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TSK-GEL GPC columns are available in conventional 7.8mmID x 30cm Hxl and Hhr columns and in high efficiency, high throughput 6mmID x 15cm SuperH and SuperHZ columns. How the product lines compare to one another is shown below.
Comparison of TSK-GEL H-type resins
Series Type |
SuperHZ |
Hxl |
SuperH |
Hhr |
Application focus |
High-throughput polymer analysis with ultra low polymer adsorption, limited solvent compatibility range.
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Conventional polymer analysis with ultra low polymer adsorption, limited solvent compatibility range
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High-throughput polymer analysis with expanded solvent compatibility range.
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Conventional polymer analysis with expanded solvent compatibility range.
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Particle size |
3, 5, and 10 micron, depending on pore size
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5 and 9 micron, depending on pore size
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3 and 5 micron, depending on pore size
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Theoretical plates 1 |
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Maximum temperature |
G1000--G3000, 60°C
G4000--Mixed, 80°C
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G1000--G3000, 60°C
G4000--Mixed, 80°C
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Standard shipping solvent |
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THF can be switched to |
see table 1 (below) and note #3 for dichloromethane and dichloroethane
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Other shipping solvents available? |
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Number of solvent substitutions |
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Solvent exchange instructions |
Linear gradient with a 2%/min rate of change with a flow rate of <0.25mL/min.
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Linear gradient with a 2%/min rate of change with a flow rate of <0.5mL/min.
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Linear gradient with a 2%/min rate of change according to flow rates in table 2 (below).
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1) Theoretical plates listed are based on smallest particle size listed.
2) High-temperature columns (HT) are shipped with ODCB (o-dichlorobenzene) as standard shipping solvent.
3) Dichloromethane and dichloroethane are not available for G1000 pore size columns.
4) See Table 1 (below) for available shipping solvents
5) After switching to a very polar solvent such as acetone, switching back to a nonpolar solvent is not recommended.
Table 1: Solvent compatibility for TSK-GEL H-type columns
The column shipping solvent limits the types of solvents that can be used with each column type.
Column type |
Shipping solvent* |
Can be replaced with |
SuperHZ and Hxl1
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benzene, chloroform, toluene, xylene, dichloromethane, dichloroethane
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carbon tetrachloride5, o-dichlorobenzene, dimethylformamide, dodecane, dimethyl sulfoxide, dioxane, ethylacetate, FC-113, hexane, pyridine, hexafluoroisopropanol/chloroform, methyl ethyl ketone, quinoline, cyclohexane
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m-cresol in chloroform, up to 10% hexafluoroisopropanol/chloroform
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dimethyl sulfoxide, dioxane, tetrahydrofuran, toluene
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1-chloronaphthalene, trichlorobenzene
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SuperH and Hhr2 |
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acetone, ethanol, quinoline, benzene, o-dichlorobenzene, ethyl acetate, dodecane, FC-113, carbon tetrachloride5, dichloromethane, dichloroethane, trichloroethane, n-hexane, cyclohexane, xylene, tetrahydrofuran, chloroform, 1,4-dioxane, hexafluoroisopropanol, toluene, 1-chloronaphthalene, dimethylaceto acetamide, methyl ethyl ketone, trichlorobenzene, m-cresol, dimethylformamide, methylpyrrolidone, o-chlorophenol/chloroform, dimethyl sulfoxide, pyridine
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SuperMultiporeHZ |
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Cannot be replaced. SuperMultiporeHZ columns can be used only in Tetrahydrofuran
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Notes:
1) In case of SuperHZ and Hxl, keep flow rate below <0.5mL/min during solvent change. Solvent can be changed one way/one time only.
2) In case of SuperH and Hhr, see Table 2 below for appropriate flow rates for solvent exchange. After switching to a very polar solvent, switching to a nonpolar solvent is not recommended.
3) All TSK-GEL Hxl, Hhr, SuperHZ, SuperH, SuperMultipore, and GMH analytical columns are shipped containing tetrahydrofuran (THF), except GMH-HT columns, which contain o-dichlorobenzene (ODCB).
4) THF in G1000Hxl columns cannot be replaced with dichloromethane or dichloroethane.
5) Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.
* 100% methanol cannot be used with H-type columns; use this solvent with TSK-GEL SW-type or Alpha-type columns.
** TSK-GEL H columns may be specially ordered with this shipping solvent.
Table 2: Recommended flow rates (mL/min) for solvent exchange in TSK-GEL SuperH and Hhr columns
Solvent |
SuperH (6.0mmIDx15cm) |
Hhr (7.8mmIDx30cm) |
n-Hexane |
0.5 |
0.9 |
methyl ethyl ketone |
0.4 |
0.7 |
dichloromethane, ethyl acetate |
0.35 |
0.6 |
toluene, chloroform |
0.3 |
0.5 |
dimethylformamide |
0.2 |
0.4 |
carbon tetrachloride, pyridine |
0.15 |
0.3 |
dimethyl sulfoxide, dioxane, ethanol,
N-methylpyrrolidone, o-dichlorobenzene |
0.1 |
0.2 |
quinoline, hexafluoroisopropanol,
1-chloronaphthalene |
0.05 |
0.1 |
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For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.
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