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Process Media - Resiner
Size Exclusion - Toyopearl HW - TSK-GEL PW
Toyopearl HW and TSK-GEL PW size exclusion resins are the foundation for all of Tosoh's ion exchange, hydrophobic interaction, and affinity process media.
After an initial polymerization reaction to create the designated particle size and pore size, the resin surface is chemically modified to create the desired final product.
Toyopearl HW Size Exclusion Beads
Toyopearl HW products are hydroxylated methacrylic polymer resins. Toyopearl HW beads are made commercially in many different pore sizes and particle diameters (30 micron and larger).
They vary from TSK-GEL PW beads by having a lower degree of crosslinking. Lower crosslinking makes available a larger number of resin sites for ligand immobilization when producing Toyopearl HW based ion exchange and hydrophobic interaction chromatography resins.
Therefore a functionalized Toyopearl HW resin will have a higher dynamic binding capacity than the corresponding TSK-GEL PW material.
Because similarly functionalized HW and PW resin types have the same backbone polymer chemistry, the selectivity for proteins and impurities remains the same.
Toyopearl HW based resin products can be used at high linear velocities and withstand operating pressures up to 3 bar.
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Resin Chemistry of Toyopearl and
TSK-GEL PW resins |
(Hydroxylated Acrylic)
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Comparison of Toyopearl HW resins and TSK-GEL PW resins offered by Tosoh Bioscience |
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TSK-GEL PW High Performance Size Exclusion Beads
TSK-GEL PW products are hydroxylated methacrylic polymer resins. TSK-GEL PW beads are made commercially in many different pore sizes and particle diameters (30 micron and smaller).
They vary from Toyopearl HW beads by having a higher degree of crosslinking (necessitated by the higher flow pressures needed when using smaller particles for chromatography). This decreases the number of resin sites available for ligand immobilization when producing TSK-GEL PW ion exchange and hydrophobic interaction chromatography resins.
A functionalized TSK-GEL PW resin will have a lower dynamic binding capacity than the corresponding Toyopearl HW material.
However, chromatographic efficiency, peak width, and resolution are improved because of their smaller particle sizes. In this way they are higher performing chromatography resins than the HW based materials.
Because similarly functionalized HW and PW resin types have the same backbone polymer chemistry, the elution selectivity for proteins and impurities remains the same.
TSK-GEL PW based resin products can be used at high linear velocities and withstand operating pressures up to 20 bar.
Toyopearl HW-40 resin is a hydroxylated methacrylic polymer for size exclusion chromatography. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da
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Structure:
Product Attributes:
| Pore size (mean): |
50Å |
| Exclusion limit: |
10,000 Da |
| Particle size (mean): |
75µm (C-grade)
45µm (F-grade)
30µm (S-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer |
20% ethanol |
| pH stability: |
2-13 |
| shelf life: |
10 years |
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Literature:
Catalog -- Process Media Catalog, Size Exclusion Chromatography
Operating Conditions and Specifications
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Comparative information for SEC resins |
Calibration curves for globular proteins on
Toyopearl HW resins
Typical resolution changes based on particle size
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Particle size |
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07450 |
Toyopearl HW-40EC |
500mL |
100-300µm |
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19807 |
Toyopearl HW-40C |
150mL |
50-100µm |
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07449 |
Toyopearl HW-40C |
500mL |
50-100µm |
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14683 |
Toyopearl HW-40C |
1L |
50-100µm |
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07969 |
Toyopearl HW-40C |
5L |
50-100µm |
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21484 |
Toyopearl HW-40C |
50L |
50-100µm |
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19808 |
Toyopearl HW-40F |
150mL |
30-60µm |
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07448 |
Toyopearl HW-40F |
500mL |
30-60µm |
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14682 |
Toyopearl HW-40F |
1L |
30-60µm |
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07968 |
Toyopearl HW-40F |
5L |
30-60µm |
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19809 |
Toyopearl HW-40S |
150mL |
20-40µm |
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07451 |
Toyopearl HW-40S |
250mL |
20-40µm |
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07447 |
Toyopearl HW-40S |
500mL |
20-40µm |
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14681 |
Toyopearl HW-40S |
1L |
20-40µm |
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07967 |
Toyopearl HW-40S |
5L |
20-40µm |
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Toyopearl HW-50 resin is a hydroxylated methacrylic polymer for size exclusion chromatography of proteins between 500-80,000 Da.
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Structure:
Product Attributes:
| Pore size (mean): |
125Å |
| Exclusion limit: |
80,000 Da |
| Particle size (mean): |
45µm (F-grade)
35µm (S-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer |
20% ethanol |
| pH stability: |
2-13 |
| shelf life: |
10 years |
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Literature:
Catalog -- Process Media Catalog, Size Exclusion Chromatography
Operating Conditions and Specifications
|
Comparative information for SEC resins |
Calibration curves for globular proteins on
Toyopearl HW resins
Typical resolution changes based on particle size
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Particle size |
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19810 |
Toyopearl HW-50F |
150mL |
30-60µm |
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07453 |
Toyopearl HW-50F |
500mL |
30-60µm |
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14685 |
Toyopearl HW-50F |
1L |
30-60µm |
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08060 |
Toyopearl HW-50F |
5L |
30-60µm |
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18368 |
Toyopearl HW-50F |
50L |
30-60µm |
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19811 |
Toyopearl HW-50S |
150mL |
20-40µm |
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07455 |
Toyopearl HW-50S |
250mL |
20-40µm |
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07452 |
Toyopearl HW-50S |
500mL |
20-40µm |
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14684 |
Toyopearl HW-50S |
1L |
20-40µm |
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08059 |
Toyopearl HW-50S |
5L |
20-40µm |
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Toyopearl HW-55 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 1,000-700,000 Da. It also is the base bead for all Toyopearl "-550" designated products.
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Structure:
Product Attributes:
| Pore size (mean): |
500Å |
| Exclusion limit: |
700,000 Da |
| Particle size (mean): |
45µm (F-grade)
30µm (S-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer |
20% ethanol |
| pH stability: |
2-13 |
| shelf life: |
10 years |
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Literature:
Catalog -- Process Media Catalog, Size Exclusion Chromatography
Operating Conditions and Specifications
|
Comparative information for SEC resins |
Calibration curves for globular proteins on
Toyopearl HW resins
Typical resolution changes based on particle size
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Particle size |
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19812 |
Toyopearl HW-55F |
150mL |
30-60µm |
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07457 |
Toyopearl HW-55F |
500mL |
30-60µm |
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14687 |
Toyopearl HW-55F |
1L |
30-60µm |
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08063 |
Toyopearl HW-55F |
5L |
30-60µm |
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21918 |
Toyopearl HW-55F |
50L |
30-60µm |
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19813 |
Toyopearl HW-55S |
150mL |
20-40µm |
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07459 |
Toyopearl HW-55S |
250mL |
20-40µm |
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07456 |
Toyopearl HW-55S |
500mL |
20-40µm |
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14686 |
Toyopearl HW-55S |
1L |
20-40µm |
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08062 |
Toyopearl HW-55S |
5L |
20-40µm |
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Toyopearl HW-65 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 40,000-5,000,000 Da. It also is the base bead for all Toyopearl "-650" designated products.
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Structure:
Product Attributes:
| Pore size (mean): |
1,000Å |
| Exclusion limit: |
5,000,000 Da |
| Particle size (mean): |
45µm (F-grade)
30µm (S-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer |
20% ethanol |
| pH stability: |
2-13 |
| shelf life: |
10 years |
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Literature:
Catalog -- Process Media Catalog, Size Exclusion Chromatography
Operating Conditions and Specifications
|
Comparative information for SEC resins |
Calibration curves for globular proteins on
Toyopearl HW resins
Typical resolution changes based on particle size
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Particle size |
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19814 |
Toyopearl HW-65F |
150mL |
30-60µm |
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07465 |
Toyopearl HW-65F |
500mL |
30-60µm |
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14689 |
Toyopearl HW-65F |
1L |
30-60µm |
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08069 |
Toyopearl HW-65F |
5L |
30-60µm |
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21852 |
Toyopearl HW-65F |
50L |
30-60µm |
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19815 |
Toyopearl HW-65S |
150mL |
20-40µm |
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07467 |
Toyopearl HW-65S |
250mL |
20-40µm |
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07464 |
Toyopearl HW-65S |
500mL |
20-40µm |
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14688 |
Toyopearl HW-65S |
1L |
20-40µm |
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08068 |
Toyopearl HW-65S |
5L |
20-40µm |
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18377 |
Toyopearl HW-65S |
50L |
20-40µm |
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21481 |
Toyopearl HW-65C |
150mL |
50-150µm |
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07466 |
Toyopearl HW-65C |
250mL |
50-150µm |
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14690 |
Toyopearl HW-65C |
1L |
50-150µm |
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08070 |
Toyopearl HW-65C |
5L |
50-150µm |
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21482 |
Toyopearl HW-65C |
50L |
50-150µm |
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Toyopearl HW-75 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 500,000-50,000,000 Da.
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Structure:
Product Attributes:
| Pore size (mean): |
>1,000Å |
| Exclusion limit: |
50,000,000 |
| Particle size (mean): |
45µm (F-grade)
30µm (S-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer |
20% ethanol |
| pH stability: |
2-13 |
| shelf life: |
10 years |
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Literature:
Catalog -- Process Media Catalog, Size Exclusion Chromatography
Operating Conditions and Specifications
|
Comparative information for SEC resins |
Calibration curves for globular proteins on
Toyopearl HW resins
Typical resolution changes based on particle size
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Particle size |
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19816 |
Toyopearl HW-75F |
150mL |
30-60µm |
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07469 |
Toyopearl HW-75F |
500mL |
30-60µm |
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14691 |
Toyopearl HW-75F |
1L |
30-60µm |
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08072 |
Toyopearl HW-75F |
5L |
30-60µm |
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07471 |
Toyopearl HW-75S |
250mL |
20-40µm |
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07468 |
Toyopearl HW-75S |
500mL |
20-40µm |
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08071 |
Toyopearl HW-75S |
5L |
20-40µm |
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For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.
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MD Scientific ApS • Denmark • tlf. 7027 8565 • fax 7027 8566 • info@md-scientific.dk |
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