Ion Exchange SEC - Resiner

Toyopearl CM-650 resin is a weak cation exchanger for biomolecule purification.  It can be used for high throughput capture, intermediate purification, and polishing process steps.

Structure:

Product Attributes:

Literature:

Catalog -- Process Media, Ion Exchange Chromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Toyopearl DEAE-650 media is a weak anion exchanger for blood fractionation and other biomolecule purification. It can be used for high throughput capture, intermediate purification, and polishing process steps. If more powerful resolution is needed TSK-GEL DEAE-5PW resin should be used. Selectivity remains the same. 

Structure:

Product Attributes:

Literature:

Catalog -- Process Media, Ion Exchange Chromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Toyopearl GigaCap CM-650 media is a high capacity cation exchange resin* optimized specifically for high throughput purification of IgG. Toyopearl HW-65 resin is the polymeric base bead, which is chemically modified to provide a high level of cationic binding sites for IgG. It exhibits typical dynamic binding capacities in the vicinity of 100 mg/mL for IgG. This new polymer product is stable at high linear velocities, has good pressure flow characteristics and will withstand back pressures up to 3 bar. The new higher capacity and back pressure stability of Toyopearl GigaCap CM-650 resin creates opportunities for increased throughput in downstream cation exchange purification steps.

Toyopearl GigaCap CM-650 resin has a different selectivity than Toyopearl GigaCap S-650 resin. With its unusually high capacity for a weak cationic exchange resin this selectivity may be exploited for either mAb or traditional protein purifications.

Toyopearl GigaCap CM-650 media also has excellent base stability in 0.5mol/L NaOH.
*specifically designed for packed bed use 

Structure:

Product Attributes:

Literature:

Brochure -- Toyopearl GigaCap Ion Exchange Resins

Product Overview -- Toyopearl GigaCap CM-650M, for high capacity cation exchange chromatography of IgG

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Toyopearl GigaCap Q-650 media is a high capacity anion exchange resin* optimized for the capture and purification of both small and large proteins. Toyopearl HW-65 resin is the polymeric base bead chemically modified to provide a higher number of anionic binding sites for increased resin capacity . It exhibits typical dynamic binding capacities in the vicinity of 175mg/mL for BSA (66kD) and 49mg/mL for thyroglobulin (660kD). 

This new polymer product maintains high capacity at increased linear velocities, has good pressure flow characteristics and will withstand back pressures up to 3 bar. The new higher capacity and back pressure stability of Toyopearl GigaCap Q-650 resin  creates opportunities for increased throughput in all anion exchange purification steps.

In addition to the high DBC and good pressure flow characteristics, Toyopearl GigaCap Q-650 media displays excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step.

*specifically designed for packed bed use 

Structure:

Product Attributes:

Literature:

Brochure -- Toyopearl GigaCap Ion Exchange Resins

Product Overview -- Toyopearl GigaCapQ-650M for high capacity Anion Exchange Chromatography of small and large proteins

Technical Presentation -- Novel High Binding Capacity Ion Exchange Resins for Biochromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Toyopearl GigaCap S-650 media is a high capacity cation exchange resin* optimized specifically for the capture and purification of IgG. Toyopearl HW-65 resin is the polymeric base bead chemically modified to provide a high level of cationic binding sites for IgG. It exhibits typical dynamic binding capacities in the vicinity of 140 mg/mL for IgG. This new polymer product is stable at high linear velocities, has good pressure flow characteristics and will withstand back pressures up to 3 bar. The new higher capacity and back pressure stability of Toyopearl GigaCap S-650  resin creates opportunities for increased throughput in downstream cation exchange purification steps.

While Toyopearl GigaCap S-650 media was specifically optimized for IgG, it also exhibits high binding capacities for smaller proteins. Typical dynamic binding capacities for lysozyme are in excess of 160 mg/mL.

Toyopearl GigaCap S-650M media also has excellent base stability in 1.0mol/L NaOH and shows robust clean in place properties with minimal loss of capacity after 50 X 1 hour cycles of caustic exposure also using 1.0mol/L NaOH.

*specifically designed for packed bed use 

Structure:

Product Attributes:

Literature:

Application Note -- Toyopearl GigaCap S-650M, a very high capacity cation exchange resin

Brochure -- Toyopearl GigaCap Ion Exchange Resins

Journal Article -- Development of a High-Capacity MAb Capture Step Based on Cation-Exchange Chromatography   (BioProcess Int'l)

Product Overview -- Toyopearl GigaCap S-650M for High Capacity Cation Exchange Chromatography of IgG

Technical Presentation -- A High Capacity Strong Cation Exchange Resin for the Chromatographic Purification of Monoclonal Antibodies and Other Proteins

Technical Presentation -- Novel High Binding Capacity Ion Exchange Resins for Biochromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559s.

Toyopearl MegaCap® II SP-550EC resin is a large particle size strong cation exchanger useful for purification of molecules ranging in size from small peptides such as insulin to proteins up to 100kD.  It is used for high throughput capture of feedstocks.

Structure:

Product Attributes:

Literature:

Catalog -- Process Media, Ion Exchange Chromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Toyopearl Q-600C AR resin is a strong anion exchanger designed to be more alkaline resistant (AR) than Toyopearl QAE-550C resin. It has a slightly larger pore diameter than Toyopearl QAE-550C resin, but its ligand attachment chemistry creates significantly more dynamic binding capacity (up to 3x higher) than Toyopearl QAE-550C resin. (see comparative data) It tolerates caustic cleaning solutions up to 1.0 mol/L NaOH well with essentially no change in DBC.

Its elution kinetics are good and its retentivity is slightly stronger than Toyopearl QAE-550C resin.

Structure:

proprietary

Product Attributes: Pore size (mean): 750 Å Particle size (mean): 100 µm (C-grade) Pressure rating: 3 bar Shipping buffer: 20% ethanol pH stability: 2-13 Shelf life: 10 years

Literature:

Catalog -- Process Media, Ion Exchange Chromatography

Toyopearl QAE-550 resin is a strong anion exchanger useful for purification of proteins up to 100kD.  It is used for high throughput capture of feedstocks.

Structure:

Product Attributes:

Literature:

Catalog -- Process Media, Ion Exchange Chromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Toyopearl SP-550 resin is a strong cation exchanger useful for purification of molecules such as insulin and proteins up to 100kD.  It is used for high throughput capture of feedstocks.

Structure:

Product Attributes:

Literature:

Catalog -- Process Media, Ion Exchange Chromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Toyopearl SP-650 media is a strong cation exchanger for biomolecule purification.  It can be used for high throughput capture, intermediate purification, and polishing process steps. If more powerful resolution is needed the smaller particle TSKgel SP-5PW resin should be used. Selectivity remains the same.

Structure:

Product Attributes:

Literature:

Catalog -- Process Media, Ion Exchange Chromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Toyopearl SuperQ-650 media is a strong anion exchanger for biomolecule purification.  It can be used for high throughput capture, intermediate purification, and polishing process steps. If more powerful resolution is needed the smaller particle TSKgel SuperQ-5PW resin should be used. Selectivity remains the same.

Structure:

Product Attributes:

Literature:

Catalog -- Process Media, Ion Exchange Chromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

TSK-GEL DEAE-5PW media is a weak anion exchanger for biomolecule purification. Its small particle size makes it an excellent choice for polishing and high performance resolution steps. It has the same selectivity as the larger particle Toyopearl DEAE-650 resin.

Structure:

Product Attributes:

Literature:

Catalog -- Process Media, Ion Exchange Chromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

Comparative information for CIEX resins

TSKgel SP-5PW media is a strong cation exchanger for biomolecule purification. Its small particle size makes it an excellent choice for polishing and high performance resolution steps. It has the same selectivity as the larger particle Toyopearl SP-650 resin.

Structure:

Product Attributes:

Literature:

Catalog -- Process Media, Ion Exchange Chromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

TSKgel SuperQ-5PW media is a strong anion exchanger for biomolecule purification. Its small particle size makes it an excellent choice for polishing and high performance resolution steps. It has the same selectivity as the larger particle Toyopearl SuperQ-650 resin.

Structure:

Product Attributes:

Literature:

Catalog -- Process Media, Ion Exchange Chromatography

Technical Presentation -- High Resolution Anion Exchange Chromatography Purification of Oligonucleotides

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

*specifically designed for packed bed use 

Structure:

Product Attributes:

Literature:

Brochure -- Toyopearl GigaCap Ion Exchange Resins

Catalog -- Process Media, Ion Exchange Chromatography

Product Overview -- Toyopearl GigaCap CM-650M, for high capacity cation exchange chromatography of IgG

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

*specifically designed for packed bed use 

Structure:

Product Attributes:

Literature:

Application Note -- Toyopearl GigaCap S-650M, a very high capacity cation exchange resin

Brochure -- Toyopearl GigaCap Ion Exchange Resins

Catalog -- Process Media, Ion Exchange Chromatography

Journal Article -- Development of a High-Capacity MAb Capture Step Based on Cation-Exchange Chromatography   (BioProcess Int'l)

Product Overview -- Toyopearl GigaCap S-650M for High Capacity Cation Exchange Chromatography of IgG

Technical Presentation -- A High Capacity Strong Cation Exchange Resin for the Chromatographic Purification of Monoclonal Antibodies and Other Proteins

Technical Presentation -- Novel High Binding Capacity Ion Exchange Resins for Biochromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

*specifically designed for packed bed use 

Structure:

Product Attributes:

Literature:

Brochure -- Toyopearl GigaCap Ion Exchange Resins

Catalog -- Process Media, Ion Exchange Chromatography

Product Overview -- Toyopearl GigaCapQ-650M for high capacity Anion Exchange Chromatography of small and large proteins

Technical Presentation -- Novel High Binding Capacity Ion Exchange Resins for Biochromatography

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.