Process Media Products - Resiner

Process Step

Particle Size

Process Media

Capture

100µm

Toyopearl SuperButyl-550C
Toyopearl Hexyl-650C
Toyopearl Butyl-650C
Toyopearl Phenyl-650C

65µm

Toyopearl Butyl-600M
Toyopearl Phenyl-600M
Toyopearl PPG-600M

Toyopearl Butyl-650M
Toyopearl Phenyl-650M
Toyopearl Ether-650M

35µm

Toyopearl Butyl-650S
Toyopearl Phenyl-650S
Toyopearl Ether-650S

30µm

TSKgel Phenyl-5PW (30)
TSKgel Ether-5PW (30)

20µm

TSKgel Phenyl-5PW (20)
TSKgel Ether-5PW (20)

10µm

Toyopearl Butyl-600 resin has the second highest hydrophobicity of the HIC ligands offered by TBL. Its pore size is between that of Toyopearl Butyl-650 and Toyopearl SuperButyl-550 media. It has been optimized for the purification of monoclonal antibodies and is typically used as an intermediate purification or polishing step.

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most) 

Product Attributes:

Literature:

Catalog - Process Media, Hydrophobic Interaction Chromatography

Product Overview -- Toyopearl Butyl-600M, for Hydrophobic Interaction Chromatography of monoclonal antibodies and hydrophilic proteins

Technical Presentation -- Purification of monoclonal antibodies using new pore size optimized HIC resins

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Toyopearl Butyl-650 resin has the second highest hydrophobicity of the HIC ligands offered by TBL. Its pore size is the largest of our three butyl resins. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in intermediate purification and polishing steps

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most) 

Product Attributes:

Literature:

Catalog - Process Media, Hydrophobic Interaction Chromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Toyopearl Ether-650 resin has the highest hydrophilicity of the HIC ligands offered by TBL. It is useful for very hydrophobic molecules such as membrane proteins. It is typically used in intermediate purification and polishing steps. For high performance separations, the use of TSKgel Ether-5PW media is suggested. Both products have the same selectivity.

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most) 

Product Attributes:

Literature:

Catalog - Process Media, Hydrophobic Interaction Chromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Toyopearl Hexyl-650 resin has the highest hydrophobicity of the HIC ligands offered by TBL. It is used for very hydrophilic proteins or where a low salt elution environment is required. It is useful as an early capture step or for intermediate purification.

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Product Attributes:

Literature:

Catalog - Process Media, Hydrophobic Interaction Chromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Toyopearl Phenyl-600 resin has the middle hydrophobicity of the five HIC ligands offered by TBL. Its pore size has been optimized for the chromatography of monoclonal antibodies and it is typically used in an intermediate or polishing step.

It is a very high recovery and high dynamic capacity resin for both large and small proteins. When used as the polishing step up to an extra 5% yield may be seen compared to other Phenyl resins.

To see other products optimized for HIC purification of mAbs please see Toyopearl PPG-600 and Toyopearl Butyl-600 medias.

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Product Attributes:

Literature:

Catalog - Process Media, Hydrophobic Interaction Chromatography

Product Overview -- Toyopearl Phenyl-600M for high capacity Hydrophobic Interaction Chromatography and high recovery of large and small proteins

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Toyopearl Phenyl-650 resin is the middle hydrophobicity of the five HIC ligands offered by TBL. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in intermediate purification and polishing steps. For high performance separations, the use of TSKgel Phenyl-5PW media is suggested. Both products have the same selectivity.

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Product Attributes:

Literature:

Catalog - Process Media, Hydrophobic Interaction Chromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Toyopearl PPG-600 (polypropylene glycol) resin has the second highest hydrophilicity of the HIC ligands offered by TBL. It has been optimized for the purification of monoclonal antibodies and is used as an intermediate purification or polishing step. It has extremely good capacity and recovery for mAbs.

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Product Attributes:

Literature:

Catalog - Process Media, Hydrophobic Interaction Chromatography

Product Overview -- Toyopearl PPG-600M, for Hydrophobic Interaction Chromatography of Monoclonal Antibodies, Hydrophobic Proteins and Pegylated Targets

Technical Presentation -- Purification of monoclonal antibodies using new pore size optimized HIC resins

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Toyopearl SuperButyl-550 resin has the second highest hydrophobicity of the HIC ligands offered by TBL. Its pore size is the smallest of our three butyl resins. Primary applications are for the separation of smaller proteins. Depending on the feedstock's molecular size profile, it is also useful in negative or flow through chromatography. It can be used for capture and intermediate purification process steps

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Product Attributes:

Literature:

Catalog - Process Media, Hydrophobic Interaction Chromatography

Product Overview -- Toyopearl Super Butyl-550C, for Hydrophobic Interaction Chromatography of Proteins and Peptides

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

TSKgel Ether-5PW resin is the highest hydrophilicity of the five HIC ligands offered by TBL. It is useful for the separation of very hydrophobic molecules such as membrane proteins.  It is typically used for high performance chromatography and polishing steps. It has the same selectivity as the larger particle Toyopearl Ether-650 products. It often is used instead of cross-linked dextrans with exclusion limits of 10,000 MW.

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Product Attributes:

Literature:

Catalog - Process Media, Hydrophobic Interaction Chromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

TSKgel Phenyl-5PW resin is the middle hydrophobicity of the five HIC ligands offered by TBL. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used for high performance chromatography and polishing steps. It has the same selectivity as the larger particle Toyopearl Phenyl-650 products.

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most) 

Product Attributes:

Literature:

Catalog - Process Media, Hydrophobic Interaction Chromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.