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HyPURITY C18 are ideal for chromatography of large peptides and small molecules Hypercarb capillary columns are for difficult-to-retain or resolve compounds A unique porous graphitic carbon HPLC phase for problem separations. BetaBasic® 18 columns are based on 150Å pore size, fully deactivated pure silica to ensure sharp peaks and excellent reproducibility. Their stable bonding chemistry and excellent surface coverage gives the columns exceptional low and high pH stability. BetaBasic 18 capillary columns are available in KAPPATM capillary columns are ideal for LC/MS. BetaBasic 18 capillary columns provide excellent chromatography for basic compounds at high pH, which allows for a wide choice of buffer selection in LC/MS applications.
o Based on ultra pure 190 Å pore size silica for analysis of small molecules to proteins HyPURITY C18 columns are based on the latest in silica technology - HyPURITY silica, which has less than 10ppm total metals and is exceptionally stable at low pH. The HyPURITY silica manufacturing process ensures reproducibility and quality. The homogeneous surface leads to uniform bonding coverage and eliminates silanol-analyte interactions that cause poor peak shapes for bases, acids and chelating compounds. HyPURITY C18 columns undergo forty separate quality control tests to meet stringent performance specifications. The 190Å pore size and excellent mass transfer properties of HyPURITY C18 makes it ideal for chromatography of large peptides, as well as small molecules, with unbeatable peak shape for all sample types. HyPURITY columns have proven performance for a wide range of applications, including small pharmaceuticals and peptides. Other column dimensions and hardware designs are available. Please contact us for more information if you do not see the exact column that you are interested in.
Due to the polar retention effect of Hypercarb, Hypercarb capillary columns provide excellent chromatography for difficult-to-retain polar compounds, or compounds that are difficult to resolve due to structural similarity. Hypercarb capillaries are available in two popular designs: KAPPA® and PIONEERTM direct-connection columns, which are both ideal for LC/MS. High efficiency KAPPA columns are available in 0.50mm, 0.32mm, and 0.18mm ID capillaries, and have an 8th inch stainless steel sleeve around the capillary to make them very robust for day-to-day laboratory use. They also have standard steel female endfittings, and so they can be used with standard column connectors. PIONEER capillary columns minimize band spreading and improve chromatographic efficiency by connecting directly into LC/MS and other detectors with a male 10-32 connection on one end. They are available in 0.50mm and 0.32mm ID. Due to the polar retnetion effect of Hypercarb, Hypercarb capillary columns provide excellent chromatography for difficult-to-retain polar compounds, or compounds that are difficult to resolve due to structural similarity. Hypercarb capillaries are ideal for nano and picomol concentration LC/MS applications.
o 100% porous graphitic carbon with very different selectivity to reversed-phase silica columns Hypercarb is a unique material for HPLC manufactured by Thermo Fisher Scientific Hypersil-Keystone. Hypercarb consists of fully porous spherical carbon particles comprised of flat sheets of hexagonally arranged carbon atoms. The carbon atoms have a fully satisfied valence and offer completely different retention and selectivity to silica and polymer based phases. Hypercarb can be used for RP and NP chromatography with a pH range from 1 to 14, and at extremes of temperature. Hypercarb excels in the analysis of structurally related components (e.g. stereo- and diastereoisomers and positional isomers) where no separation occurs on conventional materials. Hypercarb retains polar compounds via a 'polar retention effect' where a stronger analyte-sorbent attraction occurs as the polarity of the analyte increases, so the more polar the analyte the longer it's retention time compared to silica-based phases. Due to the unique retention mechanism of Hypercarb, we recommend that you do not use a column longer than 100mm or 150mm in length. Please contact us for technical assistance. Other column dimensions and hardware designs are available. Please contact us for more information if you do not see the exact column that you are interested in.
For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.
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MD Scientific ApS • Denmark • tlf. 7027 8565 • fax 7027 8566 • info@md-scientific.dk |
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