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TSK-GEL kolonner I Resiner I Process-development kolonner I LabPak Media I Kataloger I Alle Tosoh produkter
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The silica-based TSK-GEL reversed phase product line consists of eleven stationary phases designed for the analysis of low molecular weight analytes such as:
Depending on their molecular weight, peptides may be analyzed with the same silica-based RPC columns, while proteins require a larger pore size column (200-300 angstrom ). TSK-GEL silica packings are spherical with uniform pore sizes of 100, 140, 150, or 250 angstrom bonded with a monomeric or polymeric layer of octadecyl, octyl, cyano, trimethylsilyl or phenyl groups. Several of the stationary phases are subsequently capped with trimethylsilyl groups by a proprietary method that deactivates the unreacted but accessible silanol groups.
80Å Pore Size Monomeric RP Silica
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Ordering information:
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Literature:
Brochure -- Reversed Phase and HILIC TSK-GEL HPLC Columns
Catalog -- Chromatography Catalog, Traditional Reversed Phase Columns
Operating Conditions and Specifications
Application |
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
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TSKgel Octyl-80Ts has a lower carbon load and hydrophobicity than the corresponding ODS products. The C8 alkyl ligand provides a unique selectivity for the analysis of low MW pharmaceuticals, bases, nucleosides and nucleotides. Featuring high purity, metal free silica and a proprietary technique for complete endcapping of residual silanol groups, TSKgel Octyl-80Ts columns reduce tailing when analyzing basic compounds. Common applications include purity checks and peptide mapping. Bonded phase pore size is indicated by the number in the product description, in this case TSKgel Octyl-80Ts has 80Å nominal pore size. The pore size of the base silica is 100Å. |
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Literature:
Brochure -- Reversed Phase and HILIC TSK-GEL HPLC Columns
Catalog -- Chromatography Catalog, Traditional Reversed Phase Columns
Operating Conditions and Specifications
Applications |
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
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TSK-GEL ODS-80Ts QA columns were developed specifically for use by QA/QC departments that require highly reproducible separations. These columns are prepared from the same endcapped C18 packing material as TSK-GEL ODS-80Ts columns, but with narrower manufacturing specifications to meet the demand for high reproducibility.
The variation between different lots of TSK-GEL ODS-80Ts QA packing material is minimized by selecting batches of TSK-GEL ODS-80Ts that fall within a very narrow range of specifications, as demonstrated in the table below. In addition, each column must pass demanding specifications for efficiency (N) and peak asymmetry, as are spelled out in the Operating Conditions and Specifications sheet. The end result is TSK-GEL ODS-80Ts QA columns that exhibit an unparalleled level of reproducibility for retention, selectivity (k´), efficiency (N), and peak symmetry.
Product Specifications:
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Column classification:
TSK-GEL ODS-80Ts QA columns were submitted to several characterization tests to determine the level of hydrophobic retention, steric selectivity and retention of basic compounds. The results of the characterization tests were used to establish specifications listed in the Table below.
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1. 80% CH3OH/20% H2O, T=40°C
2. 10% ACN/90% 20mmol/L Na2HPO4 (pH6.6, adjusted with 20mmol/L NaH2PO4, T=40°C)
3. 7% ACN/93% 20mmol/L H3PO4, T=40°C
Application To demonstrate the lot-to-lot reproducibility that can be expected when using TSK-GEL ODS-80Ts QA columns, the contents of an over-the-counter analgesic pain reliever was analyzed on columns from three different lots of packing material. The results are shown in the Figure to the right and Table below. Excellent reproducibility of retention, peak height, peak area, efficiency and peak shape is evident for all three ingredients. Highly reproducible results can be achieved using the TSK-GEL ODS-80Ts QA columns as shown in this lot-to-lot reproducibility test. |
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Literature:
Brochure -- Reversed Phase and HILIC TSK-GEL HPLC Columns
Operating Conditions and Specifications
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
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100Å Pore Size Monomeric RP Silica
TSKgel ODS-100V and TSKgel ODS-100Z columns incorporate the best-in-class surface properties to limit secondary interactions of basic, acidic and chelating compounds. Take advantage of the benefits of high column efficiency and symmetrical peak shapes to reduce your analysis and method development time! The ultra high purity Type B base silica contains negligible amounts of metal ion impurities. Combined with monomeric bonding chemistry, this silica makes the best general purpose Reversed Phase columns suitable for the most demanding separations in quality control as well as in research and development. |
Highlights
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TSKgel ODS-100V provides longer retention for polar compounds than the higher coverage TSKgel ODS-100Z column. Proprietary monomeric bonded phase chemistry provides complete wetting and retention stability in 100% aqueous mobile phases. Inert silica surface prevents secondary interaction with metal impurities for improved efficiency and peak shape of basic, acidic and chelating compounds.
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Ordering information:
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TSKgel ODS-100Z contains a high density monomeric C18 bonded phase for maximum retention and selectivity of small molecular weight compounds. Exhaustive endcapping prevents secondary interaction with residual silanol groups. This general purpose column is the workhorse for analysis of small molecular weight compounds in life science applications. Lot-to-lot reproducibility stands out. |
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2μm Super Series RP Silica (110Å)
TSK-GEL Super-ODS, Super-Octyl and Super-Phenyl columns are prepared from spherical 2µm silica particles with 140Å pore diameter. The silica beads in the columns are extremely uniform in size which provides column efficiencies of more than 160,000 plates per meter, yet have a pressure drop comparable to columns packed with 3µm particles. The main advantages of the Super Series columns are savings in time and solvent usage, in addition to the improved quantitation resulting from reduced band broadening. Caution: The silica particles in Super Series columns have a relatively small pore volume, which results in shorter retention times than obtained on most other reversed phase columns. For instance, to achieve similar retention times as obtained on TSKgel ODS-100V, the percentage organic solvent in the mobile phase has to be reduced by about 20-30% on TSKgel Super-ODS columns. |
Highlights
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TSKgel Super-ODS columns are packed with monodispersed 2µm* spherical silica particles covalently bonded with octadecyl groups. The small particle size makes the Super series the highest efficiency reversed phase columns in the TSK-GEL product line. The monodispersed packing generates operational back pressures more typical of larger particles allowing the use of higher flow rates than other 2 micron packings TSK-GEL Super-ODS is an excellent choice for small peptides, amino acids, tryptic digests, nucleotides, pharmaceutical molecules and food/beverage samples. |
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Literature:
Brochure -- Reversed Phase and HILIC TSK-GEL HPLC Columns
Catalog -- Chromatography Catalog, Traditional Reversed Phase Columns
Operating Conditions and Specifications
Product Overview -- TSK-GEL Super Series Reversed Phase Columns
Applications |
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
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TSKgel Super-Octyl columns are packed with monodispersed 2µm* spherical silica particles covalently bonded with octyl groups. The small particle size makes the Super series the highest efficiency reversed phase columns in the TSK-GEL product line. The monodispersed packing generates operational back pressures more typical of larger particles allowing the use of higher flow rates than other 2 micron packings and offers less hydrophobicity than TSKgel Super-ODS. TSK-GEL Super-Octyl is an excellent choice for peptides, proteins, amino acids, tryptic digests, nucleotides, pharmaceutical molecules and food/beverage samples |
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Literature:
Brochure -- Reversed Phase and HILIC TSK-GEL HPLC Columns
Catalog -- Chromatography Catalog, Traditional Reversed Phase Columns
Operating Conditions and Specifications
Product Overview -- TSK-GEL Super Series Reversed Phase Columns
Applications |
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
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TSKgel Super-Phenyl columns are packed with monodispersed 2µm* spherical silica particles covalently bonded with phenyl groups. The small particle size makes the Super series the highest efficiency reversed phase columns in the TSK-GEL product line. The monodispersed packing generates operational back pressures more typical of larger particles allowing the use of higher flow rates than other 2 micron packings and offers less hydrophobicity than TSKgel Super-Octyl and TSKgel Super-ODS.
TSK-GEL Super-Phenyl is an excellent choice for peptides, proteins, amino acids, tryptic digests, nucleotides, pharmaceutical molecules and food/beverage samples.
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Literature:
Brochure -- Reversed Phase and HILIC TSK-GEL HPLC Columns
Catalog -- Chromatography Catalog, Traditional Reversed Phase Columns
Operating Conditions and Specifications
Product Overview -- TSK-GEL Super Series Reversed Phase Columns
Applications |
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
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Specialty RP Columns
| TSKgel ODS-120A | TSKgel ODS-120T | TSKgel OligoDNA-RP | TSKgel TMS-250 |
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TSKgel ODS-120A columns use a 150Å pore size silica base support. The silica's exclusion limit of 10,000 Da makes them a good choice for the reversed phase chromatography of peptides, small proteins, and environmental samples such as poly-aromatic hydrocarbons. Bonded phase pore size is indicated by the number in the product description, in this case TSKgel ODS-120A has 120Å nominal pore size. |
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Literature:
Brochure -- Reversed Phase and HILIC TSK-GEL HPLC Columns
Catalog -- Chromatography Catalog, Reversed Phase Columns
Operating Conditions and Specifications
Applications |
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
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TSKgel ODS-120T columns use a 150Å pore size silica base support. The silica's exclusion limit of 10,000 Da makes them a good choice for the reversed phase chromatography of peptides, small proteins, and small molecular weight compounds in organic and environmental samples. Bonded phase pore size is indicated by the number in the product description, in this case TSKgel ODS-120T has 120Å nominal pore size. |
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Literature:
Brochure -- Reversed Phase and HILIC TSK-GEL HPLC Columns
Catalog -- Chromatography Catalog, Traditional Reversed Phase Columns
Operating Conditions and Specifications
Separation Report -- Application of TSK-GEL Semi-Micro Columns for High-Sensitivity Analyses
Applications |
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
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Specifically designed for purification of oligonucleotides, RNA and DNA fragments (up to 500mer), TSKgel OligoDNA RP can provide excellent separations of samples with very similar sequences. The 250Å pore size provides excellent kinetics for molecules with helix shape structures processing large radii of gyration. The 5 micron particle size provides a minumum of 7,000 plates per 15 centimeter column. |
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Literature:
Brochure -- Reversed Phase and HILIC TSK-GEL HPLC Columns
Catalog -- Chromatography Catalog, Traditional Reversed Phase Columns
Operating Conditions and Specifications
Applications |
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
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Featuring trimethylsilane groups covalently attached to large 250Å pores, TSKgel TMS-250 is an excellent choice for analysis of larger proteins by reversed phase HPLC. Due to the low hydrophobicity of the ligand, excellent recoveries are common even when used with large proteins. Proteins such as adolase (158kDa) exhibit sharp peaks relative to wide pore C18 columns.
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Literature:
Brochure -- Reversed Phase and HILIC TSK-GEL HPLC Columns
Catalog -- Chromatography Catalog, Traditional Reversed Phase Columns
Operating Conditions and Specifications
Applications |
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
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High Throughput RP Silica
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TSKgel ODS-140HTP columns provide high resolution and short analyses time at moderate pressures, enabling high throughput separations. The polylayer bonding chemistry of the 2.3µm particle size of these columns results in highly efficient and durable columns. The lower pressure drop reduces the burden on the hardware, allowing the TSK-GEL ODS-140HTP columns to be used with either UPLC® (up to 9000psi) or conventional HPLC systems.
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Literature:
Brochure -- Reversed Phase and HILIC TSK-GEL HPLC Columns
Catalog -- Chromatography Catalog, Reversed Phase Columns
Operating Conditions and Specifications
Product Overview -- TSK-GEL ODS-140HTP Columns
Performance Data |
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
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For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.