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Process Media Products
Toyopearl HW Size Exclusion resin
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Click on each resin to get more information |
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The chart above shows the molecular weight ranges of each pore size Toyopearl HW Size Exclusion resin. Click on the individual calibration bar for more resin information.
The SEC resins are the base beads for our surface modified Ion Exchange, Hydrophobic Interaction, Affinity and Custom chromatography products. These use the following naming convention:
"Toyopearl" + "ligand" + "-SEC resin number" + "0".
So "Toyopearl" + "Butyl" + "-65(from HW-65)" + "0" = "Toyopearl Butyl-650"
The availability of multiple pore sizes enables Tosoh Bioscience to put the same ligand on different pore size base beads (some not offered as SEC resins) for different customer applications.
For example:
- Toyopearl Butyl-650 (HW-65 base bead) - for larger proteins
- Toyopearl Butyl-600 (HW-60 base bead) - for IgG size targets
- Toyopearl SuperButyl-550 (HW-55 base bead) - for smaller proteins
Each product is designed for a different molecular weight range and customer feedstock profile. |
Toyopearl HW-40 resin is a hydroxylated methacrylic polymer for size exclusion chromatography. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da
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Structure:
Product Attributes:
| Pore size (mean): |
50Å |
| Exclusion limit: |
10,000 Da |
| Particle size (mean): |
75µm (C-grade)
45µm (F-grade)
30µm (S-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer |
20% ethanol |
| pH stability: |
2-13 |
| shelf life: |
10 years |
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Literature:
Catalog -- Process Media Catalog, Size Exclusion Chromatography
Operating Conditions and Specifications
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Comparative information for SEC resins |
Calibration curves for globular proteins on
Toyopearl HW resins
Typical resolution changes based on particle size
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Particle size |
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07450 |
Toyopearl HW-40EC |
500mL |
100-300µm |
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19807 |
Toyopearl HW-40C |
150mL |
50-100µm |
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07449 |
Toyopearl HW-40C |
500mL |
50-100µm |
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14683 |
Toyopearl HW-40C |
1L |
50-100µm |
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07969 |
Toyopearl HW-40C |
5L |
50-100µm |
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21484 |
Toyopearl HW-40C |
50L |
50-100µm |
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19808 |
Toyopearl HW-40F |
150mL |
30-60µm |
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07448 |
Toyopearl HW-40F |
500mL |
30-60µm |
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14682 |
Toyopearl HW-40F |
1L |
30-60µm |
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07968 |
Toyopearl HW-40F |
5L |
30-60µm |
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19809 |
Toyopearl HW-40S |
150mL |
20-40µm |
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07451 |
Toyopearl HW-40S |
250mL |
20-40µm |
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07447 |
Toyopearl HW-40S |
500mL |
20-40µm |
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14681 |
Toyopearl HW-40S |
1L |
20-40µm |
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07967 |
Toyopearl HW-40S |
5L |
20-40µm |
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Toyopearl HW-50 resin is a hydroxylated methacrylic polymer for size exclusion chromatography of proteins between 500-80,000 Da.
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Structure:
Product Attributes:
| Pore size (mean): |
125Å |
| Exclusion limit: |
80,000 Da |
| Particle size (mean): |
45µm (F-grade)
35µm (S-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer |
20% ethanol |
| pH stability: |
2-13 |
| shelf life: |
10 years |
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Literature:
Catalog -- Process Media Catalog, Size Exclusion Chromatography
Operating Conditions and Specifications
|
Comparative information for SEC resins |
Calibration curves for globular proteins on
Toyopearl HW resins
Typical resolution changes based on particle size
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Particle size |
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19810 |
Toyopearl HW-50F |
150mL |
30-60µm |
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07453 |
Toyopearl HW-50F |
500mL |
30-60µm |
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14685 |
Toyopearl HW-50F |
1L |
30-60µm |
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08060 |
Toyopearl HW-50F |
5L |
30-60µm |
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18368 |
Toyopearl HW-50F |
50L |
30-60µm |
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19811 |
Toyopearl HW-50S |
150mL |
20-40µm |
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07455 |
Toyopearl HW-50S |
250mL |
20-40µm |
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07452 |
Toyopearl HW-50S |
500mL |
20-40µm |
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14684 |
Toyopearl HW-50S |
1L |
20-40µm |
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08059 |
Toyopearl HW-50S |
5L |
20-40µm |
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Toyopearl HW-55 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 1,000-700,000 Da. It also is the base bead for all Toyopearl "-550" designated products.
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Structure:
Product Attributes:
| Pore size (mean): |
500Å |
| Exclusion limit: |
700,000 Da |
| Particle size (mean): |
45µm (F-grade)
30µm (S-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer |
20% ethanol |
| pH stability: |
2-13 |
| shelf life: |
10 years |
|
Literature:
Catalog -- Process Media Catalog, Size Exclusion Chromatography
Operating Conditions and Specifications
|
Comparative information for SEC resins |
Calibration curves for globular proteins on
Toyopearl HW resins
Typical resolution changes based on particle size
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Particle size |
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19812 |
Toyopearl HW-55F |
150mL |
30-60µm |
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07457 |
Toyopearl HW-55F |
500mL |
30-60µm |
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14687 |
Toyopearl HW-55F |
1L |
30-60µm |
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08063 |
Toyopearl HW-55F |
5L |
30-60µm |
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21918 |
Toyopearl HW-55F |
50L |
30-60µm |
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19813 |
Toyopearl HW-55S |
150mL |
20-40µm |
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07459 |
Toyopearl HW-55S |
250mL |
20-40µm |
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07456 |
Toyopearl HW-55S |
500mL |
20-40µm |
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14686 |
Toyopearl HW-55S |
1L |
20-40µm |
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08062 |
Toyopearl HW-55S |
5L |
20-40µm |
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Toyopearl HW-65 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 40,000-5,000,000 Da. It also is the base bead for all Toyopearl "-650" designated products.
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Structure:
Product Attributes:
| Pore size (mean): |
1,000Å |
| Exclusion limit: |
5,000,000 Da |
| Particle size (mean): |
45µm (F-grade)
30µm (S-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer |
20% ethanol |
| pH stability: |
2-13 |
| shelf life: |
10 years |
|
Literature:
Catalog -- Process Media Catalog, Size Exclusion Chromatography
Operating Conditions and Specifications
|
Comparative information for SEC resins |
Calibration curves for globular proteins on
Toyopearl HW resins
Typical resolution changes based on particle size
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Particle size |
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19814 |
Toyopearl HW-65F |
150mL |
30-60µm |
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07465 |
Toyopearl HW-65F |
500mL |
30-60µm |
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14689 |
Toyopearl HW-65F |
1L |
30-60µm |
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08069 |
Toyopearl HW-65F |
5L |
30-60µm |
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21852 |
Toyopearl HW-65F |
50L |
30-60µm |
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19815 |
Toyopearl HW-65S |
150mL |
20-40µm |
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07467 |
Toyopearl HW-65S |
250mL |
20-40µm |
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07464 |
Toyopearl HW-65S |
500mL |
20-40µm |
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14688 |
Toyopearl HW-65S |
1L |
20-40µm |
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08068 |
Toyopearl HW-65S |
5L |
20-40µm |
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18377 |
Toyopearl HW-65S |
50L |
20-40µm |
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21481 |
Toyopearl HW-65C |
150mL |
50-150µm |
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07466 |
Toyopearl HW-65C |
250mL |
50-150µm |
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14690 |
Toyopearl HW-65C |
1L |
50-150µm |
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08070 |
Toyopearl HW-65C |
5L |
50-150µm |
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21482 |
Toyopearl HW-65C |
50L |
50-150µm |
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Toyopearl HW-75 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 500,000-50,000,000 Da.
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Structure:
Product Attributes:
| Pore size (mean): |
>1,000Å |
| Exclusion limit: |
50,000,000 |
| Particle size (mean): |
45µm (F-grade)
30µm (S-grade) |
| Pressure rating: |
3 bar |
| Shipping buffer |
20% ethanol |
| pH stability: |
2-13 |
| shelf life: |
10 years |
|
Literature:
Catalog -- Process Media Catalog, Size Exclusion Chromatography
Operating Conditions and Specifications
|
Comparative information for SEC resins |
Calibration curves for globular proteins on
Toyopearl HW resins
Typical resolution changes based on particle size
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Resin volume |
Particle size |
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19816 |
Toyopearl HW-75F |
150mL |
30-60µm |
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07469 |
Toyopearl HW-75F |
500mL |
30-60µm |
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14691 |
Toyopearl HW-75F |
1L |
30-60µm |
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08072 |
Toyopearl HW-75F |
5L |
30-60µm |
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07471 |
Toyopearl HW-75S |
250mL |
20-40µm |
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07468 |
Toyopearl HW-75S |
500mL |
20-40µm |
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08071 |
Toyopearl HW-75S |
5L |
20-40µm |
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For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.
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