|
|
TSK-GEL GPC columns for organic-soluble polymers are packed with spherical particles composed of polystyrene crosslinked with divinylbenzene (PS-DVB). Main differences between the available product lines are highlighted in the Table below.
Tosoh redefines GPC calibration.
Tosoh's innovative multi-pore technology produces particles that contain a broad range of pore sizes. Columns with an extended linear calibration curve can now be prepared without mixing particles of different pore sizes.
TSK-GEL SuperH and TSK-GEL SuperHZ columns were developed for High-Throughput GPC applications. Both product lines feature particle sizes down to 3μm, a column length of 15cm and column diameters of 6mm ID and 4.6mm ID (SuperHZ only).
TSK-GEL Hxl and TSK-GEL Hhr columns are conventional GPC columns of 7.8mm ID x 30cm, while particles sizes are 5µm and larger for the larger pore size columns. |
Highlights
-
Tosoh manufactures two distinct product lines for GPC of organic-soluble polymers. While each line is based on particles prepared from polystyrene crosslinked with divinylbenzene, they differ in:
-
TSK-GEL Hxl-type, SuperHZ-type, and SuperMultiporeHZ-type columns are most inert to sample adsorption.
- TSK-GEL Hhr- and SuperH-type columns provide minimal shrinking and swelling of the column bed when changing solvents and allow operation up to 140°C.
- Small particle, narrow bore SuperMultiporeHZ, SuperHZ and SuperH columns provide high efficiency, short analysis times and operating cost.
- Except for SuperMultiporeHZ columns, all other TSK-GEL H-type column lines contain at least five different pore sizes.
- Each product line contains columns for the analysis of oligomers to polymers containing molecular weights as high as as 4 x 10^8Da (estimated).
-
All product lines contain a number of linear columns that are prepared by blending particles with different pore sizes.
|
Table: Distinguishing features of TSK-GEL H-type GPC column lines
Hxl and SuperHZ |
|
|
- Ultra-low adsorption columns with limited solvent range.
- Use SuperHZ for highest resolution and throughput.
- Use Hxl if you can live with longer analyis times.
- Max. temp: 60-80°C
|
- More linear than 'linear' mixed-bed columns.
- Improved MW accuracy and precision.
- Like SuperHZ and Hxl lines in terms of surface chemistry and temp. range.
- Like SuperHZ columns in terms of resolution and speed.
|
- Low adsorption columns with expanded solvent range.
- SuperH for high throughput and high resolution GPC.
- Use Hhr if you do not have a need to save time.
- Max. temp:140°C
|
|
TSK-GEL Hxl-type Columns for Reduced Polymer Adsorption and Limited Solvent Range
The TSK-GEL Hxl column lines consists of eight columns with different pore sizes, TSKgel G1000Hxl through TSKgel G7000Hxl, and three columns with an extended linear range of the calibration curve, TSKgel GMHxl, TSKgel GMHxl-L and TSKgel MultiporeHxl-M. The calibration curves are shown to the right.
Main characteristics
- Ultra-low sample adsorption, i.e., the columns show true size exclusion behavior for most polymers
- Maximum operating temperature for Hxl-type columns is 60°C for G1000Hxl, G2000Hxl, G2500Hxl and G3000Hxl, and 80°C for the remaining columns in the product line. See Hhr-type columns for operating temperatures of 140°C.
- Limited solvent range. The columns are shipped in THF, which can be exchanged for a limited number of organic solvents (see Table below)
- Hxl-type columns are conventional GPC columns with column dimensions of 7.8mm ID x 30cm. Most HPLC systems do not require optimization of extra-column bandbroadening effects when working with conventional GPC columns.
|
Calibration curves for TSK-GEL Hxl columns with polystyrene standards
|
|
Solvent compatibility for TSK-GEL Hxl (and SuperHZ) columns
The column shipping solvent limits the types of solvents that can be used with each column type.
Column type |
Shipping solvent* |
Can be replaced with |
Hxl1 and SuperHZ
|
|
benzene, chloroform, toluene, xylene, dichloromethane, dichloroethane
|
|
carbon tetrachloride4, o-dichlorobenzene, dimethylformamide, dodecane, dimethyl sulfoxide, dioxane, ethylacetate, FC-113, hexane, pyridine, hexafluoroisopropanol/chloroform, methyl ethyl ketone, quinoline, cyclohexane
|
|
m-cresol in chloroform, up to 10% hexafluoroisopropanol in chloroform
|
|
dimethyl sulfoxide, dioxane, tetrahydrofuran, toluene
|
|
1-chloronaphthalene, trichlorobenzene
|
|
- In case of SuperHZ and Hxl, keep flow rate below <0.5mL/min during solvent change. Solvent can be changed one way/one time only.
- All TSK-GEL Hxl, Hhr, SuperHZ, SuperH and GMH analytical columns are shipped containing tetrahydrofuran (THF), except GMH-HT columns, which contain o-dichlorobenzene.
- THF in G1000Hxl columns cannot be replaced with dichloromethane or dichloroethane.
- Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.
* 100% methanol cannot be used with H-type columns; use this solvent with TSK-GEL SW-type or Alpha-type columns.
** TSK-GEL H columns may be specially ordered with this shipping solvent.
Recommended solvents/Applications for TSK-GEL H-type columns
Recommended solvent |
Application |
THF |
polystyrene, epoxy resin, phenoxy resin, polycarbonate, polyisoprene, polyvinyl acetate, polyvinyl chloride, monoglycerides, fatty acids, poly(butadiene), polybutadiene, poly(methyl methacrylate), poly(styrene-butadiene),
poly(styrene-acrylonitrile)
|
N,N-Dimethylformamide (DMF) +/- 5mM LiBr |
polyvinyl chloride, polyvinyl fluoride, urea resins, polyurethane, polystyrene, polyester, polyimido ether, polyimido ester, polyphenol (aqueous solution), polyacrylonitrile
|
o-Dichlorobenzene (ODCB) |
polyethylene, polypropylene
|
Chloroform |
polycarboxylic ether, acrylic resin, epoxy resin, polystyrene
|
m-Cresol/Chloroform |
nylon, polyester, polyamide, poly(ethylene terephthalate)
|
Toluene |
polybutadiene, polysiloxane
|
|
Columns with linear calibration curves
Two of the linear columns are so-called mixed-bed columns, in which particles with different pore sizes are blended to provide an extended linear calibration curve. The remaining column is a so-called multi-pore column, in which each particle contains a range of pore sizes that provide a linear calibration curve. The innovative multi-pore approach, pioneered by Tosoh, is a synthetic chemistry answer to the question of how to obtain a column with an extended linear calibration curve, while mixed-bed columns represent a mechanical way of obtaining a linear calibration curve. In general, Multipore columns have a smoother, more linear, calibration curve. See the product page for Multipore Hxl-M for the principles behind mixed-bed and multi-pore columns.
TSKgel G1000Hxl
TSK-GEL G1000Hxl columns are packed with rigid, porous, polymer beads. G1000Hxl columns are applied to the non-aqueous separation of small molecules and oligomers less than 1,000 Da.
As the number of solvents that can be exchanged directly with the shipping solvent (THF) is limited, use TSK-GEL Hhr-type columns when a broader range of solvents is needed.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
15Å |
| Exclusion limit: |
1,000 Da |
| Max. temp (C°) |
60 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- Principles and Applications of High Performance GPC Columns:TSK-GEL Hxl Type Columns for Oligomer Analysis
|
TSKgel Hxl Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
16131 |
TSKgel G1000Hxl |
5μm |
Stainless Steel |
7.8 |
30 |
07113 |
Guard Column for P/N 16131 |
|
Stainless Steel |
6 |
4 |
|
|
TSKgel G2000Hxl
TSK-GEL G2000Hxl columns are packed with rigid, porous, polymer beads. They are applied to the non-aqueous separation of polymers with molecular weights of less than 10,000 Da.
As the number of solvents that can be exchanged directly with the shipping solvent (THF) is limited, use TSK-GEL Hhr-type columns when a broader range of solvents is needed.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore Size (mean): |
20Å |
| Exclusion Limit: |
10,000 Da |
| Max. temp (C°) |
60 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- Principles and Applications of High Performance GPC Columns:TSK-GEL Hxl Type Columns for Oligomer Analysis
|
TSKgel Hxl Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
16134 |
TSKgel G2000Hxl |
5μm |
Stainless Steel |
7.8 |
30 |
07113 |
Guard Column for P/N 16134 |
|
Stainless Steel |
6 |
4 |
|
|
TSKgel G2500Hxl
|
TSK-GEL G2500Hxl columns are packed with rigid, porous, polymer beads. They are applied to the non-aqueous separation of oligomers and polymers of less than 20,000 Da. As the number of solvents that can be exchanged directly with the shipping solvent (THF) is limited, use TSK-GEL Hhr-type columns when a broader range of solvents is needed.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
30Å |
| Exclusion limit: |
20,000 Da |
| Max. temp. (°C): |
60 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- Principles and Applications of High Performance GPC Columns:TSK-GEL Hxl Type Columns for Oligomer Analysis
|
TSKgel Hxl Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
16135 |
TSKgel G2500Hxl |
5μm |
Stainless Steel |
7.8 |
30 |
07113 |
Guard Column for P/N 16135 |
|
Stainless Steel |
4 |
6 |
|
|
TSKgel G3000Hxl
TSK-GEL G3000Hxl columns are packed with rigid, porous, polymer beads. They are applied to the non-aqueous separation of polymers with molecular weights of less than 60,000 Da.
As the number of solvents that can be exchanged directly with the shipping solvent (THF) is limited, use TSK-GEL Hhr-type columns when a broader range of solvents is needed.
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
16136 |
TSKgel TSKgel G3000Hxl |
5µm |
Stainless Steel |
7.8 |
30 |
07113 |
Guard Column for P/N 16136 |
|
Stainless Steel |
6 |
4 |
|
TSKgel G4000Hxl
TSK-GEL G4000Hxl columns are packed with rigid, porous, polymer beads. They are applied to the non-aqueous separation of polymers with molecular weights of less than 400,000 Da.
As the number of solvents that can be exchanged directly with the shipping solvent (THF) is limited, use TSK-GEL Hhr-type columns when a broader range of solvents is needed.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
200Å |
| Exclusion limit: |
400,000 Da |
| Max. temp.(°C): |
80 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- Principles and Applications of High Performance GPC Columns:TSK-GEL Hxl Type Columns for Oligomer Analysis
|
TSKgel Hxl Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
16137 |
TSKgel G4000Hxl |
5μm |
Stainless Steel |
7.8 |
30 |
07113 |
Guard column for P/N 16137 |
|
Stainless Steel |
6 |
4 |
|
|
TSKgel G5000Hxl
TSK-GEL G5000Hxl columns are packed with rigid, porous, polymer beads. They are applied to the non-aqueous separation of polymers with molecular weights of less than 4,000,000 Da.
As the number of solvents that can be exchanged directly with the shipping solvent (THF) is limited, use TSK-GEL Hhr-type columns when a broader range of solvents is needed.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
9µm |
| Pore size (mean): |
650Å |
| Exclusion limit: |
4,000,000 Da |
| Max. temp.(°C) |
80 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- Principles and Applications of High Performance GPC Columns:TSK-GEL Hxl Type Columns for Oligomer Analysis
|
TSKgel Hxl Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
16138 |
TSKgel G5000Hxl |
9μm |
Stainless Steel |
7.8 |
30 |
13727 |
Guard column for P/N 16138 |
|
Stainless Steel |
6 |
4 |
|
|
TSKgel G6000Hxl
TSK-GEL G6000Hxl columns are packed with rigid, porous, polymer beads. They are applied to the non-aqueous separation of polymers with molecular weights of less than 40,000,000 Da.
As the number of solvents that can be exchanged directly with the shipping solvent (THF) is limited, use TSK-GEL Hhr-type columns when a broader range of solvents is needed.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
9µm |
| Pore size (mean): |
ND |
| Exclusion limit (est.): |
40,000,000 Da |
| Max. temp. (°C): |
80 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- Principles and Applications of High Performance GPC Columns:TSK-GEL Hxl Type Columns for Oligomer Analysis
|
TSKgel Hxl Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
16139 |
TSKgel G6000Hxl |
9μm |
Stainless Steel |
7.8 |
30 |
13727 |
Guard Column for P/N 16139 |
|
Stainless Steel |
6 |
4 |
|
|
TSKgel G7000Hxl
TSK-GEL G7000Hxl columns are packed with rigid, porous, polymer beads. They are applied to the non-aqueous separation of very large polymers with molecular weights up to 400,000,000 Da.
As the number of solvents that can be exchanged directly with the shipping solvent (THF) is limited, use TSK-GEL Hhr-type columns when a broader range of solvents is needed.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
9µm |
| Pore size (mean): |
ND |
| Exclusion limit (est.): |
400x10^6 |
| Max. temp. (°C): |
80 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- Principles and Applications of High Performance GPC Columns:TSK-GEL Hxl Type Columns for Oligomer Analysis
|
TSKgel Hxl Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
16140 |
TSKgel G7000Hxl |
9μm |
Stainless Steel |
7.8 |
30 |
13727 |
Guard Column for P/N 16140 |
|
Stainless Steel |
6 |
4 |
|
|
TSKgel GMHxl mixed-bed
TSK-GEL GMHxl mixed-bed columns are packed with a mixture of rigid, polymer beads, varying in average pore size, for the analysis of organic-soluble industrial polymers. Mixed bed, or linear columns, are used when a broad range of polymer molecular weights is expected in the sample. TSKgel GMHxl provides high resolution of polymers with a molecular weight up to several million Da.
The shipping solvent THF can be replaced by benzene, chloroform, toluene, and xylene, but not with dichloromethane and dichloroethane. As the number of solvents that can be exchanged directly with the shipping solvent is limited, use TSK-GEL Hhr-type columns when a broader range of organic solvents is needed.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
9µm |
| Pore size (mean): |
mixed pore sizes |
| Exclusion limit (est.): |
4x10^6 Da |
| Max. temp. (°C): |
80 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- Principles and Applications of High Performance GPC Columns:TSK-GEL Hxl Type Columns for Oligomer Analysis
|
TSKgel Hxl Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
16141 |
TSKgel GMHxl, mixed bed |
9µm |
Stainless Steel |
7.8 |
30 |
13727 |
Guard Column for P/N 16141 |
|
Stainless Steel |
6 |
4 |
|
TSKgel GMHxl-HT
TSK-GEL GMHxl-HT columns are packed with a mixture of rigid, polymer beads, varying in average pore size, for the analysis of organic-soluble industrial polymers. Mixed bed or linear columns are used when a broad range of polymer molecular weights is expected in the sample. TSKgel GMHxl-HT provides high resolution of polymers with a molecular weight up to several million Da. The column can be operated at a temperature of 140°C.
The shipping sovent o-dichlorobenzene can be replaced by 1-chloronaphthalene and trichlorobenzene. As the number of solvents that can be exchanged directly with the shipping solvent is limited, use TSK-GEL Hhr-type columns when a broader range of organic solvents is needed.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
13µm |
| Pore size (mean): |
mixed pore sizes |
| Exclusion limit (est.): |
400,000,000Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- Principles and Applications of High Performance GPC Columns:TSK-GEL Hxl Type Columns for Oligomer Analysis |
TSKgel Hxl Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
07112 |
TSKgel GMHxl-HT, mixed bed |
13µm |
Stainless Steel |
7.8 |
30 |
18397 |
Guard Column for P/N 17112 |
|
Stainless Steel |
7.5 |
7.5 |
|
TSKgel GMHxl-L mixed-bed
TSK-GEL GMHxl-L mixed-bed columns are packed with a mixture of rigid, polymer beads, varying in average pore size, for the analysis of organic-soluble industrial polymers. Mixed bed or linear columns are used when a broad range of polymer molecular weights is expected in the sample. This column gives high resolution of polymers with a molecular weight up to about 100,000 Da, although the exlusion limit is higher as can be seen from the Figure showing the calibration curves of Hxl columns.
The shipping sovent THF can be replaced by benzene, chloroform, toluene and xylene, but not with dichloromethane and dichloroethane. As the number of solvents that can be exchanged directly with the shipping solvent is limited, use TSK-GEL Hhr-type columns when a broader range of organic solvents is needed
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
mixed pore sizes |
| Exclusion limit: |
4x10^6 Da |
| Max. temp. (°C): |
80 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- Principles and Applications of High Performance GPC Columns:TSK-GEL Hxl Type Columns for Oligomer Analysis
|
TSKgel Hxl Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
16652 |
TSKgel GMHxl-L |
5μm |
Stainless Steel |
7.8 |
30 |
07113 |
Guard column for P/N 16652 |
|
Stainless Steel |
6 |
4 |
|
|
TSKgel MultiporeHxl-M
TSK-GEL MultiporeHxl-M columns are packed with rigid, polymer beads that have a large pore size distribution within each particle, for the analysis of organic-soluble industrial polymers. Multipore linear columns are used when a broad range of polymer molecular weights is expected in the sample. They provide a smoother calibration curve than a mixed-bed linear column. TSKgel MultiporeHxl-M provides high resolution of polymers with a molecular weight up to several million Da.
The shipping sovent THF can be replaced by benzene, chloroform, toluene and xylene, but not with dichloromethane and dichloroethane. As the number of solvents that can be exchanged directly with the shipping solvent is limited, use TSK-GEL Hhr-type columns when a broader range of organic solvents is needed.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
each particle contains broad distribution of pore sizes |
| Exclusion limit (est.): |
500 - 2x10^6 Da |
| Max. temp. (°C): |
60 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- Principles and Applications of High Performance GPC Columns:TSK-GEL Hxl Type Columns for Oligomer Analysis
|
TSKgel Hxl Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
18403 |
TSKgel MultiporeHxl-M |
5µm |
Stainless Steel |
7.8 |
30 |
18404 |
Guard Column for P/N 18403 |
|
Stainless Steel |
6 |
4 |
|
TSK-GEL SuperHZ-type Columns for Reduced Polymer Adsorption and Limited Solvent Range
The TSK-GEL SuperHZ column line consists of five columns with different pore sizes, TSKgel SuperHZ1000 through TSKgel SuperHZ4000, and three columns with an extended linear range of the calibration curve, TSKgel SuperHZM-N, TSKgel SuperHZM-M and TSKgel SuperHZM-H. The calibration curves are shown below.
Calibration curves for TSK-GEL SuperHZ columns
|
|
Main characteristics for SuperHZ-type columns are:
- TSK-GEL SuperHZ columns have been developed for High Throughput, High Efficiency GPC applications such as those encountered in combinatorial chemistry experiments.
- Ultra-low sample adsorption, i.e., the columns show true size exclusion behavior for most polymers
- Maximum operating temperature for SuperHZ-type columns is 60°C for SuperHZ1000, SuperHZ2000, SuperHZ2500 and SuperHZ3000, and 80°C for the remaining columns in the product line. See SuperH-type columns for operating temperatures of 140°C.
- Limited solvent range. The columns are shipped in THF, which can be exchanged for a limited number of organic solvents as shown below.
Solvent compatibility for TSK-GEL SuperHZ and Hxl columns
The column shipping solvent limits the types of solvents that can be used with each column type.
Column type |
Shipping solvent* |
Can be replaced with |
SuperHZ and Hxl1
|
|
benzene, chloroform, toluene, xylene, dichloromethane, dichloroethane
|
|
carbon tetrachloride4, o-dichlorobenzene, dimethylformamide, dodecane, dimethyl sulfoxide, dioxane, ethylacetate, FC-113, hexane, pyridine, hexafluoroisopropanol/chloroform, methyl ethyl ketone, quinoline, cyclohexane
|
|
m-cresol in chloroform, up to 10% hexafluoroisopropanol/chloroform
|
|
dimethyl sulfoxide, dioxane, tetrahydrofuran, toluene
|
|
1-chloronaphthalene, trichlorobenzene
|
|
Notes:
1) In case of SuperHZ and Hxl, keep flow rate below <0.5mL/min during solvent change. Solvent can be changed one way/one time only.
2) All TSK-GEL Hxl, Hhr, SuperHZ and SuperH analytical columns are shipped containing tetrahydrofuran (THF), except High Temperature (HT) columns, which contain o-dichlorobenzene.
3) THF in G1000Hxl columns cannot be replaced with dichloromethane or dichloroethane.
4) Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.
* 100% methanol cannot be used with H-type columns; use this solvent with TSK-GEL SW-type or Alpha-type columns.
** TSK-GEL H columns may be specially ordered with this shipping solvent.
Recommended Solvents by Application for TSK-GEL H-type columns
Recommended solvent |
Application |
THF |
polystyrene, epoxy resin, phenoxy resin, polycarbonate, polyisoprene, polyvinyl acetate, polyvinyl chloride, monoglycerides, fatty acids, polybutadiene, poly(methyl methacrylate), poly(styrene-butadiene), poly(styrene-acrylonitrile)
|
N,N-Dimethylformamide (DMF) +/- 5mM LiBr |
polyvinyl chloride, polyvinyl fluoride, urea resins, polyurethane, polystyrene, polyester, polyimido ether, polyimido ester, polyphenol (aqueous solution), polyacrylonitrile
|
o-Dichlorobenzene (ODCB) |
polyethylene, polypropylene
|
Chloroform |
polycarboxylic ether, acrylic resin, epoxy resin, polystyrene
|
m-Cresol/Chloroform |
nylon, polyseter, polyamide, poly(ethylene terephthalate)
|
Toluene |
polybutadiene, polysiloxane
|
|
Beware of extra-column bandbroadening
Column dimensions are 6mm ID x 15cm and 4.6mmID x 15cm versus 7.8mm ID x 30cm for conventional GPC columns. The smaller column dimensions translate to a reduction of peak volume by a factor of 3.4 (6mm ID) and a factor of 5.8 (4.6mm ID) versus the same component eluting from a corresponding Hxl column. Thus, your HPLC system may require optimization of components that can give rise to extra-column bandbroadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant.
Columns with linear calibration curves
The TSK-GEL SuperHZ product line contains three linear, or mixed-bed columns, in which particles with different pore sizes are blended to provide an extended linear calibration curve. The mixed-bed columns feature increasing linear calibration ranges, from TSKgel SuperHZM-L, SuperHZM-N, SuperHZM-H.
TSKgel SuperHZ1000
TSK-GEL SuperHZ1000 columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of solvent-soluble small molecules and oligomers less than 1,000 Da. The THF shipping solvent may be replaced by a limited number of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
15Å |
| Exclusion limit: |
1000 Da |
| Max. temp. (°C): |
60 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- SuperHZ Series Ultra-High Throughput GPC Columns
|
TSK-GEL SuperHZ Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
19309 |
TSKgel SuperHZ1000 |
3µm |
Stainless Steel |
4.6 |
15 |
19314 |
Guard Column for P/N 19309 |
|
Stainless Steel |
4.6 |
2 |
19302 |
TSKgel SuperHZ1000 |
|
Stainless Steel |
6 |
15 |
19666 |
Guard Column for P/N 19302 |
|
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperHZ2000
TSK-GEL SuperHZ2000 columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of solvent-soluble small molecules and oligomers less than 10,000 Da. The THF shipping solvent may be replaced by a limited number of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
20Å |
| Exclusion limit: |
10,000 Da |
| Max. temp. (°C): |
60 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- SuperHZ Series Ultra-High Throughput GPC Columns
|
TSK-GEL SuperHZ Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
19303 |
TSKgel SuperHZ2000 |
3µm |
Stainless Steel |
6 |
15 |
19666 |
Guard Column for P/N 19303 |
3µm |
Stainless Steel |
4.6 |
3.5 |
19310 |
TSKgel SuperHZ2000 |
3µm |
Stainless Steel |
4.6 |
15 |
19314 |
Guard Column for P/N 19310 |
3µm |
Stainless Steel |
4.6 |
2 |
|
|
TSKgel SuperHZ2500
TSKgel SuperHZ2500 columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of solvent-soluble small molecules and oligomers less than 20,000 Da. The THF shipping solvent may be replaced by a limited number of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
30Å |
| Exclusion limit: |
20,000 Da |
| Max. temp. (°C): |
60 |
| Shipping solvent: |
THF |
|
Literature:
Application Note -- High-Throughput GPC Analysis of Phenolic Resin Using TSK-GEL SuperHZ Columns
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- SuperHZ Series Ultra-High Throughput GPC Columns
|
TSK-GEL SuperHZ Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
19311 |
TSKgel SuperHZ2500 |
3µm |
Stainless Steel |
4.6 |
15 |
19314 |
Guard Column for P/N 19311 |
3µm |
Stainless Steel |
4.6 |
2 |
19304 |
TSKgel SuperHZ2500 |
3µm |
Stainless Steel |
6 |
15 |
19666 |
Guard Column for P/N 19304 |
3µm |
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperHZ3000
TSK-GEL SuperHZ3000 columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of solvent-soluble small molecules and oligomers less than 60,000 Da. The THF shipping solvent may be replaced by a limited number of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
75Å |
| Exclusion limit: |
60,000 Da |
| Max. temp. (°C): |
60 |
| Shipping solvent: |
THF |
|
Literature:
Application Note -- High-Throughput GPC Analysis of Phenolic Resin Using TSK-GEL SuperHZ Columns
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- SuperHZ Series Ultra-High Throughput GPC Columns
|
TSK-GEL SuperHZ Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
19312 |
TSKgel SuperHZ3000 |
3µm |
Stainless Steel |
4.6 |
15 |
19314 |
Guard Column for P/N 19312 |
3µm |
Stainless Steel |
4.6 |
2 |
19305 |
TSKgel SuperHZ3000 |
3µm |
Stainless Steel |
6 |
15 |
19666 |
Guard Column for P/N 19305 |
3µm |
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperHZ4000
TSKgel SuperHZ4000 columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of solvent-soluble small molecules and oligomers less than 60,000 Da. The THF shipping solvent may be replaced by a limited number of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
200Å |
| Exclusion limit: |
4x10^5 Da |
| Max. temp. (°C): |
80 |
| Shipping solvent: |
THF |
|
Literature:
Application Note -- High-Throughput GPC Analysis of Phenolic Resin Using TSK-GEL SuperHZ Columns
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- SuperHZ Series Ultra-High Throughput GPC Columns
|
TSK-GEL SuperHZ Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
19313 |
TSKgel SuperHZ4000 |
3µm |
Stainless Steel |
4.6 |
15 |
19314 |
Guard Column for P/N 19313 |
3µm |
Stainless Steel |
4.6 |
2 |
19306 |
TSKgel SuperHZ4000 |
3µm |
Stainless Steel |
6 |
15 |
19666 |
Guard Column for P/N 19306 |
3µm |
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperHZM-H
TSK-GEL SuperHZM-H mixed-bed columns are packed with rigid, porous, polymer beads each having a varying mean pore diameter. They are used for the non-aqueous separation of organic-soluble polymers with high molecular weights of less than 400,000,000 Da. The THF shipping solvent may be replaced by a limited number of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
10µm |
| Pore size (mean): |
mixed bed |
| Exclusion limit: |
400x10^6 Da |
| Max. temp. (°C): |
80 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- SuperHZ Series Ultra-High Throughput GPC Columns
|
TSK-GEL SuperHZ Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
19664 |
TSKgel SuperHZM-H |
10µm |
Stainless Steel |
4.6 |
15 |
19668 |
Guard Column for P/N 19664 |
3µm |
Stainless Steel |
4.6 |
2 |
19665 |
TSKgel SuperHZM-H |
10µm |
Stainless Steel |
6 |
15 |
19667 |
Guard Column for P/N 19665 |
10µm |
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperHZM-M
TSK-GEL SuperHZM-M mixed-bed columns are packed with rigid, porous, polymer beads each having a varying mean pore diameter. They are used for the non-aqueous separation of solvent-soluble high molecular weight polymers less than 4,000,000 Da. The THF shipping solvent may be replaced by a limited number of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
mixed bed |
| Exclusion limit: |
4x10^6 Da |
| Max. temp. (°C): |
80 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- SuperHZ Series Ultra-High Throughput GPC Columns
|
TSK-GEL SuperHZ Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
19662 |
TSKgel SuperHZM-M |
3µm |
Stainless Steel |
4.6 |
15 |
19314 |
Guard Column for P/N 19662 |
3µm |
Stainless Steel |
4.6 |
2 |
19663 |
TSKgel SuperHZM-M |
3µm |
Stainless Steel |
6 |
15 |
19666 |
Guard Column for P/N 19663 |
3µm |
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperHZM-N
TSKgel SuperHZM-N mixed-bed columns are packed with rigid, porous, polymer beads each having a varying mean pore diameter. They are used for the analysis of organic-soluble polymers with high molecular weights of less than 700,000 Da. The THF shipping solvent may be replaced by a limited number of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
mixed bed |
| Exclusion limit: |
700,000 Da |
| Max. temp. (°C): |
80 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Separation Report -- SuperHZ Series Ultra-High Throughput GPC Columns
|
TSK-GEL SuperHZ Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
19662 |
TSKgel SuperHZM-M |
3µm |
Stainless Steel |
4.6 |
15 |
19314 |
Guard Column for P/N 19662 |
3µm |
Stainless Steel |
4.6 |
2 |
19663 |
TSKgel SuperHZM-M |
3µm |
Stainless Steel |
6 |
15 |
19666 |
Guard Column for P/N 19663 |
3µm |
Stainless Steel |
4.6 |
3.5 |
|
|
TSK-GEL SuperMulitporeHZ-type Columns
The Tosoh GPC Tradition
Tosoh introduced its first line of GPC columns in 1971 and its first GPC instrument in 1972. Ever since, Tosoh scientists have made important contributions to advances in polymer analysis by Gel Permeation Chromatography.
At the 2008 Pittsburgh Conference, Tosoh Bioscience introduced the EcoSEC HLC-8320GPC GPC System, the 7th generation of dedicated GPC instruments. The EcoSEC GPC system allows faster MW determination at a lower cost per analysis. These advancements have been made possible by integrating HPLC system design with Tosoh's innovative multi-pore particle synthesis technology.
Multi-pore particle synthesis technology
Using this proprietary technology, Tosoh can manufacture particles containing a broad range of pore sizes. This innovative approach essentially creates a linear calibration curve within each particle. As a result, columns with an extended linear calibration curve can now be prepared without mixing particles of different pore sizes.
Products
First introduced in a 5 micron particle format, TSKgel MultiporeHxl-M column dimensions are 7.8mm ID x 30cm for use in conventional GPC systems.
Now, the innovative multi-pore technology is also available in 3, 4 and 6 micron TSK-GEL SuperMultiporeHZ-type columns, each featuring a distinctly different linear part of the calibration range as shown in the Figure on the right.
|
Calibration curves for TSK-GEL SuperMultiporeHZ columns with polystyrene standards
|
|
Multi-pore Technology
Graphical representations and technical data illustrate the multi-pore particle synthesis technology developed by Tosoh scientists.
The characteristics of the technology is shown in Figure 1. The scanning electron micrographs in Figuire 2 demonstrate the monodispersity of the particle size distribution, while Figure 3 shows that the pore volume of SuperMultiporeHZ particles is distributed over a wide range of pore sizes. The main benefit of multi-pore technology, a smooth chromatogram without inflection points, is shown in the chromatogram in Figure 4.
In general, columns with an extended linear clibration range can be constructed in one of three ways.
- The simplest way to obtain an extended linear calibration range is to couple columns, each containing particles with distinct pore size distributions. It is inevitable that the resulting calibration curve will deviate from linearity between each pore size range, resulting in irregularities in the chromatogram.
- The irregularities in chromatograms occur less frequently in the case of mixed-bed columns. Mixed-bed columns are prepared by mixing batches of particles each containing a narrow pore size distribution. However, inflection points in chromatograms can still arise due to a mismatch of pore sizes.
- Such inflection points are no longer observed with columns packed from particles prepared by multi-pore technology, as shown below in Figure 4.
Figure 1: Strategies to Expand Linear Calibration Curves in Size Exclusion Chromatography
|
|
Figure 2: SEM photograph of SuperMultiporeHZ-M particles prepared by multi-pore synthesis technology
|
Figure 3: Pore size distributions of particles in TSKgel SuperMultiporeHZ-M column and a mixed bed column
Figure 4: Comparison of TSKgel SuperMultiporeHZ-M and TSKgel SuperHZ for separation of Acrylic Resin
 |
|
TSKgel SuperMultiporeHZ-M,N,H
The innovative multi-pore technology is now also available in 3, 4 and 6 micron TSK-GEL SuperMultiporeHZ columns. Three columns, varying in linear range, are available. The base material of each TSKgel SuperMultiporeHZ column is poly(styrene/divinylbenzene).
As shown earlier, particles in TSK-GEL SuperMultiporeHZ columns are mono-disperse in size and exhibit a broad range of pore sizes. Each particle, by design, has an extended linear calibration curve, thereby greatly diminishing chromatograms with inflection points.
GPC chromatograms without inflection points . . . it will take some getting used to.
Product Attributes:
TSKgel |
SuperMultiporeHZ-N |
SuperMultiporeHZ-M |
SuperMultiporeHZ-H |
Base material: |
PS-DVB |
PS-DVB |
PS-DVB |
Particle size: |
3µm* |
4µm* |
6µm* |
Mean Pore size: |
80Å |
140Å |
ND |
Exclusion limit (PST/THF):
|
120,000 |
2,000,000 |
40,000,000 |
Separation range (Da): |
300~50,000 |
500~1,000,000 |
1,000~10,000,000 |
Theoretical plates/15cm column: |
20,000 |
16,000 |
11,000 |
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
21815 |
TSKgel SuperMultiporeHZ-N |
3μm |
Stainless Steel |
4.6 |
15 |
21816 |
TSKgel SuperMPHZ-N Guard |
|
Stainless Steel |
4.6 |
2 |
21488 |
TSKgel SuperMultiporeHZ-M |
4μm |
Stainless Steel |
4.6 |
15 |
21489 |
TSKgel SuperMPHZ-M Guard |
|
Stainless Steel |
4.6 |
2 |
21885 |
TSKgel SuperMultiporeHZ-H |
6μm |
Stainless Steel |
4.6 |
15 |
21886 |
TSKgel SuperMPHZ-H Guard |
|
Stainless Steel |
4.6 |
2 |
|
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