Tosoh TSK-GEL - Hydrophobic interaction

TSK-GEL® kolonner Hydrophobic Interaction Chromatography

		Startside for Tosoh kolonner

TSK-GEL HIC packings consist of polymethacrylate base material and a choice of three ligands (butyl, ether and phenyl) with varied hydrophobicities from low to high, respectively.
The polymethacrylate base resin for the ether and phenyl columns is a porous material with a 1000Å pore size and 1,000,000 Da exclusion limit, offering high capacity and high sample loads. The TSKgel Butyl-NPR features a non-porous support in which only the surface of the bead is available for adsorption. Shorter run times and excellent recovery result, making the butyl columns an optimal choice for high throughput or QC analysis. See the figure below for a comparison between the three column types.

Features

Benefits

TSKgel Phenyl-5PW

Most

hydrophobic

Requires modest salt concentration to retain proteins. Most popular column applicable for the widest range of hydrophobicities

TSKgel Ether-5PW

Less

hydrophobic

Excellent choice for hydrophobic proteins such as membrane proteins or monoclonal antibodies.

TSKgel Butyl-NPR

Least
hydrophobic

Excellent choice for high speed applications Usually high recovery due to absence of pores.

TSKgel Phenyl-5PW

The most hydrophobic among the three TSK-GEL HIC columns, TSKgel Phenyl-5PW is an excellent choice to screen for the selectivity, retention and recovery of most biomolecules. Featuring a 1000Å pore size in a polymethacrylate base material, the Phenyl-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients.

Stainless steel is the standard column format for TSKgel Phenyl-5PW. Glass and PEEK column housings are available for samples that may adsorb to stainless steel. The alternative column formats are also suitable for general applications.

Structure:

Phenyl-5PW Chemical Structure

Product Attributes:

Pore size (mean):	1,000Å
Particle size (mean):	10, 13 and 20µm
pH stability:	2.0 - 12.0
Functional group:	Phenyl

Literature:

Catalog -- Chromatography Catalog, HIC Columns

Instruction Manual -- Guardgel Kits for TSK-GEL HPLC Columns

Operating Conditions and Specifications

Separation Report -- Separation of Monoclonal Antibodies Using TSK-GEL HPLC Columns

Separation Report -- Application of TSK-GEL Semi-Micro Columns for High-Sensitivity Analyses

Applications

Retain large RNAs on TSKgel Phenyl-5PW

Scale up to preparative separations

Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.

P/N	Description	Particle size	Housing material	ID (mm)	Length (cm)
13063	TSKgel Phenyl-5PW	10µm	Glass	5	5
08804	TSKgel Phenyl-5PW	10µm	Glass	8	7.5
18759	TSKgel Phenyl-5PW	10µm	Stainless Steel	2	7.5
42155	Guard Cartridge for P/N 18759		Stainless Steel	2	1
19308	Guard Cartridge Holder for P/N 42155		Stainless Steel	2	1
07573	TSKgel Phenyl-5PW	10µm	Stainless Steel	7.5	7.5
07652	Guardgel Kit for P/N 07573		Stainless Steel
07656	TSKgel Phenyl-5PW	13µm	Stainless Steel	21.5	15
16095	Guardgel Kit for P/N 07656		Stainless Steel
07938	TSKgel Phenyl-5PW	20µm	Stainless Steel	55	20
07936	Guard Column for P/N 07938		Stainless Steel	45	5
20023	TSKgel BioAssist Phenyl	10µm	PEEK	7.8	5

TSKgel Ether-5PW

Of the three TSK-GEL HIC columns, TSKgel Ether-5PW has intermediate hydrophobity. It is an excellent choice for separating hydrophobic molecules such as membrane proteins or monoclonal antibodies such as IgG or IgM. Featuring a 1000Å pore size in a polymethacrylate base material, the Ether-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients.