|
|
TSK-GEL GPC columns for organic-soluble polymers are packed with spherical particles composed of polystyrene crosslinked with divinylbenzene (PS-DVB). Main differences between the available product lines are highlighted in the Table below.
Tosoh redefines GPC calibration.
Tosoh's innovative multi-pore technology produces particles that contain a broad range of pore sizes. Columns with an extended linear calibration curve can now be prepared without mixing particles of different pore sizes.
TSK-GEL SuperH and TSK-GEL SuperHZ columns were developed for High-Throughput GPC applications. Both product lines feature particle sizes down to 3μm, a column length of 15cm and column diameters of 6mm ID and 4.6mm ID (SuperHZ only).
TSK-GEL Hxl and TSK-GEL Hhr columns are conventional GPC columns of 7.8mm ID x 30cm, while particles sizes are 5µm and larger for the larger pore size columns. |
Highlights
-
Tosoh manufactures two distinct product lines for GPC of organic-soluble polymers. While each line is based on particles prepared from polystyrene crosslinked with divinylbenzene, they differ in:
-
TSK-GEL Hxl-type, SuperHZ-type, and SuperMultiporeHZ-type columns are most inert to sample adsorption.
- TSK-GEL Hhr- and SuperH-type columns provide minimal shrinking and swelling of the column bed when changing solvents and allow operation up to 140°C.
- Small particle, narrow bore SuperMultiporeHZ, SuperHZ and SuperH columns provide high efficiency, short analysis times and operating cost.
- Except for SuperMultiporeHZ columns, all other TSK-GEL H-type column lines contain at least five different pore sizes.
- Each product line contains columns for the analysis of oligomers to polymers containing molecular weights as high as as 4 x 10^8Da (estimated).
-
All product lines contain a number of linear columns that are prepared by blending particles with different pore sizes.
|
Table: Distinguishing features of TSK-GEL H-type GPC column lines
Hxl and SuperHZ |
|
|
- Ultra-low adsorption columns with limited solvent range.
- Use SuperHZ for highest resolution and throughput.
- Use Hxl if you can live with longer analyis times.
- Max. temp: 60-80°C
|
- More linear than 'linear' mixed-bed columns.
- Improved MW accuracy and precision.
- Like SuperHZ and Hxl lines in terms of surface chemistry and temp. range.
- Like SuperHZ columns in terms of resolution and speed.
|
- Low adsorption columns with expanded solvent range.
- SuperH for high throughput and high resolution GPC.
- Use Hhr if you do not have a need to save time.
- Max. temp:140°C
|
|
TSK-GEL Hhr-type Columns for Expanded Solvent Compatibility
The TSK-GEL Hhr column line consists of eight columns with different pore sizes, TSKgel G1000Hhr through TSKgel G7000Hhr, and five columns with an extended linear range of the calibration curve. The calibration curves are shown below.
Calibration curves on TSK-GEL Hhr columns
|
|
Main characteristics
- Maximum operating temperature for Hhr-type columns is 140°C.
- Broad solvent range. All Hhr-type columns are shipped in THF, which can be exchanged for a wide variety of organic solvents as shown below.
- Hhr-type columns are conventional GPC columns with dimensions of 7.8mm ID x 30cm. Most HPLC systems do not require optimization of extra-column bandbroadening effects when working with conventional GPC columns.
Solvent compatibility for TSK-GEL Hhr (and SuperH) columns
The column shipping solvent limits the types of solvents that can be used with each column type.
Column type |
Shipping solvent* |
Can be replaced with |
SuperH and Hhr1 |
|
acetone, ethanol, quinoline, benzene, o-dichlorobenzene, ethyl acetate, dodecane, FC-113, carbon tetrachloride5, dichloromethane, dichloroethane, trichloroethane, n-hexane, cyclohexane, xylene, tetrahydrofuran, chloroform, 1,4-dioxane, hexafluoroisopropanol, toluene, 1-chloronaphthalene, dimethylaceto acetamide, methyl ethyl ketone, trichlorobenzene, m-cresol, dimethylformamide, methylpyrrolidone, o-chlorophenol/chloroform, dimethyl sulfoxide, pyridine
|
|
Notes:
1) In case of SuperH and Hhr, see table below for appropriate flow rates for solvent exchange. After switching to a very polar solvent, switching to a nonpolar is not recommended.
2) All TSK-GEL Hxl, Hhr, SuperHZ and SuperH analytical columns are shipped containing tetrahydrofuran (THF), except High Temperature (HT) columns, which contain o-dichlorobenzene.
3) Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.
* 100% methanol cannot be used with H-type columns; use this solvent with TSK-GEL SW-type or Alpha-type columns.
** TSK-GEL H columns may be specially ordered with this shipping solvent.
Recommended flow rates (mL/min) for solvent exchange in Super H and Hhr columns
Solvent |
SuperH (6.0mmIDx15cm) |
Hhr (7.8mmIDx30cm) |
n-Hexane |
0.50 |
0.9 |
methyl ethyl ketone |
0.40 |
0.7 |
dichloromethane, ethyl acetate |
0.35 |
0.6 |
toluene, chloroform |
0.30 |
0.5 |
dimethylformamide |
0.20 |
0.4 |
carbon tetrachloride, pyridine |
0.15 |
0.3 |
dimethyl sulfoxide, dioxane, ethanol, N-methylpyrrolidone, o-dichlorobenzene |
0.10 |
0.2 |
quinoline, hexafluoroisopropanol, 1-chloronaphthalene |
0.05 |
0.1 |
|
Recommended Solvents by Application for TSK-GEL H-type columns
Recommended solvent |
Application |
THF |
polystyrene, epoxy resin, phenoxy resin, polycarbonate, polyisoprene, polyvinyl acetate, polyvinyl chloride, monoglycerides, fatty acids, polybutadiene, poly(methyl methacrylate), poly(styrene-butadiene),
poly(styrene-acrylonitrile)
|
N,N-Dimethylformamide (DMF) +/- 5mM LiBr |
polyvinyl chloride, polyvinyl fluoride, urea resins, polyurethane, polystyrene, polyester, polyimido ether, polyimido ester, polyphenol (aqueous solution), polyacrylonitrile
|
o-Dichlorobenzene (ODCB) |
polyethylene, polypropylene
|
Chloroform |
polycarboxylic ether, acrylic resin, epoxy resin, polystyrene
|
m-Cresol/Chloroform |
nylon, polyseter, polyamide, poly (ethylene terephthalate)
|
Toluene |
polybutadiene, polysiloxane
|
|
Columns with linear calibration curves
The Hhr product line contains five linear, or mixed-bed columns, in which particles with different pore sizes are blended to provide an extended linear calibration curve. The mixe-bed columns feature increasing linear calibration ranges, from TSKgel GMHhr-L, GMHhr-N, GMHhr-M, to GMHhr-H. TSKgel GMHhr-H(S) HT is a high temeperature mixed bed column shipped in ODCB, o-dichlorobenzene.
TSKgel G1000Hhr
TSKgel G1000Hhr columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of small molecules and oligomers less than 1,000 Da. TSK-GEL G1000Hhr columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
15Å |
| Exclusion limit: |
< 1000 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel Hhr Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17352 |
TSKgel G1000Hhr |
5µm |
Stainless Steel |
7.8 |
30 |
17368 |
Guard Column for P/N 17352 |
|
Stainless Steel |
6 |
4 |
|
|
TSKgel G2000Hhr
|
TSKgel G2000Hhr columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of oligomers and polymers up to 10,000 Da molecular weight. TSK-GEL G2000Hhr columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
20Å |
| Exclusion limit: |
< 10,000 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel Hhr Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17353 |
TSKgel G2000Hhr |
5µm |
Stainless Steel |
7.8 |
30 |
17368 |
Guard Column for P/N 17353 |
|
Stainless Steel |
6 |
4 |
|
|
TSKgel G2500Hhr
TSKgel G2500Hhr columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of oligomers with molecular weights less than 20,000 Da. TSK-GEL G2500Hhr columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
30Å |
| Exclusion limit: |
20,000 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel Hhr Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17354 |
TSKgel G2500Hhr |
5µm |
Stainless Steel |
7.8 |
30 |
17368 |
Guard Column for P/N 17354 |
|
Stainless Steel |
6 |
4 |
|
|
TSKgel G3000Hhr
TSKgel G3000Hhr columns are packed with rigid, porous, polymer beads. They are from a class of columns used for the non-aqueous separation of oligomers and polymers less than 60,000 Da. TSK-GEL G3000Hhr columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
75Å |
| Exclusion limit: |
< 60,000 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel Hhr Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17355 |
TSKgel G3000Hhr |
5µm |
Stainless Steel |
7.8 |
30 |
17368 |
Guard Column for P/N 17355 |
5µm |
Stainless Steel |
6 |
4 |
|
|
TSKgel G4000Hhr
TSK-GEL G4000Hhr columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of polymers less than 400,000 Da. TSK-GEL G4000Hhr columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
200Å |
| Exclusion limit: |
< 400,000 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel Hhr Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17356 |
TSKgel G4000Hhr |
5µm |
Stainless Steel |
7.8 |
30 |
17368 |
Guard Column for P/N 17356 |
5µm |
Stainless Steel |
6 |
4 |
|
|
TSKgel G5000Hhr
TSKgel G5000Hhr columns are packed with rigid, porous, polymer beads. They are from a class of columns used for the non-aqueous separation of polymers less than 4,000,000 Da. TSK-GEL G4000Hhr columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
650Å |
| Exclusion limit: |
< 4x10^6 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel Hhr Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559s.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17357 |
TSKgel G5000Hhr |
5µm |
Stainless Steel |
7.8 |
30 |
17369 |
Guard Column for P/N 17357 |
5µm |
Stainless Steel |
6 |
4 |
|
|
TSKgel G6000Hhr
TSK-GEL G6000Hhr columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of polymers less than 40,000,000 Da. TSK-GEL G6000Hhr columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
ND |
| Exclusion limit (est.): |
< 4x10^7 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns,
Catalog -- Chromatography Catalog, SEC Columns, H-type
Conditions and Specifications
|
TSKgel Hhr Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17358 |
TSKgel G6000Hhr |
5µm |
Stainless Steel |
7.8 |
30 |
17369 |
Guard Column for P/N 17358 |
|
Stainless Steel |
6 |
4 |
|
|
TSKgel G7000Hhr
TSK-GEL G7000Hhr columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of polymers less than 400,000,000 Da.(estimate). TSK-GEL G7000Hhr columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
Matrix: |
polystyrene-
divinylbenzene |
Particle size (mean): |
5µm |
Pore size (mean): |
ND |
Exclusion limit (est.): |
< 4x10^8 Da |
Max. temp. (°C): |
140 |
Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel Hhr Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17359 |
TSKgel G7000Hhr |
5µm |
Stainless Steel |
7.8 |
30 |
17369 |
Guard Column for P/N 17359 |
|
Stainless Steel |
6 |
4 |
|
|
TSKgel GMHhr-H mixed bed
TSK-GEL GMHhr-H mixed bed columns are packed with rigid, multiple pore size, polymer beads. Mixed bed columns are commonly used for unknown samples and for the analysis of polymers with a broad molecular weight distribution. TSK-GEL GMHhr-H columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
TSK-GEL GMHhr-H columns are available in 5, 13, 20, and 30µm particle sizes. We recommend the use of TSK-GEL GMHhr-H (13, 20 or 30µm) to prevent or limit shearing of ultra high molecular weight polymers, if this occurs when using small (5µm) particle size columns.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5, 13, 20, and 30µm |
| Pore size (mean): |
mixed bed |
| Exclusion limit: |
ca. < 4x10^8 Da |
| Max. temp. (°C): |
80 |
| Shipping solvent: |
THF |
|
Literature:
Relevant Catalog Section 1
Relevant Catalog Section 2, in preparation
Operating Conditions and Specifications
|
|
TSK-GEL GMHhr Series Calibration Curves
TSK-GEL GMHhr-H Series Calibration Curves
Shear Degradation Comparison
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17360 |
TSKgel GMHhr-H |
5µm |
Stainless Steel |
7.8 |
30 |
17369 |
Guard column for P/N 17360 |
|
Stainless Steel |
6 |
4 |
17361 |
TSKgel GMHhr-H(S) |
13µm |
Stainless Steel |
7.8 |
30 |
17367 |
Guard column for P/N 17361 |
|
Stainless Steel |
7.5 |
7.5 |
18399 |
TSKgel GMHhr-H (20) |
20µm |
Stainless Steel |
7.8 |
30 |
18398 |
TSKgel GMHhr-H (30) |
30µm |
Stainless Steel |
7.8 |
30 |
18402 |
Guard column for P/Ns 18398, 18399 |
|
Stainless Steel |
7.5 |
7.5 |
|
|
TSKgel GMHhr-H(S) HT mixed bed
TSK-GEL GMHhr-H(S) HT mixed bed columns are packed with rigid, 13μm, polymer beads of various pore sizes. They are used for the high temperature analysis of very large polymers having a broad molecular weight range where shear forces need to be minimized. TSK-GEL GMHhr-H(S) HT columns are shipped in o-dichlorobenzene (ODCB). This solvent cannot be exchanged to other solvents.
The issue of shearing that occurs with the analysis of ultra high molecular weight polymers is overcome by the TSKgel GMHhr-H(S) HT column. The (S) is a reference to this shearing effect.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
13µm |
| Pore size (mean): |
mixed bed |
| Exclusion limit (est.): |
< 4x10^8 Da |
| Max. temp.(°C): |
80 |
| Shipping solvent: |
ODCB |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel Hhr Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
18393 |
TSKgel GMHhr-H(S) HT |
13µm |
Stainless Steel |
7.8 |
30 |
18397 |
Guard column for P/N 18393 |
30µm |
Stainless Steel |
6 |
4 |
|
|
TSKgel GMHhr-L mixed bed
TSKgel GMHhr-L mixed bed columns are packed with rigid, multiple pore size, polymer beads. Mixed bed columns are commonly used for unknown samples and for the analysis of polymers with a broad molecular weight distribution. TSK-GEL GMHhr-L columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
mixed bed |
| Exclusion limit (est.): |
4x10^6 Da |
| Max. temp. (°C): |
80 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel Hhr Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17362 |
TSKgel GMHhr-L |
5µm |
Stainless Steel |
7.8 |
30 |
| 17368 |
Guard Column for P/N 17362 |
|
Stainless Steel |
6 |
4 |
|
|
TSKgel GMHhr-M mixed bed
TSKgel GMHhr-M mixed bed columns are packed with rigid, multiple pore size, polymer beads. Mixed bed columns are commonly used for unknown samples and for the analysis of polymers with a broad molecular weight distribution. TSK-GEL GMHhr-M columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
TSK-GEL GMHhr-M columns are available in 5 and 13µm particle sizes. We recommend the use of TSK-GEL GMHhr-M (13µm) to prevent or limit shearing of ultra high molecular weight polymers, if this occurs when using small (5µm) particle size columns.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5 and 13µm |
| Pore size (mean): |
mixed bed |
| Exclusion limit: |
< 4x10^6 Da |
| Max. temp. (°C): |
80 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
Application Note -- Improved Reproducibility of MW Measurements of Polypeptoids using an EcoSEC GPC System
|
TSKgel Hhr Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17392 |
TSKgel GMHhr-M |
5µm |
Stainless Steel |
7.8 |
30 |
17369 |
Guard column for P/N 17392 |
5µm |
Stainless Steel |
6 |
4 |
17393 |
TSKgel GMHhr-M(S) |
13µm |
Stainless Steel |
7.8 |
30 |
17367 |
Guard column for P/N 17393 |
|
Stainless Steel |
7.5 |
7.5 |
|
|
TSKgel GMHhr-N mixed bed
TSKgel GMHhr-N mixed bed columns are packed with rigid, multiple pore size, polymer beads. Mixed bed columns are commonly used for unknown samples and for the analysis of polymers with a broad molecular weight distribution. TSK-GEL GMHhr-N columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
mixed bed |
| Exclusion limit: |
< 400,000 Da |
| Max. temp. (°C): |
80 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel Hhr Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
18055 |
TSKgel GMHhr-N |
5µm |
Stainless Steel |
7.8 |
30 |
17369 |
Guard Column for P/N 18055 |
|
Stainless Steel |
6 |
4 |
|
|
TSK-GEL SuperH-type Columns for Expanded Solvent Compatibility
The TSK-GEL SuperH column line consists of eight columns with different pore sizes, TSKgel SuperH1000 through TSKgel SuperH7000, and four columns with an extended linear range of the calibration curve. The calibration curves are shown below. SuperH-type columns are high efficiency/high throughput versions of the conventional Hhr columns. Both column types are based on the same bead chemistry.
Calibration curves on TSK-GEL SuperH columns
|
|
Main characteristics
- Maximum operating temperature for SuperH-type columns is 140°C.
- Broad solvent range. All SuperH-type columns are shipped in THF, which can be exchanged for a wide variety of organic solvents, as is shown below.
- SuperH-type columns are high efficiency, high-throughput gel permeation (HT-GPC) columns with dimensions of 6mm ID x 15cm.
Solvent compatibility for TSK-GEL SuperH and Hhr columns
The column shipping solvent limits the types of solvents that can be used with each column type.
Column type |
Shipping solvent* |
Can be replaced with |
SuperH and Hhr1 |
|
acetone, ethanol, quinoline, benzene, o-dichlorobenzene, ethyl acetate, dodecane, FC-113, carbon tetrachloride5, dichloromethane, dichloroethane, trichloroethane, n-hexane, cyclohexane, xylene, tetrahydrofuran, chloroform, 1,4-dioxane, hexafluoroisopropanol, toluene, 1-chloronaphthalene, dimethylaceto acetamide, methyl ethyl ketone, trichlorobenzene, m-cresol, dimethylformamide, methylpyrrolidone, o-chlorophenol/chloroform, dimethyl sulfoxide, pyridine
|
|
Notes:
1) In case of SuperH and Hhr, see table below for appropriate flow rates for solvent exchange. After switching to a very polar solvent, switching to a nonpolar is not recommended.
2) All TSK-GEL Hxl, Hhr, SuperHZ, and SuperH analytical columns are shipped containing tetrahydrofuran (THF), except High Temperature (HT) columns, which contain o-dichlorobenzene.
3) Prolonged exposure to carbon tetrachloride can corrode the stainless steel parts of a column and an HPLC system.
* 100% methanol cannot be used with H-type columns; use this solvent with TSK-GEL SW-type or Alpha-type columns.
** TSK-GEL H columns may be specially ordered with this shipping solvent.
Recommended flow rates (mL/min) for solvent exchange in SuperH and Hhr columns
Solvent |
SuperH (6.0mmIDx15cm) |
Hhr (7.8mmIDx30cm) |
n-Hexane |
0.50 |
0.9 |
methyl ethyl ketone |
0.40 |
0.7 |
dichloromethane, ethyl acetate |
0.35 |
0.6 |
toluene, chloroform |
0.30 |
0.5 |
dimethylformamide |
0.20 |
0.4 |
carbon tetrachloride, pyridine |
0.15 |
0.3 |
dimethyl sulfoxide, dioxane, ethanol,
N-methylpyrrolidone, o-dichlorobenzene |
0.10 |
0.2 |
quinoline, hexafluoroisopropanol,
1-chloronaphthalene |
0.05 |
0.1 |
|
Recommended Solvents by Application for TSK-GEL H-type columns
Recommended solvent |
Application |
THF |
polystyrene, epoxy resin, phenoxy resin, polycarbonate, polyisoprene, polyvinyl acetate, polyvinyl chloride, monglycerides, fatty acids, poly (butadiene), polybutadiene, poly (methyl methacrylate), poly (styrene-butadiene), poly (styrene-acrylonitrile)
|
N,N-Dimethylformamide (DMF) +/- 5mM LiBr |
polyvinyl chloride, polyvinyl fluoride, urea resins, polyurethane, polystyrene, polyester, polyimido ether, polyimido ester, polyphenol (aqueous solution), polyacrylonitrile
|
o-Dichlorobenzene (OCDB) |
polyethylene, polypropylene
|
Chloroform |
polycarboxylic ether, acrylic resin, epoxy resin, polystyrene
|
m-Cresol/Chloroform |
nylon, polyseter, polyamide, poly (ethylene terephthalate)
|
Toluene |
polybutadiene, polysiloxane
|
|
Beware of extra-column bandbroadening
The volume of a SuperH column is 3.4 times smaller than that of a conventional 7.8mm x 30cm column. As a result, peak volumes will be proportionally smaller on SuperH-type columns compared to a corresponding Hhr column. Thus, your HPLC system may require optimization of components that can give rise to extra-column bandbroadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant. Columns with linear calibration curves
The TSK-GEL SuperH product line contains four linear or mixed-bed columns, in which particles with different pore sizes are blended to provide an extended linear calibration curve. The mixed-bed columns feature increasing linear calibration ranges, from TSKgel SuperHM-L, SuperHM-N, SuperHM-M, to SuperHM-H.
TSKgel SuperH1000
TSK-GEL SuperH1000 columns are packed with rigid, porous, polymer beads. They are for the non-aqueous separation of small molecules and oligomers less than 1,000 Da. TSK-GEL SuperH1000 columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
15Å |
| Exclusion limit: |
1000 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel SuperH Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17990 |
TSKgel SuperH1000 |
3µm |
Stainless Steel |
6 |
15 |
18002 |
Guard Column for P/N 17990 |
|
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperH2000 columns are packed with rigid, porous, polymer beads. They are from a class of columns used for the non-aqueous separation of oligomers less than 10,000 Da.
TSK-GEL SuperH1000 columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
20Å |
| Exclusion limit: |
10,000 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel SuperH Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17991 |
TSKgel SuperH2000 |
3µm |
Stainless Steel |
6 |
15 |
18002 |
Guard Column for P/N 17991 |
|
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperH2500
TSK-GEL SuperH2500 columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of polymers with molecular weights of less than 20,000 Da.
TSK-GEL SuperH2500 columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
30Å |
| Exclusion limit: |
20,000 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel SuperH Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17992 |
TSKgel SuperH2500 |
3µm |
Stainless Steel |
6 |
15 |
18002 |
Guard Column for P/N 17992 |
|
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperH3000
TSKgel SuperH3000 columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of polymers with molecular weights less than 60,000 Da.
TSK-GEL SuperH columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
75Å |
| Exclusion limit: |
60,000 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel SuperH Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17993 |
TSKgel SuperH3000 |
3µm |
Stainless Steel |
6 |
15 |
18002 |
Guard Column for P/N 17993 |
|
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperH4000
TSK-GEL SuperH4000 columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of polymers less than 400,000 Da. TSK-GEL SuperH columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
200Å |
| Exclusion limit: |
400,000 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel SuperH Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17994 |
TSKgel SuperH4000 |
3µm |
Stainless Steel |
6 |
15 |
18002 |
Guard Column for P/N 17994 |
3µm |
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperH5000
TSK-GEL SuperH5000 columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of polymers less than 4,000,000 Da. TSK-GEL SuperH columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
650Å |
| Exclusion limit: |
4x10^6 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel SuperH Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559s.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17995 |
TSKgel SuperH5000 |
3µm |
Stainless Steel |
6 |
15 |
18003 |
Guard Column for P/N 17995 |
|
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperH6000
TSK-GEL SuperH6000 columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of polymers less than 40,000,000 Da (estimate). TSK-GEL SuperH columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
ND |
| Exclusion limit: |
40x10^6 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel SuperH Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559s.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17996 |
TSKgel SuperH6000 |
5µm |
Stainless Steel |
6 |
15 |
18003 |
Guard Column for P/N 17996 |
|
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperH7000
TSK-GEL SuperH7000 columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of polymers less than 400,000,000 Da (estimate). TSK-GEL SuperH columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
5µm |
| Pore size (mean): |
ND |
| Exclusion limit (est.): |
400x10^6 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications
|
TSKgel SuperH Calibration Curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17997 |
TSKgel SuperH7000 |
5µm |
Stainless Steel |
6 |
15 |
18003 |
Guard Column for P/N 17997 |
|
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperHM-H
TSK-GEL SuperHM-H mixed-bed columns are packed with rigid polymer beads with varying pore sizes. They are used for the non-aqueous separation of polymers with a broad molecular weight range or for analyzing unknown samples. TSK-GEL SuperH columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
mixed |
| Exclusion limit (est.): |
400x10^6 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications: In Preparation
|
TSKgel SuperH mixed bed calibration curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
18001 |
TSKgel SuperHM-H |
3µm |
Stainless Steel |
6 |
15 |
18003 |
Guard Column for P/N 18001 |
3µm |
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperHM-L
TSKgel SuperHM-L mixed-bed columns are packed with rigid polymer beads with varying pore sizes. They are used for the non-aqueous separation of polymers with a broad molecular weight range or for analyzing unknown samples. TSK-GEL SuperH columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
mixed |
| Exclusion limit: |
4x10^6 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications: In Preparation
|
TSKgel SuperH mixed bed calibration curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17998 |
TSKgel SuperHM-L |
3µm |
Stainless Steel |
6 |
15 |
18003 |
Guard Column for P/N 17998 |
|
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperHM-M
TSK-GEL SuperHM-M columns are packed with rigid, various pore size, polymer beads. They are used for the non-aqueous separation of polymers with a broad molecular weight range or for analyzing unknown samples. TSK-GEL SuperH columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
mixed |
| Exclusion limit: |
4x10^6 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications: In Preparation
|
TSKgel SuperH mixed bed calibration curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
18000 |
TSKgel SuperHM-M |
3µm |
Stainless Steel |
6 |
15 |
18003 |
Guard Column for P/N18000 |
|
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperHM-N
| TSKgel SuperHM-N mixed-bed columns are packed with rigid, various pore size, polymer beads. They are used for the non-aqueous separation of polymers with a broad molecular weight range or for analyzing unknown samples. TSK-GEL SuperH columns are shipped in THF, which can be exchanged for a wide variety of organic solvents.
|
Product Attributes:
| Matrix: |
polystyrene-divinylbenzene |
| Particle size (mean): |
3µm |
| Pore size (mean): |
mixed |
| Exclusion limit (est.): |
4x10^5 Da |
| Max. temp. (°C): |
140 |
| Shipping solvent: |
THF |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, H-type
Operating Conditions and Specifications: In Preparation
|
TSKgel SuperH mixed bed calibration curves |
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
17999 |
TSKgel SuperHM-N |
3µm |
Stainless Steel |
6 |
15 |
18003 |
Guard column for P/N 17999 |
|
Stainless Steel |
4.6 |
3.5 |
|
|
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