|
|
Tosoh Bioscience offers a broad line of high efficiency columns for analysis and isolation of biomolecules by ion exchange chromatography. For Anion as well as Cation Exchange Chromatography, the product line contains methacrylate-based and silica-based columns for the analysis of proteins, peptides, DNA- and RNA- derived oligonucleotides and other nucleic acid fragments.
Polystyrene-based ion exchange columns are available for analyzing low MW sugars, amino acids, nucleic acid bases, and small drug candidates.
Hydrophilic polymer-based, exchange columns packed with non-porous resin particles are available for the separation of proteins, protein aggregates, charge isomers of monoclonal antibodies, PEGylated proteins, DNA fragments, nucleic acids, oligo DNA, and siRNA.
Specialty columns are available for organic acids by ion-exclusion (OApak-A and SCX) and mono- and disaccharides (Sugar AXI and Sugar AXG).
Common Characteristics of TSK-GEL Anion and Cation Exchangers |
The methacrylate base resin, G5000PW (5PW), is a spherical 10μm particle with approximately 1000Å pores. TSK-GEL 5PW is derivatized with diethylaminoethyl (DEAE), sulfopropyl (SP) or carboxymethyl (CM) functionalities to provide a weak anion, a strong cation, and a weak cation exchanger, respectively. The polyamine chemistry employed in TSKgel SuperQ-5PW results in a high capacity strong anion exchanger with a smaller effective pore size than TSKgel DEAE-5PW.
Particles in TSKgel BioAssist Q columns contain very large pores (~4000Å) that are functionalized with polyamine groups to form a network structure. The capacity of BioAssist Q columns is high over a wide molecular weight range. TSK-GEL BioAssist S columns are packed with particles possessing 1300Å pores functionalized with sulfopropyl groups.
Methacrylate is also the backbone of non-porous resin (NPR) columns such as DEAE-NPR, DNA-NPR and SP-NPR, which are packed with 2.5μm particles. High column efficiency coupled with low sample capacity restricts the application of these columns to fast analysis and micro-scale preparative isolation. Due to the absence of large pores, protein recovery is generally very high on NPR columns.
TSK-GEL STAT Series non-porous resin columns contain large particles (5, 7 and 10μm). This relatively large particle size, combined with an innovative bonding chemistry, results in columns that enable fast equilibration and analysis of complex biomolecular samples.
|
| Anion Exchange Columns (type, matrix) |
Cation Exchange Columns (type, matrix) |
-
Strong anion, methacrylic
-
Strong anion, silica
-
Strong anion, polymer
-
Strong anion, polystyrene
-
Weak anion, methacrylic
-
Weak anion, silica
-
Strong anion, polymer
|
-
Strong cation, methacrylic
-
Strong cation, silica
-
Strong cation, polymer
-
Strong cation, polystyrene
-
Weak cation, methacrylic
- Weak cation, silica
-
Weak cation, polymer
|
|
Anion Exchange Columns
Anion exchange chromatography is practiced with either a strong or a weak anion exchange column, containing a quarternary ammonium ion, or with a weak anion exchanger, having either a tertiary or secondairy amine functional group, such as DEAE (diethylaminoethyl). A counter ion, often Cl- maintains electroneutrality.
In ion exchange chromatography the pH of the mobile phase buffer must be between the pI or pKa of the charged molecule and the pKa of the charged groups on the solid support. For instance, in anion exchange chromatography a molecule with a pI of 6.8 is run in a mobile phase buffer at pH 8.0 with the solid support pKa at 10.3.
Speciality Column
- Oligonucleotide fragments, PCR reagents (DNA-NPR)
|
Highlights:
- Pore structure and bonding chemistry of BioAssist Q columns provides high capacity for small to very large molecular weight proteins and nucleic acids
- TSKgel SuperQ-5PW columns have higher capacity than TSKgel DEAE-5PW due to novel bonding chemistry; effective pore size is smaller for SuperQ-5PW
- Binding capacity for small to medium size proteins on TSKgel DEAE-3SW is roughly double that of the 5PW packings due to the smaller pore size and larger surface area.
- TSKgel DEAE-5PW and DEAE-2SW columns are available in 2mm ID format
- Specialty columns for analysis of mono- and disaccharides, sugar alcohols, and organic acids.
- TSKgel DNA-NPR columns for PCR fragment analysis
- BioAssist columns are packed in PEEK hardware, while other columns are available in glass and stainless steel.
- Very efficient chromatography can be achieved with the TSK-GEL STAT columns due to the novel bonding chemistry and the absence of micro-pores.
|
Strong Anion Exchange |
Weak Anion Exchange |
|
TSKgel BioAssist Q
TSKgel DEAE-2SW
TSKgel DEAE-2SW columns are packed with 5µm porous spherical silica beads which are surface modified with a weak anion exchanger. They are used for the separation and analysis of nucleotides, nucleosides, peptides, small proteins and other biologically active molecules. A 2mm ID column is available for applications that are sample limited.
|
Product Attributes:
| Matrix: |
hydrophilic silica |
| Particle size (mean): |
5µm |
| Pore size (mean): |
125Å |
| Functional group: |
CH2CH2N+(C2H5)3 |
| Counter ion: |
H2PO4- |
| pH range: |
2.0 -7.5 |
Capacity
(mg BSA/mL): |
ND |
| Small ion capacity: |
>0.3 meq/mL |
| pKa: |
11.2 |
|
Literature:
Catalog -- Chromatography Catalog, IEC Columns, Overview
Catalog -- Chromatography Catalog, IEC Columns, Anion
Instruction Manual -- Guardgel Kits for TSK-GEL HPLC Columns
Operating Conditions and Specifications
Application Overview -- Purification of Nucleic Acids
Separation Report -- Application of TSK-GEL Semi-Micro Columns for High-Sensitivity Analyses
|
Applications |
Separation of nucleotides with TSKgel DEAE-2SW
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
18761 |
TSKgel DEAE-2SW |
5µm |
Stainless Steel |
2 |
25 |
07168 |
TSKgel DEAE-2SW |
5µm |
Stainless Steel |
4.6 |
25 |
|
Guard Cartridge 2mmID for P/N 18761, 3pk |
|
Stainless Steel |
2 |
1 |
19308 |
Guard Cartridge Holder for P/N 42154 |
|
Stainless Steel |
2 |
1 |
07648 |
Guardgel Kit for P/N 07168 |
|
|
|
|
|
|
TSKgel DEAE-3SW
TSKgel DEAE-3SW columns are packed with 10µm porous spherical silica beads which are chemically modified with a weak anion exchanger. They are used for the separation and analysis of proteins, peptides, nucleotides, nucleosides and other biologically active molecules.
|
Product Attributes:
| Matrix: |
hydrophilic silica |
| Particle size (mean): |
10µm |
| Pore size (mean): |
250Å |
| Functional group: |
CH2CH2N+(C2H5)3 |
| Counter ion: |
Cl- |
| pH range: |
2.0 - 7.5 |
Capacity
(mg BSA/mL): |
ND |
| Small ion capacity: |
>0.3 meq/mL |
| pKa: |
11.2 |
|
Literature:
Catalog -- Chromatography Catalog, IEC Columns, Overview
Catalog -- Chromatography Catalog, IEC Columns, Anion
Instruction Manual -- Guardgel Kits for TSK-GEL HPLC Columns
Operating Conditions and Specifications
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
07163 |
TSKgel DEAE-3SW |
10µm |
Stainless Steel |
7.5 |
7.5 |
07648 |
Guardgel Kit for P/N 07163 |
|
Stainless Steel |
|
|
|
|
TSKgel DEAE-5PW
TSKgel DEAE-5PW columns are packed with porous hydrophilic polymer beads which are surface modified with a weak anion exchanger. They are used for the separation and analysis of proteins, nucleotides, nucleosides and other biologically active molecules. Columns are available in internal diameters varying from 2mm to 55mm ID. Column housings are either glass or stainless steel.
They have the same backbone chemistry and selectivity as the bulk process scale resins Toyopearl DEAE-650 and TSK-GEL DEAE-5PW.
|
Product Attributes:
| Matrix: |
hydroxylated methacrylate polymer |
| Particle size (mean): |
10, 13, 20µm |
| Pore size (mean): |
1000Å |
| Functional group: |
CH2CH2N+(C2H5)3 |
| Counter ion: |
Cl- |
| pH range: |
2.0 -12.0 |
Capacity
(mg BSA/mL): |
30 |
| Small ion capacity: |
0.1 meq/mL |
| pKa: |
11.5 |
|
Literature:
Catalog -- Chromatography Catalog, IEC Columns, Overview
Catalog -- Chromatography Catalog, IEC Columns, Anion
Instruction Manual -- Guardgel Kits for TSK-GEL HPLC Columns
Operating Conditions and Specifications
Application Overview -- Purification of Nucleic Acids
Separation Report -- Separation of Monoclonal Antibodies Using TSK-GEL HPLC Columns
Separation Report -- Application of TSK-GEL Semi-Micro Columns for High-Sensitivity Analyses
|
Applications |
Large pore TSKgel DEAE-5PW resolves high MW RNA
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
13061 |
TSKgel DEAE-5PW |
|
Glass |
5 |
5 |
08802 |
TSKgel DEAE-5PW |
10µm |
Glass |
8 |
7.5 |
|
Glass Guardgel Kit for P/N 13061, 08802 |
|
Glass |
|
|
|
TSKgel DEAE-5PW |
|
Glass |
20 |
15 |
14466 |
Glass Guard Column for P/N 14016 |
|
Glass |
|
|
|
TSKgel DEAE-5PW |
|
Stainless Steel |
2 |
7.5 |
|
Guard Cartridge for P/N 18757 |
|
Stainless Steel |
2 |
1 |
|
Cartridge Holder for P/N 42152 |
|
Stainless Steel |
2 |
1 |
|
TSKgel DEAE-5PW |
|
Stainless Steel |
7.5 |
7.5 |
|
Guardgel Kit for P/N 07164 |
|
Stainless Steel |
|
|
|
TSKgel DEAE-5PW |
13µm |
Stainless Steel |
21.5 |
15 |
|
Guardgel Kit for P/N 07574 |
|
Stainless Steel |
|
|
|
TSKgel DEAE-5PW |
20µm |
Stainless Steel |
55 |
20 |
07928 |
Guard Column for P/N 07930 |
|
Stainless Steel |
45 |
5 |
|
|
TSKgel DEAE-NPR
TSKgel DEAE-NPR columns are packed with spherical 2.5 micron, non-porous, high efficiency, hydrophilic polymer beads which are surface modified with a weak anion exchanger. They are used for the high speed separation and analysis of proteins and poly- and oligonucleotides.
They are particularly useful for high resolution separation of DNA digests or fragments.
|
Product Attributes:
| Matrix: |
hydroxylated methacrylic polymer |
| Particle size (mean): |
2.5µm |
| Pore size (mean): |
non-porous |
| Functional group: |
CH2CH2N+(C2H5)3 |
| Counter ion: |
Cl- |
| pH range: |
2.0 - 12 |
Capacity
(mg BSA/mL): |
5 |
| Small ion capacity: |
>0.1 meq/mL |
| pKa: |
11.2 |
|
Literature:
Catalog -- Chromatography Catalog, IEC Columns, Overview
Catalog -- Chromatography Catalog, IEC Columns, Anion
Operating Conditions and Specifications
Application Overview -- Purification of Nucleic Acids
Separation Report -- Separation of Monoclonal Antibodies Using TSK-GEL HPLC Columns
|
Applications |
Higher resolution and faster analysis with
TSK-GEL NPR columns
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
13075 |
TSKgel DEAE-NPR |
2.5 |
Stainless Steel |
4.6 |
3.5 |
17088 |
Guard Column for P/N13075 |
5 |
Stainless Steel |
4.6 |
0.5 |
|
|
TSKgel DNA-NPR
TSKgel DNA-NPR columns are packed with 2.5 micron, non-porous, high efficiency, hydrophilic polymer beads which are surface modified with a weak anion exchanger. Because NPR columns contain non-porous particles, binding capacity is low compared to porous columns with the same ligand functionality.
Column dimensions are optimized for the high efficiency separation of DNA fragments, PCR products, or plasmids. A small guard column is available to protect the analytical column.
|
Product Attributes:
| Matrix: |
hydroxylated methacrylic polymer |
| Particle size (mean): |
2.5µm |
| Pore size (mean): |
non-porous |
| Functional group: |
proprietary |
| Counter ion: |
ClO4- |
| pH range: |
2-12 |
Capacity
(mg BSA/mL): |
5 |
| Small ion capacity: |
>0.1 meq/mL |
| pKa: |
11.2 |
|
Literature:
Application Note -- Purification of a 13-mer DNA Phosphorothioated Crude Deprotected Oligonucleotide by Strong Anion Exchange Chromatography Using TSKgel DNA- NPR
Catalog -- Chromatography Catalog, IEC Columns, Overview
Catalog -- Chromatography Catalog, IEC Columns, Anion
Operating Conditions and Specifications
Product Overview -- TSK-GEL DNA-NPR Anion Exchange Columns
Application Overview -- Purification of Nucleic Acids
|
Applications |
Plasmid analysis with TSKgel DNA-NPR column
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
18249 |
TSKgel DNA-NPR |
2.5µm |
Stainless Steel |
4.6 |
7.5 |
18253 |
Guard Column for P/N 18249 |
5 |
Stainless Steel |
4.6 |
0.5 |
|
|
TSKgel DNA-STAT
TSK-GEL DNA-STAT columns are packed with 5µm hydrophilic non-porous resin particles of which the surface consists of an open access network of multi-layered anion exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity, low operating pressure and rapid analysis.
Applications for the TSK-GEL DNA-STAT columns include the separation of: DNA fragments, nucleic acids and nucleotides.
|
Product Attributes:
| Matrix: |
hydrophilic polymer |
| Particle size (mean): |
5µm |
| Pore size (mean): |
non-porous |
| Functional group: |
quarternary ammonium |
| Counter ion: |
Cl |
| pH range: |
3.0 - 10.0 |
Static binding capacity(mg BSA/mg dry gel): |
ca. 35 (5µm) |
| Small ion capacity: |
270ueg/g dry gel |
| pKa: |
10.5 |
|
Literature:
Technical Presentation -- High Speed and High Resolution Anion Exchange Chromatography for Biological Samples on Non-Porous Packings
Operating Conditions and Specifications
Product Overview -- TSK-GEL DNA-STAT Anion Exchange Column
|
Applications |
Large DNA fragments containing more than
10kb were successfully separated on a
TSKgel DNA-STAT column.
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
21962 |
TSKgel DNA-STAT |
5µm |
Stainless Steel |
4.6 |
10 |
|
|
TSKgel QAE-2SW
TSKgel Q-STAT
TSK-GEL Q-STAT columns are packed with 7 and 10µm hydrophilic non-porous resin particles of which the surface consists of an open access network of multi-layered anion exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity and a low operating pressure, making these columns suitable for all HPLC and FPLC systems.
Applications for the TSK-GEL Q-STAT columns include the separation of: proteins, peptides, low molecular weight nucleic acids, aggregates and charge isomers of monoclonal antibodies, PEGylated proteins, oligo DNA, and siRNA.
|
Product Attributes:
| Matrix: |
hydrophilic polymer |
| Particle size (mean): |
7µm and 10µm |
| Pore size (mean): |
non-porous |
| Functional group: |
quaternary ammonium |
| Counter ion: |
Cl |
| pH range: |
3.0 - 10.0 |
Static binding
capacity (mg BSA/
mg dry gel): |
ca. 25 (7µm)
ca. 20 (10µm) |
| Small ion capacity: |
270ueq/g dry gel |
| pKa: |
10.5 |
|
Literature:
Technical Presentation -- High Speed and High Resolution Anion Exchange Chromatography for Biological Samples on Non-Porous Packings
Operating Conditions and Specifications
Product Overview -- TSK-GEL Q-STAT Anion Exchange Column
|
Applications |
The protein mixture was completely separated within 1 minute and with higher resolution on the
TSKgel Q-STAT column in comparison to the monolithic WAX column.
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
21960 |
TSKgel Q-STAT |
10µm |
Stainless Steel |
3 |
3.5 |
21961 |
TSKgel Q-STAT |
7µm |
Stainless Steel |
4.6 |
10 |
|
|
TSKgel SAX
TSKgel Sugar AXG
TSK-GEL Sugar AXG columns are packed with 10µm porous spherical polymer beads which are surface modified with a strong anion exchanger. They are used for the gradient separation and analysis of monosaccharides, disaccharides, and sugar alcohols.
For isocratic separation of carbohydrates where lower and constant back pressures may be generated, please see the TSKgel Sugar AXI which is packed with 8µm particles.
|
Product Attributes:
| Matrix: |
PS-DVB polymer |
| Particle size (mean): |
10µm |
| Pore size (mean): |
60Å |
| Functional group: |
trimethylamino |
| Counter ion: |
HBO3- |
| pH range: |
1 - 14 |
| Capacity : |
NA |
| Small ion capacity: |
>1.2 meq/mL |
| pKa: |
12.5 |
|
Literature:
Catalog -- Chromatography Catalog, IEC Columns, Overview
Catalog -- Chromatography Catalog, IEC Columns, Anion
Operating Conditions and Specifications
|
Applications |
Separation of saccharide mixture on
TSKgel Sugar AXG
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
08640 |
TSKgel Sugar AXG |
10µm |
Stainless Steel |
4.6 |
15 |
|
|
TSKgel Sugar AXI
TSK-GEL Sugar AXI columns are packed with 8µm porous spherical polymer beads which are modified with a strong anion exchanger. They are used for the isocratic separation and analysis of monosaccharides, disaccharides, and sugar alcohols.
For gradient separation of carbohydrates where higher back pressures may be generated, please see the TSKgel Sugar AXG which is packed with 10µm particles.
|
Product Attributes:
| Matrix: |
PS-DVB polymer |
| Particle size (mean): |
8µm |
| Pore size (mean): |
60Å |
| Functional group: |
trimethylamino |
| Counter ion: |
HBO3- |
| pH range: |
1.0 - 14.0 |
| Capacity : |
ND |
| Small ion capacity: |
>1.2meq/mL |
| pKa: |
12.5 |
|
Literature:
Catalog -- Chromatography Catalog, IEC Columns, Overview
Catalog -- Chromatography Catalog, IEC Columns, Anion
Operating Conditions and Specifications
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
08639 |
TSKgel Sugar AXI |
8µm |
Stainless Steel |
4.6 |
15 |
|
|
TSKgel SuperQ-5PW
TSK-GEL SuperQ-5PW columns are packed with porous hydrophilic polymer beads which are modified with a strong anion exchanger. The resulting product exhibits very high protein capacities. TSKgel SuperQ-5PW is used for the separation and analysis of proteins, oligonucleotides and other biologically active molecules.
TSK-GEL SuperQ-5PW columns have the same backbone chemistry and selectivity as the bulk process scale resins TSK-GEL SuperQ-5PW and Toyopearl SuperQ-650.
|
Product Attributes:
| Matrix: |
polymethacrylate |
| Particle size (mean): |
10µm and 13µm |
| Pore size (mean): |
1000Å |
| Functional group: |
trimethylamino |
| Counter ion: |
Cl- |
| pH range: |
2.0 - 12.0 |
| Capacity: |
100mg BSA/mL |
| Small ion capacity: |
>0.13meq/mL |
| pKa: |
12.2 |
|
Literature:
Catalog -- Chromatography Catalog, IEC Columns, Overview
Catalog -- Chromatography Catalog, IEC Columns, Anion
Instruction Manual -- Guardgel Kits for TSK-GEL HPLC Columns
Operating Conditions and Specifications
|
Applications |
Analysis of synthetic oligonucleotide on
TSKgel SuperQ-5PW
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
|
TSKgel SuperQ-5PW |
|
Glass |
8 |
|
18257 |
TSKgel SuperQ-5PW |
10µm |
Stainless Steel |
7.5 |
7.5 |
18388 |
Guardgel Kit for P/N 18257 |
|
Stainless Steel |
|
|
18387 |
TSKgel SuperQ-5PW |
13µm |
Stainless Steel |
21.5 |
15 |
|
|
Cation Exchange Columns
Cation exchange chromatography is practiced with either a strong or a weak cation exchange column, containing a sulfonium ion, or with a weak cation exchanger, having usually a carboxymethyl (CM) functional group. A counter ion, often Na+ maintains electroneutrality.
In ion exchange chromatography the pH of the mobile phase buffer must be between the pI or pKa of the charged molecule and the pKa of the charged groups on the solid support. For example, a molecule with a pI of 8.2 is run in a mobile phase buffer at pH 6.0 with the solid support pKa at 1.2 in cation exchange chromatography.
Specialty columns:
|
Highlights
- Pore structure and bonding chemistry of TSKgel BioAssist S provides high capacity for medium to large molecular weight proteins.
- BioAssist columns are packed in 4.6mmID or 10mmID PEEK hardware. Other columns are available in glass and stainless steel for anlytical, semi-preparative and preparative applications.
- Binding capacity for small to medium size proteins on TSKgel DEAE-3SW is approximately double that of the 5PW packings due to the smaller pore size and larger surface area.
- TSKgel sp-5PW column available in 2mmID format.
- Very efficient chromatography can be achieved with the TSK-GEL STAT columns due to the novel bonding chemistry and the absence of micro-pores.
|
Strong Cation Exchange |
Weak Cation Exchange |
Polymethacrylate:
Silica: TSKgel SP-2SW
Polystyrene: TSKgel SCX
Polymer: TSKgel SP-STAT |
Polymethacrylate:
Silica:
Polymer: TSKgel CM-STAT |
|
TSKgel BioAssist S
TSKgel CM-2SW
TSK-GEL CM-2SW columns are packed with 5 micron, high efficiency, spherical silica beads which are surface modified with a weak cation exchanger. They are used for the separation and analysis of small proteins, peptides, and other biologically active molecules.
They have a smaller pore size than the TSKgel CM-3SW columns.
|
Product Attributes:
| Matrix: |
spherical silica |
| Particle size (mean): |
5µm |
| Pore size (mean): |
125Å |
| Functional group: |
-CH2COO- |
| Counter ion: |
Na+ |
| pH range: |
2.0 - 7.5 |
Capacity
(mg Hb/mL): |
110 |
| Small ion capacity: |
>0.3meq/mL |
| pKa: |
4.2 |
|
Literature:
Catalog -- Chromatography Catalog, IEC Columns, Overview
Catalog -- Chromatography Catalog, IEC Columns, Cation
Instruction Manual -- Guardgel Kits for TSK-GEL HPLC Columns
Operating Conditions and Specifications
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
07167 |
TSKgel CM-2SW |
5µm |
Stainless Steel |
4.6 |
25 |
07650 |
Guardgel Kit for P/N 07167 |
|
|
|
|
|
|
TSKgel CM-3SW
TSKgel CM-3SW columns are packed with 5 micron, high efficiency, spherical silica beads which are surface modified with a weak cation exchanger. They are used for the separation and analysis of proteins, peptides, and other biologically active molecules.
They have a larger pore size than the TSKgel CM-2SW columns.
|
Product Attributes:
| Matrix: |
spherical silica |
| Particle size (mean): |
10µm |
| Pore size (mean): |
250Å |
| Functional group: |
-CH2COO- |
| Counter ion: |
Na+ |
| pH range: |
2.0 - 7.5 |
Capacity
(mg Hb/mL): |
ND |
| Small ion capacity: |
>0.3meq/mL |
| pKa: |
4.2 |
|
Literature:
Catalog -- Chromatography Catalog, IEC Columns, Overview
Catalog -- Chromatography Catalog, IEC Columns, Cation
Instruction Manual -- Guardgel Kits for TSK-GEL HPLC Columns
Operating Conditions and Specifications
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
07162 |
TSKgel CM-3SW |
10 |
Stainless Steel |
7.5 |
7.5 |
07650 |
Guardgel Kit for P/N 07162 |
|
|
|
|
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TSKgel CM-5PW
TSKgel CM-STAT
TSK-GEL CM-STAT columns are packed with 7 and 10µm hydrophilic non-porous resin particles of which the surface consists of an open access network of multi-layered weak cation exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity, low operating pressure and rapid analysis.
Applications for the TSK-GEL CM-STAT columns include the separation of proteins, protein aggregates, charge isomers of monoclonal antibodies, PEGylated proteins, and peptide digests.
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Product Attributes:
| Matrix: |
hydrophilic polymer |
| Particle size (mean): |
7µm and 10µm |
| Pore size (mean): |
non-porous |
| Functional group: |
carboxymethyl |
| Counter ion: |
Na |
| pH range: |
3.0 - 10.0 |
Static binding
capacity (mg
Lysozyme/mg dry gel): |
ca. 20 (7µm)
ca. 15 (10µm) |
| Small ion capacity: |
100ueq/g dry gel |
| pKa: |
4.9 |
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Literature:
Technical Presentation -- High Speed and High Resolution Cation Exchange Chromatography for Biological Samples on Non-Porous Packings
Operating Conditions and Specifications
Product Overview -- TSK-GEL CM-STAT Cation Exchange Columns
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Applications |
The analysis profiles for five antibodies on a TSKgel CM-STAT column were compared with the profiles obtained on a competitive non-porous cation exchange column. Similar or higher resolution profiles were obtained on the TSKgel CM-STAT column in approximately half the time.
TSKgel CM-STAT, 4.6mm ID x 10cm
ProPac WCX-10, 4mm ID x 25cm
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
21965 |
TSKgel CM-STAT |
10µm |
Stainless Steel |
3 |
3.5 |
21966 |
TSKgel CM-STAT |
7µm |
Stainless Steel |
4.6 |
10 |
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TSKgel OApak-A
TSKgel OApak-A columns are packed with porous hydrophilic polymer beads which have been chemically modified with a weak cation exchanger. They are optimized for the separation and analysis of organic acids by an ion-exclusion mechanism.
This column is to be used in conjunction with the TSKgel OApak-P guard column which has a strong cation exchanger for the removal of dissociated strong acids under the isocratic eluent conditions of 0.75mM H2SO4.
Applications include: organic acids in fruit juices, wine, beer, coffee, and salt solutions. Consult the HPLC Application Database for more information.
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Product Attributes:
| Matrix: |
hydrophilic
polymer |
| Particle size (mean): |
5µm |
| Pore size (mean): |
ND |
| Functional group: |
proprietary |
| Counter ion: |
H+ |
| pH range: |
2.0 - 12.0 |
| Small ion capacity: |
>1.5meq/mL |
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Literature:
Catalog -- Chromatography Catalog, IEC Columns, Overview
Catalog -- Chromatography Catalog, IEC Columns, Cation
Operating Conditions and Specifications
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Applications |
Separation of organic acids commonly found in wine and beer on TSKgel OApak-P and OApak-A
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
16653 |
TSKgel OApak-A |
5µm |
Stainless Steel |
7.8 |
30 |
16654 |
TSKgel OApak-P Guard column |
10µm |
Stainless Steel |
6 |
4 |
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TSKgel SCX
TSKgel SCX columns are packed with porous hydrophilic polymer beads which are surface modified with a strong cation exchanger. They are optimized for the separation and analysis of organic acids, saccharides and alcohols.
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Product Attributes:
| Matrix: |
PS-DVB |
| Particle size (mean): |
5µm |
| Pore size (mean): |
60Å |
| Functional group: |
sulfonic acid |
| Counter ion: |
H+, and Na+ |
| pH range: |
2-12 |
| Capacity |
ND |
| Small ion capacity: |
>1.5 meq/mL |
| pKa: |
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Literature:
Catalog -- Chromatography Catalog, IEC Columns, Overview
Catalog -- Chromatography Catalog, IEC Columns, Cation
Operating Conditions and Specifications (Na+)
Operating Conditions and Specifications (H+)
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Applications |
Separation of saccharide, organic acid and alcohol mixture
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
07156 |
TSKgel SCX (Na+) |
5µm |
Stainless Steel |
6 |
15 |
07158 |
TSKgel SCX (H+) |
5µm |
Stainless Steel |
7.8 |
30 |
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TSKgel SP-2SW
TSKgel SP-2SW columns are packed with 5 micron, high efficiency, spherical silica beads which are surface modified with a strong cation exchanger. They are used for the separation and analysis of small proteins, peptides, nucleosides, and other biologically active molecules.
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Product Attributes:
| Matrix: |
spherical silica |
| Particle size (mean): |
5µm |
| Pore size (mean): |
125 Å |
| Functional group: |
sulfopropyl |
| Counter ion: |
Na+ |
| pH range: |
2.0 - 7.5 |
Capacity
(mg Hb/mL): |
ND |
| Small ion capacity: |
0.3 meq/mL |
| pKa: |
2.2 |
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Literature:
Catalog -- Chromatography Catalog, IEC Columns, Overview
Catalog -- Chromatography Catalog, IEC Columns, Cation
Instruction Manual -- Guardgel Kits for TSK-GEL HPLC Columns
Operating Conditions and Specifications
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Applications |
Separation of nucleosides by ion-exchange chromatography on TSKgel SP-2SW
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
07165 |
TSKgel SP-2SW |
5µm |
Stainless Steel |
4.6 |
25 |
07644 |
Guardgel Kit for P/N 07165 |
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Stainless Steel |
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TSKgel SP-5PW
TSKgel SP-5PW columns are packed with porous hydrophilic polymer beads which are surface modified with a strong cation exchanger. They are used for the separation and analysis of proteins, peptides, and other biologically active molecules.
TSK-GEL SP-5PW columns have the same backbone chemistry and selectivity as the bulk process scale resins Toyopearl SP-650 and TSK-GEL SP-5PW.
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Product Attributes:
| Matrix: |
hydroxylated methacrylic polymer |
| Particle size (mean): |
10, 13, 20µm |
| Pore size (mean): |
1000Å |
| Functional group: |
-(CH2)3SO3- |
| Counter ion: |
Na+ |
| pH range: |
2.0 -12 |
Capacity
(mg Hb/mL): |
40 |
| Small ion capacity: |
>0.1 meq/mL |
| pKa: |
2.3 |
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Literature:
Catalog -- Chromatography Catalog, IEC Columns, Overview
Catalog -- Chromatography Catalog, IEC Columns, Cation
Instruction Manual -- Guardgel Kits for TSK-GEL HPLC Columns
Operating Conditions and Specifications
Separation Report -- Separation of Monoclonal Antibodies Using TSK-GEL HPLC Columns
Separation Report -- Application of TSK-GEL Semi-Micro Columns for High-Sensitivity Analyses
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Applications |
Selectivity of TSK-GEL strong and weak cation exchangers
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
18758 |
TSKgel SP-5PW |
10µm |
Stainless Steel |
2 |
7.5 |
42153 |
Guard Cartridge 3pk for P/N 18758 |
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Stainless Steel |
2 |
1 |
19308 |
Guard Cartridge Holder for P/N 42153 |
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Stainless Steel |
2 |
1 |
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TSKgel SP-5PW |
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Stainless Steel |
7.5 |
7.5 |
07211 |
Guardgel Kit for P/N 07161 |
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Stainless Steel |
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07575 |
TSKgel SP-5PW |
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Stainless Steel |
21.5 |
15 |
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Guardgel Kit for P/N 07575 |
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Stainless Steel |
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07934 |
TSKgel SP-5PW |
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Stainless Steel |
55 |
20 |
07932 |
Guard Column for for P/N 07934 |
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Stainless Steel |
45 |
5 |
13062 |
TSKgel SP-5PW |
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Glass |
5 |
5 |
08803 |
TSKgel SP-5PW |
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Glass |
8 |
7.5 |
08807 |
Guardgel Kit for for P/N 08803 |
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Glass |
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14017 |
TSKgel SP-5PW |
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Glass |
20 |
15 |
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TSKgel SP-NPR
TSKgel SP-STAT
TSK-GEL SP-STAT columns are packed with 7 and 10µm hydrophilic non-porous resin particles of which the surface consists of an open access network of multi-layered cation exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity and a low operating pressure.
Applications for the TSK-GEL SP-STAT columns include the separation of proteins, protein aggregates, charge isomers of monoclonal antibodies, PEGylated proteins, and peptide digests.
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Product Attributes:
| Matrix: |
hydrophilic polymer |
| Particle size (mean): |
7µm and 10µm |
| Pore size (mean): |
non-porous |
| Functional group: |
sulfopropyl |
| Counter ion: |
Na |
| pH range: |
3.0 - 10.0 |
Static binding
capacity (mg
Lysozyme/mg dry gel): |
ca. 15 (7µm)
ca. 10 (10µm) |
| Small ion capacity: |
23ueq/g dry gel |
| pKa: |
4.0 |
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Literature:
Technical Presentation -- High Speed and High Resolution Cation Exchange Chromatography for Biological Samples on Non-Porous Packings
Operating Conditions and Specifications
Product Overview -- TSK-GEL SP-STAT Cation Exchange Columns
|
Applications |
Separation of nucleosides by ion-exchange chromatography on TSKgel SP-2SW
Conversion ratio of peak area
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
21963 |
TSKgel SP-STAT |
10µm |
Stainless Steel |
3 |
3.5 |
21964 |
TSKgel SP-STAT |
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Stainless Steel |
4.6 |
10 |
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TSK-GEL® DNA-STAT columns are packed with 5µm hydrophilic non-porous resin particles of which the surface consists of an open access network of multi-layered anion exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity, low operating pressure and rapid analysis.
Applications for the TSK-GEL DNA-STAT columns include the separation of: DNA fragments, nucleic acids and nucleotides.
TSK-GEL® Q-STAT columns are packed with 7 and 10µm hydrophilic non-porous resin particles of which the surface consists of an open access network of multi-layered anion exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity and a low operating pressure, making these columns suitable for all HPLC and FPLC systems.
Applications for the TSK-GEL Q-STAT columns include the separation of: proteins, peptides, low molecular weight nucleic acids, aggregates and charge isomers of monoclonal antibodies, PEGylated proteins, oligo DNA, and siRNA.
TSK-GEL® SP-STAT columns are packed with 7 and 10µm hydrophilic non-porous resin particles of which the surface consists of an open access network of multi-layered cation exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity and a low operating pressure.
Applications for the TSK-GEL SP-STAT columns include the separation of proteins, protein aggregates, charge isomers of monoclonal antibodies, PEGylated proteins, and peptide digests.
TSK-GEL® CM-STAT columns are packed with 7 and 10µm hydrophilic non-porous resin particles of which the surface consists of an open access network of multi-layered weak cation exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity, low operating pressure and rapid analysis.
Applications for the TSK-GEL CM-STAT columns include the separation of proteins, protein aggregates, charge isomers of monoclonal antibodies, PEGylated proteins, and peptide digests.
For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.
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