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Process Media Products
Affinity Chromatography
Toyopearl AF-Amino-650 media is a reactive resin used for the coupling of specific ligands for affinity chromatography. Ligands are immobilized by either peptide bond formation or reductive amination through their respective carboxylate or aldehyde groups.
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Structure:
Product Attributes:
| Pore size (mean): |
1,000Å |
| Particle size (mean): |
65µm (M-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer: |
20% ethanol |
| pH stability: |
2-13 |
| Shelf life: |
10 years |
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Literature:
Catalog -- Process Media Catalog, Affinity Chromatography
Product Overview -- Toyopearl- AF Amino-650M
Operating Conditions and Specifications
Instruction Manual -- Toyopearl AF-Amino-650M
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Ligand density |
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43411 |
Toyopearl AF-Amino-650M |
10mL |
70 - 130µeq/mL |
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08002 |
Toyopearl AF-Amino-650M |
25mL |
70 - 130µeq/mL |
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08039 |
Toyopearl AF-Amino-650M |
100mL |
70 - 130µeq/mL |
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18074 |
Toyopearl AF-Amino-650M |
1L |
70 - 130µeq/mL |
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18316 |
Toyopearl AF-Amino-650M |
5L |
70 - 130µeq/mL |
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Toyopearl AF-Blue HC-650 resin is functionalized with Cibachron Blue F3G-A. It has high dynamic binding capacity for proteins, particularly albumin, and has improved caustic stability and lower dye leakage than traditional agarose materials
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Structure:
Product Attributes:
| Pore size (mean): |
1,000Å |
| Particle size (mean): |
65µm (M-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer: |
20% ethanol |
| pH stability: |
4-9 |
| Shelf life: |
10 years (estimated) |
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Literature:
Catalog -- Process Media Catalog, Affinity Chromatography
Product Overview -- Toyopearl AF-Blue HC-650M, for one step Affinity Isolation of Kinases, Phosphates, Dehydrogenases, Albumin, Factor II, FactorIX, and Interferon
Operating Conditions and Specifications
Instruction Manual -- Toyopearl AF-Blue HC-650M
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Comparative information |
Comparison of human serum albumin binding capacity at various pH values for AF-Blue HC-650 and Agarose
Comparative dye leakage study of AF-Blue HC-650M and Agarose Blue @ 25°C (after 24 hours)
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Ligand density |
Adsorption capacity |
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19688 |
Toyopearl AF-Blue HC-650M |
25mL |
>16µmol/mL |
>18mg/mL* |
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19689 |
Toyopearl AF-Blue HC-650M |
100mL |
>16µmol/mL |
>18mg/mL* |
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19690 |
Toyopearl AF-Blue HC-650M |
1L |
>16µmol/mL |
>18mg/mL* |
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19691 |
Toyopearl AF-Blue HC-650M |
5L |
>16µmol/mL |
>18mg/mL* |
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Toyopearl AF-Carboxy-650 media is a reactive resin which couples to the amino groups of proteins or smaller molecular weight ligands. The carbodiimide mediated coupling reaction produces an amide bond between the support and the ligand.
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Structure:
Product Attributes:
| Pore size (mean): |
1,000Å |
| Particle size (mean): |
65µm (M-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer: |
20% ethanol |
| pH stability: |
2-13 |
| Shelf life: |
10 years |
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Literature:
Catalog -- Process Media Catalog, Affinity Chromatography
Operating Conditions and Specifications
Instruction Manual -- Toyopearl AF-Carboxy-650M
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Ligand density |
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43412 |
Toyopearl AF-Carboxy-650M |
10mL |
80 - 120µeq/mL |
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08006 |
Toyopearl AF-Carboxy-650M |
25mL |
80 - 120µeq/mL |
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08041 |
Toyopearl AF-Carboxy-650M |
100mL |
80 - 120µeq/mL |
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18827 |
Toyopearl AF-Carboxy-650M |
1L |
80 - 120µeq/mL |
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18828 |
Toyopearl AF-Carboxy-650M |
5L |
80 - 120µeq/mL |
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Toyopearl AF-Chelate-650 resin is derivatized with iminodiacetic acid for immobilized metal affinity chromatography or IMAC.IMAC works primarily by binding to histidine and free cysteine containing sequences of proteins or peptides.
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Structure:
Product Attributes:
| Pore size (mean): |
1,000Å |
| Particle size (mean): |
65µm (M-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer: |
20% ethanol |
| pH stability: |
2-12 |
| Shelf life: |
10 years |
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Literature:
Catalog -- Process Media Catalog, Affinity Chromatography
Operating Conditions and Specifications
Instruction Manual -- Toyopearl AF-Chelate-650M
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Ligand density |
Adsorption capacity |
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14475 |
Toyopearl AF-Chelate-650M |
25mL |
25-45µeq/mL |
>60mg/mL* |
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19800 |
Toyopearl AF-Chelate-650M |
100mL |
25-45µeq/mL |
>60mg/mL* |
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14907 |
Toyopearl AF-Chelate-650M |
1L |
25-45µeq/mL |
>60mg/mL* |
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14908 |
Toyopearl AF-Chelate-650M |
5L |
25-45µeq/mL |
>60mg/mL* |
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Toyopearl AF-Epoxy-650 media is an activated resin provided in dry form for the immobilization of protein ligands for affinity chromatography. It is used when high densities of low molecular weight molecules need to be attached. It is also useful when a conversion to other special functional groups is required prior to ligand immobilization. For instance, its hydrazide form is very useful for carbohydrates or glycoprotein ligands.
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Structure:
Product Attributes:
| Pore size (mean): |
1,000Å |
| Particle size (mean): |
65µm (M-grade) |
| Pressure rating: |
3 bar |
| Shipping condition: |
dry |
| Storage temperature: |
ambient |
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Literature:
Catalog -- Process Media Catalog, Affinity Chromatography
Operating Conditions and Specifications
Instruction Manual -- Toyopearl AF-Epoxy-650M
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin quantity |
Ligand density |
Adsorption capacity |
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43402 |
Toyopearl AF-Epoxy-650M |
5g* |
600 - 1000µeq/g |
>60mg/g** |
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08000 |
Toyopearl AF-Epoxy-650M |
10g* |
600 - 1000µeq/g |
>60mg/g** |
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08038 |
Toyopearl AF-Epoxy-650M |
100g* |
600 - 1000µeq/g |
>60mg/g** |
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18315 |
Toyopearl AF-Epoxy-650M |
1,000g* |
600 - 1000µeq/g |
>60mg/g** |
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Toyopearl AF-Formyl-650 media is a reactive resin which binds exclusively to primary amines. This forms a stable secondary amine linkage with the ligand used.
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Structure:
Product Attributes:
| Pore size (mean): |
1,000Å |
| Particle size (mean): |
65µm (M-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer: |
20% ethanol |
| pH stability: |
6-9 |
| Shelf life: |
10 years |
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Literature:
Catalog -- Process Media Catalog, Affinity Chromatography
Operating Conditions and Specifications
Instruction Manual -- Toyopearl AF-Formyl-650M
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Ligand density |
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43413 |
Toyopearl AF-Formyl-650M |
10mL |
40 - 70µeq/mL |
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08004 |
Toyopearl AF-Formyl-650M |
25mL |
40 - 70µeq/mL |
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08040 |
Toyopearl AF-Formyl-650M |
100mL |
40 - 70µeq/mL |
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17396 |
Toyopearl AF-Formyl-650M |
1L |
40 - 70µeq/mL |
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17397 |
Toyopearl AF-Formyl-650M |
5L |
40 - 70µeq/mL |
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Toyopearl AF-Heparin HC-650 media is a new high capacity affinity resin with an Antithrombin III (AT III) dynamic binding capacity of approximately 4 mg/mL. Due to its polyanionic nature heparin interacts with a wide range of biomolecules including plasma components, lipoprotein lipase, collagenase, and DNA polymerase |
Structure:
Product Attributes:
| Pore size (mean): |
1000Å |
| Particle size (mean): |
65µm (M-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer: |
20% ethanol |
| pH stability: |
5-10 |
| Shelf life: |
10 year (est.) |
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Literature:
Catalog -- Process Media Catalog, Affinity Chromatography
Operating Conditions and Specifications
Instruction Manual -- Toyopearl AF-Heparin-650M
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Comparative information |
Dynamic Binding Capacity
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Ligand density |
Adsorption capacity |
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20030 |
Toyopearl AF-Heparin HC-650M |
10mL |
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>5mg/mL* |
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20031 |
Toyopearl AF-Heparin HC-650M |
100mL |
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>5mg/mL* |
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20032 |
Toyopearl AF-Heparin HC-650M |
1L |
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>5mg/mL* |
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20033 |
Toyopearl AF-Heparin HC-650M |
5L |
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>5mg/mL* |
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Toyopearl AF-Red-650 resin is functionalized with Procion Red HE-3B (also known as Reactive Red 120). It is used for nucleotide-dependent enzymes, lipoproteins, plasminogen, peptides, hormones, and cytotoxins
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Structure:
Product Attributes:
| Pore size (mean): |
1,000Å |
| Particle size (mean): |
65µm (M-grade) |
| Pressure rating: |
45 psi |
| Shipping buffer: |
20% ethanol |
| pH stability: |
4-9 |
| Shelf life: |
10 years (estimated) |
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Literature:
Catalog -- Process Media Catalog, Affinity Chromatography
Operating Conditions and Specifications
Instruction Manual -- Toyopearl AF-Red-650M
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Container size |
Ligand density |
Adsorption capacity |
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08651 |
Toyopearl AF-Red-650M |
25mL |
7µmol/mL |
2.5 - 4.5mg/mL* |
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19801 |
Toyopearl AF-Red-650M |
100mL |
7µmol/mL |
2.5 - 4.5mg/mL* |
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42102 |
Toyopearl AF-Red-650M |
1L |
7µmol/mL |
2.5 - 4.5mg/mL* |
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Toyopearl AF-Tresyl-650 media is an activated resin which readily binds to amine and thiol groups. The resin is provided dry for introduction into a buffered solution containing protein or other ligand in neutral to slightly alkaline (pH 7-8) conditions.
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Structure:
Product Attributes:
| Pore size (mean): |
1,000Å |
| Particle size (mean): |
65µm (M-grade) |
| Pressure rating: |
45 psi |
| Shipping condition: |
dry |
| Storage temperature: |
4°C |
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Literature:
Catalog -- Process Media Catalog, Affinity Chromatography
Operating Conditions and Specifications
Instruction Manual -- Toyopearl AF-Tresyl-650M
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Container size |
Ligand density |
Adsorption capacity |
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14471 |
Toyopearl AF-Tresyl-650M |
5g* |
80µmol/mL |
>60mg/g** |
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14472 |
Toyopearl AF-Tresyl-650M |
100g* |
80µmol/mL |
>60mg/g** |
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14905 |
Toyopearl AF-Tresyl-650M |
200g* |
80µmol/mL |
>60mg/g** |
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14906 |
Toyopearl AF-Tresyl-650M |
1,000g* |
80µmol/mL |
>60mg/g** |
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18371 |
Toyopearl AF-Tresyl-650M |
5,000g* |
80µmol/mL |
>60mg/g** |
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Toyopearl AF-rProtein A-650F is a high capacity affinity resin designed for efficient capturing of monoclonal antibodies at production scale. The recombinant ligand is derived from one of the IgG binding domains of Protein A by optimizing amino acid sequence. It is expressed in E. coli, free of animal derived product. Multipoint attachment of this ligand to the rigid Toyopearl matrix reduces ligand leaching and enhances mechanical, thermal and alkali stability.
Typical static IgG binding capacity is > 45g/L and typical dynamic IgG binding capacity is > 30 g/L (at 2 minutes residence time, 1 g/L loading concentration ). Cleaning-in-place (CIP) is possible with 0.1 M and 0.5 M NaOH. After more than 100 CIP cycles with 0.1 M NaOH at 16 min contact time typically more than 90 % of initial dynamic binding capacity is retained.
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 Structure: |
HW65-rProtein A
Ligand: recombinant Protein a variant (E. coli)
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Product Attributes:
Pore size (mean): |
1,000 Å
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Particle size (mean): |
45 µm (F-grade)
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Pressure rating: |
3 bar
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Shipping buffer: |
20% ethanol
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SBC: |
> 45 g/L
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DBC: |
> 30 g/L
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Toyopearl AF-rProtein A-650F provides |
Efficient MAB capturing with high purity from high titer feedstocks
High IgG capacity over a wide range of velocities
High alkaline stability for effective cleaning and sanitization
Rigid polymethacrylate base matrix with ideal pressure flow characteristics
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| Binding capacity & Alkaline stability |
Binding capacity at 10 mg/mL
Dynamic IgG binding capacity at various residence times Breakthrough curves for IgG loading at a concentration of 10 mg/ml: typical DBC at 10% breakthrough for Toyopearl AF-rProtein A-605F (red): 33 mg/mL@ 60 cm/hr (5 min residence time) - 24 mg/mL @ 200 cm/hr (1.5 min residence time)
Alkaline stability 0.1/0.5 M NaOH
Chemical Stability of Toyopearl AF-rProtein A-650F IgG binding capacity is not significantly impaired by more than 100 CIP cycles with 0.1 M NaOH (red, 16 min contact time/cycle) and 0.5 M NaOH (grey, 17 min contact time/cycle). Dynamic binding capacity of hIgG determined at 10% breakthrough.
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
| P/N |
Description |
Resin volume |
Pore size
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Particle size
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| 22803 |
Toyopearl AF-rProtein-650F |
10 mL |
1,000 Å
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45 µm
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| 22804 |
Toyopearl AF-rProtein-650F |
25 mL |
1,000 Å
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45 µm |
| 22805 |
Toyopearl AF-rProtein-650F |
100 mL |
1,000 Å |
45 µm
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| 22806 |
Toyopearl AF-rProtein-650F |
1 L |
1,000 Å |
45 µm
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| 22807 |
Toyopearl AF-rProtein-650F |
5 L |
1,000 Å |
45 µm
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| 22808 |
Toyopearl AF-rProtein-650F |
50 L |
1,000 Å |
45 µm
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| 22809 |
ToyoScreen AF-rProtein-650F |
1 mL x 5 |
1,000 Å |
45 µm
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| 22810 |
ToyoScreen AF-rProtein-650F |
5 mL x 1 |
1,000 Å |
45 µm
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| 22811 |
ToyoScreen AF-rProtein-650F |
5 mL x 5 |
1,000 Å |
45 µm
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| 22815 |
ProteinA-R28 ELISA Kit |
Kit |
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For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.
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