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Tilbage til startside for Thermo produkterThe SPE products enable rapid, economical sample clean-up prior to HPLC and GC analyses. This improves resolution and sensitivity, extends column lifetime and avoids instrument downtime. The range of consumables now features 11 phases in the HyperSep line, suitable for many applications and sample volumes in the environmental, chemical and food and beverage industries. Consistently high recoveries of the SPE range result in the phase (sorbent) retaining high levels of the analyte or compound of interest. Ordering information:
Aspire RP30 Desalting TipsThe Thermo Scientific Aspire RP30 Desalting Tip contains a bed of proprietary porous Styrene-divinylbenzene (SDVB) reversed-phase resin, which allows the removal of ion-suppressing contaminants from digested complex protein mixture prior to LC/MS or LC/MS/MS analysis, improving sensitivity and data quality (Table A). The Aspire tips offer superior overall peptide binding and recovery compared to the conventional C18 products (Figure 1). The Aspire tip employs a rapid purification protocol which streamlines low to medium-throughput sample clean-up. Aspire RP30 Highlights:
Table A. Results from 1-hour LC/MS/MS runs of tryptic-digested protein complex samples (mixture of 48 proteins). The Aspire tip further removes salts, detergents and other ion-suppressing interference present in the samples. As a result, peptide fragments are more readily detected and the total number of protein identifications increases.
HyperSep Aminopropyl
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| HyperSep Hypercarb SPE Products |
| Product# | Format | Bed Weight | Column Volume | Quantity |
| 60106-303 | SPE Columns | 50 mg | 1 mL | 50 |
| 60106-302 | SPE Columns | 100 mg | 1 mL | 30 |
| 60106-301 | SPE Columns | 200 mg | 3 mL | 30 |
| 60106-402 | SPE Columns | 500 mg | 6 mL | 20 |
| 60106-403 | SPE Columns | 1 g | 6 mL | 10 |
| 60106-404 | SPE Columns | 2 g | 15 mL | 10 |
| 60302-606 | 96-Well Plate | 10 mg | 1 mL | 1 |
| 60302-607 | 96-Well Plate | 25 mg | 1 mL | 1 |
| 60302-608 | 96-Well Plate | 50 mg | 1 mL | 1 |
| 60302-609 | 96-Well Plate | 100 mg | 1 mL | 1 |
| 60302-601 | Individual Wells | 10 mg | 1 mL | 100 |
| 60302-602 | Individual Wells | 25 mg | 1 mL | 100 |
| 60302-603 | Individual Wells | 50 mg | 1 mL | 100 |
| 60302-604 | Individual Wells | 100 mg | 1 mL | 100 |
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HyperSep™ Phenyl is commonly used for the enhanced retention of basic compounds. HyperSep Phenyl exhibits slightly different selectivity characteristics compared with other non-polar sorbents because of the presence of the aromatic ring.
- Commonly employed for non-polar interactions
- The electron density of the aromatic ring provides different selectivity than other non-polar sorbents
The HyperSep-96 system uses a 96-well plate format providing fast analysis without sacrificing accuracy or reproducibility. The design offers flexibility with individual removable wells for convenient method development.
Applications
- Extraction of aromatic compounds
- Extraction of basic analytes
Versatile polymeric material for retention of polar and non-polar analytes
• Exceptional recoveries for polar and non-polar analytes
• High and consistent recoveries
• High capacity material
• Fast and easy sample preparation method development
• pH stable 0-14
HyperSep Retain PEP (polar enhanced polymer) is a high purity, highly porous polystyrene DVB material modified with urea functional groups to give balanced retention of polar and non-polar analytes. The problems associated with column drying in traditional silica-based SPE materials are eliminated when using this product. This material is ideal for the retention of neutral compounds, or mixtures of compounds of different functionalities.
Applications
- Drugs and metabolites in biological fluids
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Peptides in serum, plasma or biological fluids
| HyperSep Retain PEP Polymeric SPE |
| Product# | Format | Bed Weight | Column Volume | Quantity | |
| 60107-201 | Column | 30mg | 1mL | 100 | |
| 60107-202 | Column | 30mg | 3mL | 50 |  |
| 60107-203 | Column | 60mg | 3mL | 50 | |
| 60107-204 | Column | 200mg | 3mL | 50 | |
| 60107-205 | Column | 500mg | 3mL | 50 | |
| 60107-206 | Column | 500mg | 6mL | 30 | |
| 60303-201 | Individual Wells | 5mg | 1mL | 100 | |
| 60303-202 | Individual Wells | 10mg | 1mL | 100 | |
| 60303-203 | Individual Wells | 30mg | 1mL | 100 | |
| 60303-204 | Individual Wells | 60mg | 1mL | 100 | |
| 60303-205 | 96-Well Plate | 5mg | 1mL | 1 | |
| 60303-206 | 96-Well Plate | 10mg | 1mL | 1 | |
| 60303-207 | 96-Well Plate | 30mg | 1mL | 1 | |
| 60303-208 | 96-Well Plate | 60mg | 1mL | 1 |
Polymeric material for the retention of acidic compounds
• Exceptional recoveries for acidic analytes
• High and consistent recoveries
• High capacity material
HyperSep™ Retain™-AX is a high purity, highly porous polystyrene DVB material partially modified with quaternary functional groups to retain acidic analytes. The problems associated with column drying in traditional silica-based SPE materials are eliminated when using this product.
Application
• Analysis of THC and its metabolites
| HyperSep Retain-AX Polymeric SPE |
| Product# | Format | Bed Weight | Column Volume | Quantity |
| 60107-401 | Column | 30mg | 1mL | 100 |
| 60107-402 | Column | 30mg | 3mL | 50 |
| 60107-403 | Column | 60mg | 3mL | 50 |
| 60107-404 | Column | 200mg | 3mL | 50 |
| 60107-405 | Column | 500mg | 3mL | 50 |
| 60107-406 | Column | 500mg | 6mL | 30 |
| 60303-401 | Individual Wells | 5mg | 1mL | 100 |
| 60303-402 | Individual Wells | 10mg | 1mL | 100 |
| 60303-403 | Individual Wells | 30mg | 1mL | 100 |
| 60303-404 | Individual Wells | 60mg | 1mL | 100 |
| 60303-405 | 96-Well Plate | 5mg | 1mL | 1 |
| 60303-406 | 96-Well Plate | 10mg | 1mL | 1 |
| 60303-407 | 96-Well Plate | 30mg | 1mL | 1 |
| 60303-408 | 96-Well Plate | 60mg | 1mL | 1 |
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Polymeric material for the retention of basic compounds
• Exceptional recoveries for basic analytes
• High and consistent recoveries
• High capacity material
HyperSep™ Retain™-CX is a high purity, highly porous polystyrene DVB material partially modified with sulfonic acid functional groups to retain basic analytes. The problems associated with column drying in traditional silica-based SPE materials are eliminated when using this product.
Application
• Analysis of a wide range of drugs of abuse including basic and neutral drugs
| HyperSep Retain-CX Polymeric SPE |
| Product# | Format | Bed Weight | Column Volume | Quantity |
| 60107-301 | Column | 30mg | 1mL | 100 |
| 60107-302 | Column | 30mg | 3mL | 50 |
| 60107-303 | Column | 60mg | 3mL | 50 |
| 60107-304 | Column | 200mg | 3mL | 50 |
| 60107-305 | Column | 500mg | 3mL | 50 |
| 60107-306 | Column | 500mg | 6mL | 30 |
| 60303-301 | Individual Wells | 5mg | 1mL | 100 |
| 60303-302 | Individual Wells | 10mg | 1mL | 100 |
| 60303-303 | Individual Wells | 30mg | 1mL | 100 |
| 60303-304 | Individual Wells | 60mg | 1mL | 100 |
| 60303-305 | 96-Well Plate | 5mg | 1mL | 1 |
| 60303-306 | 96-Well Plate | 10mg | 1mL | 1 |
| 60303-307 | 96-Well Plate | 30mg | 1mL | 1 |
| 60303-308 | 96-Well Plate | 60mg | 1mL | 1 |
HyperSep™SAX Strong Anion Exchanger is a strong anion exchange sorbent which offers minimal non-polar interactions. The decrease in these non-polar interactions results from the amine group that masks any effect of the carbon atoms in the functional group. The selectivity of HyperSep SAX can be modified by the addition of appropriate buffers, which increase or decrease this selectivity.
- Improved retention of weaker anions
- Extracts negatively-charged compounds from both aqueous and non-aqueous solutions
- Ideally suited for the extraction of weak acids, e.g. carboxylic acids
HyperSep SPE columns offer reproducible and reliable sample preparation in a traditional polypropylene column format. An even particle size distribution within the bed provides reproducible flow characteristics.
The HyperSep-96 system uses a 96-well plate format providing fast analysis without sacrificing accuracy or reproducibility. The design offers flexibility with individual removable wells for convenient method development.
Applications
- Isolation of anionic proteins, e.g. immunoglobulins and enzymes
- Removal of acidic pigments from food and beverage products
- Isolation of phenolic compounds
- Peptide pool fractionations
- Nucleic acids, nucleotides and surfactants
HyperSep™ SCX Strong Cation Exchanger is a strong cation exchanger which can be primarily used for the extraction of basic drugs. HyperSep SCX is manufactured using a trifunctional benzene sulphonic acid. The presence of the benzene ring means that HyperSep SCX can be used for non-polar interactions. This is due to the unequal distribution of electrons in the benzene ring. This dual mechanism of interaction is useful for analytes with both cationic and non-polar character.
- A strong cation exchange sorbent with a low pKa
- The benzene ring provides high potential for non-polar interactions
HyperSep SPE columns offer reproducible and reliable sample preparation in a traditional polypropylene column format. An even particle size distribution within the bed provides reproducible flow characteristics.
The HyperSep-96 system uses a 96-well plate format providing fast analysis without sacrificing accuracy or reproducibility. The design offers flexibility with individual removable wells for convenient method development.
Applications
- Extraction of antibiotics, drugs, organic bases, amino acids, catecholamines and herbicides
HyperSep™ Silica is generally regarded as the most polar sorbent available. It is primarily used for the retention of analytes from non-polar matrices. HyperSep Silica columns offer highly reproducible and reliable sample preparation in a simple format.
- Effectively separates compounds of very similar structure
- Adsorbs analytes from non-polar solvents such as hydrocarbons, chloro- or fluoro-substituted hydrocarbons or less polar esters and ethers
- Also used as a cation exchanger of intermediate strength in aqueous medium
HyperSep SPE columns offer reproducible and reliable sample preparation in a traditional polypropylene column format. An even particle size distribution within the bed provides reproducible flow characteristics.
The HyperSep-96 system uses a 96-well plate format providing fast analysis without sacrificing accuracy or reproducibility. The design offers flexibility with individual removable wells for convenient method development.
Applications
- Extraction of polar compounds such as alcohols, aldehydes, amines, drugs, metabolites, pesticides, herbicides and dyes
- Extraction of caroteniods, fat soluble vitamins, aflatoxins in food matrices
- Extraction of fatty acids and phospholipids from biological matrices
HyperSepTM SpinTip is a unique concept in solid phase extraction (SPE). HyperSep SpinTip is a pipette tip with a fine slit at the bottom (slit width 1- 2 µm) which permits liquid to pass through but retains the chromatographic material (20 - 30 µm) in the tip. This also eliminates the need for a filter and, thus, dead volume. HyperSep SpinTip contains just your desired chromatography material and nothing else and is excellent when working with small samples.
- Faster sample preparation with minimal sample loss
- No contamination from the supporting matrix
- Samples volumes as small as 0.1 µL can be handled
- Available in volumes of 0.1-10 µL and 10-200 µL
HyperSep SpinTip cartridges have been designed to overcome the problem of handling small volumes of sample. HyperSep SpinTip is available with a range of materials embedded in the tip wall to suit a wide range of applications.
Applications
- Desalting
- Protein purification
- MALDI
- Mass spectrometry
- Electrophoresis
- HPCE, HPLC and CEC
HyperSep Tip is a revolutionary patented micro pipette tip for sample preparation in which the chromatographic material is directly attached to the inner surface of the tip without using polymers or glue. This avoids potential problems with contamination or permeability. This also maximizes the surface area in contact with the sample.
- Revolutionary innovative SPE cartridge design
- Faster sample preparation
- Minimal sample loss
- No contamination from a supporting matrix
- Separation in volumes as low as 100 nanoliters
HyperSep Tip cartridges overcome the problem of handling small volumes of sample, for example the volumes associated with gel-isolated proteins used in proteolytic digests. HyperSep Tip is available with a range of materials embedded in the tip wall to suit a wide range of applications.
Applications
- Desalting
- Protein purification
- MALDI
- Mass spectrometry
- Electrophoresis
- HPCE, HPLC and CEC
HyperSepTM VerifyTM-AX offers retention through a combination of anion exchange and nonpolar interactions. It is an ideal choice for the extraction of acidic drugs and metabolites from biological matrices and is optimized for drugs such as THC. The HyperSep Verify-AX sorbent is a rigid, purified silica gel support to which the two functionalities are copolymerized.
- Mixed mode sorbent suited for the extraction of acidic drugs and metabolites from biological matrices
- Separation based on two functional groups: reversed phase C8 and an ion exchanger, quaternary amine
HyperSep SPE columns offer reproducible and reliable sample preparation in a traditional polypropylene column format. An even particle size distribution within the bed provides reproducible flow characteristics.
The HyperSep-96 system uses a 96-well plate format providing fast analysis without sacrificing accuracy or reproducibility. The desing offers flexibility, with individual removable wells for convenient method development.
Applications
- Analysis of THC and its metabolites
HyperSepTM VerifyTM-CX offers retention through a combination of ion exchange and non-polar interactions, making it an ideal choice for the extraction of basic drugs from biological matrices. The use of two functionalities for separation results in extremely clean extracts prior to analysis. HyperSep Verify-CX is copolymerized on a rigid, purified silica gel support.HyperSepTM VerifyTM-CX offers retention through a combination of ion exchange and non-polar interactions, making it an ideal choice for the extraction of basic drugs from biological matrices. The use of two functionalities for separation results in extremely clean extracts prior to analysis. HyperSep Verify-CX is copolymerized on a rigid, purified silica gel support.
- Mixed mode sorbent for extraction of basic drugs
- Separation based on two functional groups: reversed phase C8 and an ion exchanger, benzene sulfonic acid
- Ideal for extraction of drugs of abuse from biological matrices
HyperSep SPE columns offer reproducible and reliable sample preparation in a traditional polypropylene column format. An even particle size distribution within the bed provides reproducible flow characteristics.
The HyperSep-96 system uses a 96-well plate format providing fast analysis without sacrificing accuracy or reproducibility. The desing offers flexibility, with individual removable wells for convenient method development.
Applications
- Analysis of a wide range of drugs of abuse,including basic and neutral drugs
Quick, easy, cheap, effective, rugged and safe approach for determining pesticide residues in fruit, vegetables and other foods
- Determine greater number of pesticides than with standard SPE
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Easy to use
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Available in a number of configurations
The QuEChERS method is gaining in popularity around the world as the test of choice for multi-residue pesticide analysis in food and agricultural products. The QuEChERS method offers the advantages of high recoveries, accurate results and high sample throughput, low solvent and glassware usage, less labor and bench space, lower reagent costs. Yet the procedure is robust and rugged.
The QuEChERS method is a two-step process: extraction followed by clean up. The extraction step products use MgSO4 to reduce water in the sample, along with either NaCl (for most applications) or anhydrous sodium acetate for “base-sensitive” compounds (e.g., folpet or captan). The extraction step products are supplied in a 50 mL polypropylene centrifuge tube for convenient extractions.
The QuEChERS clean up step product line contain PSA (primary/secondary amine) for the removal of organic acids and polar pigments among other compounds. Some products couple the PSA with endcapped C18 for the removals of most lipids and sterols, or graphitized carbon black for the removal of sterols and pigments such as chlorophyll. A variety of tube formats and bed weights are available to accommodate large and small sample sizes.
| QuEChERS |
| Product# | Description | Quantity | |
| 60105-202 | QuEChERS Clean Up -150 mg Anhydrous Magnesium Sulfate, 50 mg PSA & 50 mg Carbon, 2 mL micro-centrifuge tubes | 100 |
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| 60105-203 | QuEChERS Clean Up -150 mg Anhydrous Magnesium Sulfate, 50 mg PSA, 2 mL micro-centrifuge tubes | 100 | |
| 60105-204 | QuEChERS Clean Up -150 mg Anhydrous Magnesium Sulfate, 50 mg PSA & 50 mg endcapped C18, 2 mL micro-centrifuge tubes | 100 | |
| 60105-205 | QuEChERS Clean Up -900 mg Anhydrous Magnesium Sulfate, 300 mg PSA & 150mg Carbon, 15 mL centrifuge tubes | 50 | |
| 60105-206 | QuEChERS Clean Up -900 mg Anhydrous Magnesium Sulfate, 300 mg PSA & 150 mg endcapped C18, 15 mL centrifuge tubes | 50 | |
| 60105-207 | QuEChERS Clean Up -400 mg PSA on bottom, 200 mg Graphitized Carbon on top, separated by a Teflon frit, 6 mL columns | 30 | |
| 60105-208 | QuEChERS Clean Up -250 mg Graphitized Carbon on top, 500 mg PSA on the bottom, separated by a Teflon frit, 6 mL columns | 30 | |
| 60105-209 | QuEChERS Clean Up -500 mg Graphitized Carbon on top, 500 mg PSA on bottom, separated by a Teflon frit, 6 mL columns | 30 | |
| 60105-210 | QuEChERS Extraction - 6 g Anhydrous Magnesium Sulfate, 1.5 g Anhydrous Sodium Acetate 50 mL polypropylene centrifuge tubes | 250 | |
| 60105-211 | QuEChERS Extraction - 4 g Anhydrous Magnesium Sulfate, 1 g NaCl, 50 mL polypropylene centrifuge tubes | 50 |
Thermo Scientific Online SPE Products have been designed to clean-up and concentrate target analytes in minutes. The products are available in different formats and utilize popular HyperSep SPE materials such as the HyperSep™ Retain polymeric range of materials and HyperSep Hypercarb™ for polar analyte retention.
Diagram: Load & elution positions for the HyperSep Online SPE setup
- Effective removal of contaminants such as proteins from samples
- Compatible with conventional HPLC systems such as the Thermo Scientific Surveyor
- Fast and effective clean-up and concentration of target compounds
Our range of Online SPE Products are available in three configurations:
- Thermo Scientific UNIGUARD Direct- Connection Guard Cartridge Holder and Cartridge
- Thermo Scientific Javelin Direct-Connection Guard Columns
- Thermo Scientific HPLC Column Hardware and Fittings
All products are available with the following HyperSep SPE chemistries:
- HyperSep Retain PEP – for retention of polar and non-polar analytes
- HyperSep Retain-CX – for retention of basic and non-polar analytes
- HyperSep Retain-AX – for retention of acidic and non-polar analytes
- HyperSep Hypercarb – for retention of extremely polar analytes
| Thermo Scientific HyperSep Online SPE |
| Product# | Description | |
| 60310-201 | HyperSep Retain PEP Javelin Direct-Connect Cartridge 2.1x10mm 4pk |
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| 60310-202 | HyperSep Retain PEP Javelin Direct-Connect Cartridge 3.0x10mm 4pk |
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| 60310-301 | HyperSep Retain-CX Javelin Direct-Connect Cartridge 2.1x10mm 4pk |
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| 60310-302 | HyperSep Retain-CX Javelin Direct-Connect Cartridge 3.0x10mm 4pk |
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| 60310-401 | HyperSep Retain-AX Javelin Direct-Connect Cartridge 2.1x10mm 4pk |
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| 60310-402 | HyperSep Retain-AX Javelin Direct-Connect Cartridge 3.0x10mm 4pk |
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| 60310-501 | HyperSep Hypercarb Javelin Direct-Connect Cartridge 2.1x10mm 4pk |
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| 60310-502 | HyperSep Hypercarb Javelin Direct-Connect Cartridge 3.0x10mm 4pk |
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| 60311-201 | HyperSep Retain PEP UNIGUARD Direct-Connect Cartridge 2.1x10mm 4pk |
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| 60311-202 | HyperSep Retain PEP UNIGUARD Direct-Connect Cartridge 3.0x10mm 4pk |
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| 60311-301 | HyperSep Retain-CX UNIGUARD Direct-Connect Cartridge 2.1x10mm 4pk |
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| 60311-302 | HyperSep Retain-CX UNIGUARD Direct-Connect Cartridge 3.0x10mm 4pk |
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| 60311-401 | HyperSep Retain-AX UNIGUARD Direct-Connect Cartridge 2.1x10mm 4pk |
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| 60311-402 | HyperSep Retain-AX UNIGUARD Direct-Connect Cartridge 3.0x10mm 4pk |
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| 60311-501 | HyperSep Hypercarb UNIGUARD Direct-Connect Cartridge 2.1x10mm 4pk |
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| 60311-502 | HyperSep Hypercarb UNIGUARD Direct-Connect Cartridge 3.0x10mm 4pk |
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| 60312-201 | HyperSep Retain PEP HPLC Column 2.1x20mm 1pk |
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| 60312-202 | HyperSep Retain PEP HPLC Column 3.0x20mm 1pk |
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| 60312-301 | HyperSep Retain-CX HPLC Column 2.1x20mm 1pk |
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| 60312-302 | HyperSep Retain-CX HPLC Column 3.0x20mm 1pk |
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| 60312-401 | HyperSep Retain-AX HPLC Column 2.1x20mm 1pk |
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| 60312-402 | HyperSep Retain-AX HPLC Column 3.0x20mm 1pk |
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| 60312-501 | HyperSep Hypercarb HPLC Column 2.1x20mm 1pk |
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| 60312-502 | HyperSep Hypercarb HPLC Column 3.0x20mm 1pk |
For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.