acid aq.(4)1.0mL/min (5)40deg-C (6)CD
	IC	SI-90	(1)15ppm SO42- (2)20micro-L (3)1.8mM Na2CO3 + 1.7mM NaHCO3 aq. (4)1.0mL/min (5)RT(25deg-C) (6)Ssuppressed CD
	IC	SI-50	(1)10ppm Br- (2)20micro-L (3)3.2mM Na2CO3 + 1.0mM NaHCO3 aq. (4)0.7mL/min (5)RT(25deg-C) (6)Ssuppressed CD
	IC	SI-52	(1)40ppm SO4- (2)50micro-L (3)3.6mM Na2CO3 aq. (4)0.8mL/min (5)45deg-C (6)Ssuppressed CD
	IC	T-521	(1)20ppm Na+ (2)20micro-L (3)3mM Nitric acid aq. (4)1.0mL/min (5)40deg-C (6)CD
	IC	R	(1)1% Ethylene glycol (2)2micro-L (3)H2O (4)1.0mL/min (5)RT(25deg-C) (6)RI
	IC	Y-521	(1)20ppm Na+ (2)50micro-L (3)4mM Nitric acid aq. (4)1.5mL/min (5)40deg-C (6)CD
	IC	YK-421	(1)20ppm Ca2+ (2)15micro-L (3)5mM Tartaric acid + 1mM Dipicolinic acid + 24mM Boric acid aq.(4)1.0mL/min (5)40deg-C (6)CD

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