TSK-GEL® kolonner

TSKgel Boronate-5PW

Coupling of m-aminophenyl boronate to the TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers.  Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris. 

Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.

Structure:

Product Attributes:

Literature:

Catalog -- Chromatography Catalog, AFC Columns

Instruction Manual -- Guardgel Kits for TSK-GEL HPLC Columns

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

TSKgel Chelate-5PW utilizes the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation.  Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.

Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.

Structure:

Product Attributes:

Literature:

Catalog -- Chromatography Catalog, AFC Columns

Instruction Manual -- Guardgel Kits for TSK-GEL HPLC Columns

Operating Conditions and Specifications

Operating Conditions and Specifications (BioAssist)

Separation Report -- Separation of Monoclonal Antibodies Using TSK-GEL HPLC Columns

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

TSKgel Heparin-5PW

The heparin ligand in the TSKgel Heparin-5PW affinity column can interact with sample molecules by cation exchange as well as affinity chromatography. Cation exchange interaction takes place through O-sulfate and N-sulfate groups. The elution strength is similar to TSKgel SP-5PW.

In vivo, heparin prevents blood clotting as it reacts with clotting agents. This tendency is taken advantage of by using heparin to separate blood clotting factors such as antithrombin III, Factor VII, Factor IX,etc., and anti-heparin proteins such as glycoproteins, endoglycosidase, hyaluronidase, lipases, growth factors, RNA polymerases, and other nucleic acid-binding proteins.

Structure:

Product Attributes:

Literature:

Catalog -- Chromatography Catalog, AFC Columns

Operating Conditions and Specifications

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

TSKgel Tresyl-5PW

Unlike other TSK-GEL affinity columns, TSKgel Tresyl-5PW, which is derivatized with the 2,2,2-trifluroethanesulfonyl ligand, requires activation with a user-selected ligand containing amino, thiol, phenol, or imidazole groups.  The resulting structure is literally a "custom" affinity ligand with excellent pH stability and minimal ligand loss due to leaching. TSKgel Tresyl-5PW readily reacts with amino or thiol groups to form stable covalent alkylamines or thioethers.

 Applications include: glycoproteins, antigens, etc.

Structure:

Product Attributes:

Literature:

Catalog -- Chromatography Catalog, AFC Columns

Operating Conditions and Specifications 

Separation Report -- Separation of Monoclonal Antibodies Using TSK-GEL HPLC Columns

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.