|
|
TSK-GEL SW-type columns are silica-based. A hydrophilic diol-type bonded phase shields the silica surface from interacting with protein samples. Three different pore sizes are available.
- 125Å pore size for analysis of small proteins and peptides
- 250Å pore size for most protein samples
- 450Å pore size for very large proteins and nucleic acids
Compared to competitive HPLC columns, SW-type columns contain more pore volume per unit column volume, which results in higher MW selectivity or better resolution.
Particle sizes for the three SW-type column lines are:
- SuperSW: 4 micron
- SWxl: 5-8 micron
- SW: 10-13 micron
See each of the SW-type product lines for representative calibration curves.
|
Highlights
- TSK-GEL SW-type packings are comprised of rigid spherical silica gel particles chemically derivatized with diol-containing ligands.
- Low adsorption and well-defined pore size distribution, which are requirements for high performance Gel Filtration of proteins.
- Three pore sizes are available in SW or SWxl product lines, two in the SuperSW product line
- TSK-GEL SuperSW columns feature 4 micron particles for the highest resolution. The narrow 4.6mm column ID provides the highest sensitivity when limited in sample mass.
- Semi-preparative (21.5mm ID) and preparative (55mm, 108mm ID) stainless steel columns are available for precise scale-up to commercial production.
- PEEK and Glass column hardware are available in the SWxl product line.
|
|
TSK-GE
SWxl |
TSK-GEL
SW |
TSK-GEL SuperSW columns contain spherical 4µm particles (125Å and 250Å only) packed in 30cm x 4.6mm ID columns for:
|
Use 5 or 8µm particle size TSK-GEL SWxl columns to improve sample resolution or to reduce analysis time (over SW columns).
|
Analytical TSK-GEL SW column line features 10 or 13µm particle sizes for improved sample capacity.
|
|
TSK-GEL SuperSW
TSK-GEL SuperSW columns, introduced in 1997, represent the third generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.
Particle Characteristics
TSK-GEL SuperSW-type columns contain smaller particles than SWxl-type columns; 4 micron versus 5 micron. In addition, the column internal diameter has been reduced from 7.8mm ID to 4.6mm ID to provide higher sensitivity in sample-limited cases and to cut down on solvent use.
HPLC System Requirements
It is important to employ an HPLC system that is optimized with regards to extra-column bandbroadening to take full advantage of the high column efficiency that can be obtained on SuperSW columns. See Separation Report 95 for recommendations.
Calibration Curves
The figure to the right shows the calibration curves for globular proteins for the two TSK-GEL SuperSW-type columns in comparison with those for the corresponding SWxl columns. The following table summarizes the molecular weight ranges for SuperSW2000 and SuperSW3000 columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the pores. For comparative purposes, similar data is provided as well for TSK-GEL SW and SWxl-type columns. |
Protein Calibration curves for
TSK-GEL SuperSW and SWxl
|
|
Properties and separation ranges for TSK-GEL SW-type packings (Molecular Weight in Da)
TSK-GEL packing |
Particle
size (µm) |
Pore
size (Å) |
Globular
proteins |
Dextrans |
Polyethylene
glycols and oxides |
SuperSW2000 |
4 |
125 |
5,000 - 1.5 x 10^5 |
|
|
G2000SWxl
BioAssist G2SWxl |
5 |
125 |
5,000 - 1.5 x 10^5 |
|
|
QC-PAK TSK200 |
5 |
125 |
5,000 - 1.5 x 10^5 |
|
|
G2000SW |
10, 13 |
125 |
5,000 - 1 x 10^5 |
|
|
|
|
|
|
|
|
SuperSW3000 |
4 |
250 |
1 x 10^4 - 5 x 10^5 |
|
|
G3000SWxl
BioAssist G3SWxl |
5 |
250 |
1 x 10^4 - 5 x 10^5 |
|
|
QC-PAK TSK300 |
5 |
250 |
1 x 10^4 - 5 x 10^5 |
|
|
G3000SW |
10 |
250 |
1 x 10^4 - 5 x 10^5 |
|
|
| |
|
|
|
|
|
G4000SWxl
BioAssist G4SWxl |
8 |
450 |
2 x 10^4 - 7 x 10^6 |
|
|
G4000SW |
13, 17 |
450 |
2 x 10^4 - 7 x 10^6 |
|
|
|
Data in the Table were generated using the following conditions:
Columns: |
Two 4µm, 4.6mmIDx30cm TSK-GEL SuperSW columns in series; two 5µm, 7.8mmIDx30cm TSK-GEL SWxl columns in series; two 10µm, 7.5mmIDx60cm TSK-GEL SW columns in series |
Elution: |
Globular proteins: 0.3M NaCl in 0.1M (0.05M for SWxl columns) phosphate buffer, pH 7
Dextrans and polyethylene glycols and oxides (PEOs): distilled water |
TSKgel SuperSW2000
Featuring ultra-efficient 4μm particles, TSKgel SuperSW2000 provides higher efficiency than 5μm TSKgel G2000SWxl columns. The 125Å pore size results in a fractionation range up to 150,000 Da for globular proteins. The narrow 4.6mm column inner diameter reduces buffer consumption and is ideal for sample-limited applications.
To fully benefit from the high efficiency of 4μm columns, and because the column diameter of TSK-GEL SuperSW columns is 4.6mm, it is important to minimize the amount of dead volume in the HPLC system. Consult the Separation Report for details (provided below).
|
Product Attributes:
| Base material: |
Silica |
| Particle size (mean): |
4µm |
| Pore size (mean): |
125Å |
| Functional group: |
Diol |
| pH stability: |
2.5 - 7.5 |
| Calibration range: |
5,000-150,000 Da (globular proteins) |
|
Literature:
Application Overview -- Gel Filtration Chromatography with TSK-GEL columns
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, SW-type
Operating Conditions and Specifications
Product Overview -- TSK-GEL SuperSW2000 and SuperSW3000 columns
Separation Report --TSK-GEL SuperSW Series (rev. 10/07)
|
Applications |
Separation of Mixture of Protein/peptide
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
| 18674 |
TSKgel SuperSW2000 |
4µm |
Stainless Steel |
4.6 |
30 |
| 18762 |
Guard Column for P/N 18674 |
|
Stainless Steel |
4.6 |
3.5 |
|
|
TSKgel SuperSW3000
Featuring ultra-efficient 4μm particles, TSKgel SuperSW3000 provides higher efficiency than 5μm TSKgel G3000SWxl columns. The 250Å pore size results in a fractionation range up to 500,000 Da for globular proteins. The narrow 4.6mm column inner diameter reduces buffer consumption and is ideal for sample-limited applications.
To fully benefit from the high efficiency of 4μm columns, and because the column diameter of TSK-GEL SuperSW columns is 4.6mm or less, it is important to minimize the amount of dead volume in the HPLC system. Consult the Separation Report for details (provided below).
|
Product Attributes:
| Base material: |
Silica |
| Particle size (mean): |
4µm |
| Pore size (mean): |
250Å |
| Functional group: |
Diol |
| pH stability: |
2.5 - 7.5 |
| Calibration range: |
10,000-500,000 Da(globular proteins) |
|
Literature:
Application Note -- Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column
Application Note -- The Characterization of Biological Samples by Microbore TSK-GEL SuperSW3000 Size Exclusion Columns
Application Note -- Stability of TSK-GEL SuperSW3000 Columns for Analyzing Proteins by SEC
Application Overview -- Gel Filtration Chromatography with TSK-GEL columns
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, SW-type
Operating Conditions and Specifications
Product Overview -- TSK-GEL SuperSW2000 and SuperSW3000 columns
Separation Report --TSK-GEL SuperSW Series (rev. 10/07)
Technical Presentation -- High sensitivity protein separations using a new size exclusion chromatography microcolumn for use in conjunction with MS
|
Applications |
Overlays of monoclonal antibody separation with 0.2M NaCl, 0.2M NaClO4 and 0.4M NaClO4
(Enlargement of fusion protein 1's baseline region shows improved peak shape of the dimer and trimer aggregates with 0.4M NaClO4)
Published with permission of Scott Lauder, Lexigen Pharmaceuticals
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
18675 |
TSKgel SuperSW3000 |
4µm |
Stainless Steel |
4.6 |
30 |
18762 |
Guard Column for P/N 18765 |
|
Stainless Steel |
4.6 |
3.5 |
21485 |
TSKgel SuperSW3000 |
|
Stainless Steel |
2 |
30 |
21845 |
TSKgel SuperSW3000 |
4µm |
Stainless Steel |
1 |
30 |
|
|
TSK-GEL SWxl columns
TSK-GEL SWxl columns, introduced in 1987, represent the second generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.
Particle Characteristics
TSK-GEL SWxl-type columns contain smaller particles than SW-type columns; 5 and 8μm versus 10 and 13μm. As the SW-type columns, TSK-GEL SWxl columns feature highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSK-GEL SWxl-type columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes. These columns are commonly used in the quality control of monoclonal antibodies and other biopharma products.
Calibration Curves
The Figure below shows the calibration curves for globular proteins, polyethylene oxides and dextrans for each of the three TSK-GEL SWxl-type columns. The following Table summarizes the molecular weight ranges for G2000SWxl, G3000SWxl and G4000SWxl columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the pores. For comparative purposes, similar data is provided as well for TSK-GEL SW and SuperSW-type columns. |
Protein Calibration curves for
TSK-GEL SWxl columns
|
|
Properties and separation ranges for TSK-GEL SW-type packings (Molecular Weight in Da)
TSK-GEL packing |
Particle
size (µm) |
Pore
size (Å) |
Globular
proteins |
Dextrans |
Polyethylene
glycols and oxides |
SuperSW2000 |
4 |
125 |
5,000 - 1.5 x 10^5 |
|
|
G2000SWxl
BioAssist G2SWxl |
5 |
125 |
5,000 - 1.5 x 10^5 |
|
|
QC-PAK TSK200 |
5 |
125 |
5,000 - 1.5 x 10^5 |
|
|
G2000SW |
10, 13 |
125 |
5,000 - 1 x 10^5 |
|
|
|
|
|
|
|
|
SuperSW3000 |
4 |
250 |
1 x 10^4 - 5 x 10^5 |
|
|
G3000SWxl
BioAssist G3SWxl |
5 |
250 |
1 x 10^4 - 5 x 10^5 |
|
|
QC-PAK TSK300 |
5 |
250 |
1 x 10^4 - 5 x 10^5 |
|
|
G3000SW |
10 |
250 |
1 x 10^4 - 5 x 10^5 |
|
|
| |
|
|
|
|
|
G4000SWxl
BioAssist G4SWxl |
8 |
450 |
2 x 10^4 - 7 x 10^6 |
|
|
G4000SW |
13, 17 |
450 |
2 x 10^4 - 7 x 10^6 |
|
|
|
Data generated using the following conditions:
Columns: |
Two 4µm, 4.6mmIDx30cm TSK-GEL SuperSW columns in series; two 5µm, 7.8mmIDx30cm TSK-GEL SWxl columns in series; two 10µm, 7.5mmIDx60cm TSK-GEL SW columns in series |
Elution: |
Globular proteins: 0.3M NaCl in 0.1M (0.05M for SWxl columns) phosphate buffer, pH 7
Dextrans and polyethylene glycols and oxides (PEOs): distilled water |
TSK-GEL G2000SWxl
Widely considered the premium columns for analyzing biopolymers by GFC, TSK-GEL G2000SWxl columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.
With 5μm particles and 125Å pores, TSKgel G2000SWxl is an excellent choice for small proteins and peptide separations. The fractionation range is applicable for globular protein samples up to 150,000 Da.
|
Product Attributes:
| Base material: |
Silica |
| Particle size (mean): |
5µm |
| Pore size (mean): |
125Å |
| Functional group: |
Diol |
| pH stability: |
2.5-7.5 |
| Calibration range: |
5,000 - 150,000 (globular proteins) |
|
Literature:
Application Overview -- Purification of Nucleic Acids
Application Overview -- Gel Filtration Chromatography with TSK-GEL columns
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, SW-type
Operating Conditions and Specifications
Separation Report -- Separation of Monoclonal Antibodies Using TSK-GEL HPLC Columns
|
Applications |
Higher resolution with TSKgel G2000SWxl
compared with TSKgel G2000SW
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
|
QC-PAK GFC 200GL |
|
Glass |
7.8 |
15 |
|
BioAssist G2SWxl |
|
PEEK |
7.8 |
30 |
|
Guard Column for P/N 20027 |
|
PEEK |
8 |
4 |
|
QC-PAK GFC 200 |
|
Stainless Steel |
7.8 |
15 |
|
TSKgel G2000SWxl |
|
Stainless Steel |
7.8 |
30 |
|
Guard Column for P/N 08540, 16215 |
|
Stainless Steel |
6 |
4 |
|
TSKgel Top-Off for SWxl and QC-PAK, 1g |
|
|
|
|
|
|
TSK-GEL G3000SWxl
Widely considered the premium columns for analyzing biopolymers by GFC, TSK-GEL G3000SWxl columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.
With 5μm particles and 250Å pores, TSKgel G3000SWxl is an excellent choice for protein and large peptide separations. The fractionation range is applicable for globular protein samples up to 0.5 million Da.
|
Product Attributes:
| Base material: |
Silica |
| Particle size (mean): |
5μm |
| Pore size (mean): |
250Å |
| Functional group: |
Diol |
| pH stability: |
2.5-7.5 |
| Calibration range: |
1,000-500,000 Da (globular proteins) |
|
Literature:
Application Note -- Improve Recovery & Reproducibility with Pre-Treatment of Analytical TSK-GEL G3000SWxl Size Exclusion Columns
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, SW-type
Operating Conditions and Specifications
Separation Report -- Separation of IgG and Albumin by High Performance Gel Filtration Chromatography Using TSK-GEL G3000SWxl
Application Overview -- Gel Filtration Chromatography with TSK-GEL columns
Application Overview -- Purification of Nucleic Acids
Separation Report -- Separation of Monoclonal Antibodies Using TSK-GEL HPLC Columns
|
Applications |
Separation of crude protein samples on TSKgel G3000SWxl
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
|
QC-PAK GFC 300GL |
|
Glass |
8 |
15 |
| 20026 |
TSKgel BioAssist G3SWxl |
|
PEEK |
7.8 |
30 |
18008 |
Guard Column for P/N 20026 |
|
PEEK |
6 |
4 |
|
QC-PAK GFC 300 |
|
Stainless Steel |
7.8 |
15 |
|
TSKgel G3000SWxl |
|
Stainless Steel |
7.8 |
30 |
|
Guard Column for P/N 08541 |
|
Stainless Steel |
6 |
4 |
|
TSKgel SWxl Top-Off for SWxl/QC-PAK, 1g |
|
|
|
|
|
|
TSK-GEL G4000SWxl
Widely considered the premium columns for analyzing biopolymers by GFC, TSK-GEL G4000SWxl columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.
With 8μm particles and 450Å pores, TSKgel G4000SWxl provides the largest exclusion limit and the widest fractionation range. It is an excellent choice for pegylated proteins or glycosylated biomolecules. The fractionation range is applicable for globular protein samples up to 7 million Da.
|
Product Attributes:
| Base material: |
Silica |
| Particle size (mean): |
8µm |
| Pore size (mean): |
450Å |
| Functional group: |
Diol |
| pH stability: |
2.5 - 7.5 |
| Calibration range: |
1,000-7x10^6Da (globular proteins) |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, SW-type
Operating Conditions and Specifications
Application Overview -- Gel Filtration Chromatography with TSK-GEL columns
Application Overview -- Purification of Nucleic Acids
Separation Report -- Separation of Monoclonal Antibodies Using TSK-GEL HPLC Columns
|
Applications |
Higher resolution with TSKgel G4000SWxl
compared with TSKgel G4000SW
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
|
TSKgel BioAssist G4SWxl |
|
PEEK |
7.8 |
30 |
|
Guard Column for P/N 20025 |
|
PEEK |
6 |
4 |
|
TSKgel G4000SWxl |
|
Stainless Steel |
7.8 |
30 |
|
Guard Column for P/N 08542 |
|
Stainless Steel |
6 |
4 |
08544 |
TSKgel Top-Off for SWxl, 1g |
5μm |
|
|
|
|
|
TSK-GEL SW columns
TSK-GEL SW columns, introduced in 1977, were the first of a long line of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.
Particle Characteristics
SW-type columns are based on highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSK-GEL SW-type columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes.
Calibration Curves
The figure below shows the calibration curves for globular proteins, polyethylene oxides and dextrans for each of the three TSK-GEL SW-type columns. The following table summarizes the molecular weight ranges for G2000SW, G3000SW and G4000SW columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the particle pores. For comparative purposes, similar data is provided as well for TSK-GEL SWxl and SuperSW-type columns.
Polyethylene oxide, dextran and protein calibration curves for TSK-GEL SW columns
Properties and separation ranges for TSK-GEL SW-type packings (Molecular Weight in Da)
TSK-GEL packing |
Particle
size (µm) |
Pore
size (Å) |
Globular
proteins |
Dextrans |
Polyethylene
glycols and oxides |
SuperSW2000 |
4 |
125 |
5,000 - 1.5 x 10^5 |
|
|
G2000SWxl/
BioAssist G2SWxl |
5 |
125 |
5,000 - 1.5 x 10^5 |
|
|
QC-PAK TSK200 |
5 |
125 |
5,000 - 1.5 x 10^5 |
|
|
G2000SW |
10, 13 |
125 |
5,000 - 1 x 10^5 |
|
|
|
|
|
|
|
|
SuperSW3000 |
4 |
250 |
1 x 10^4 - 5 x 10^5 |
|
|
G3000SWxl/
BioAssist G3SWxl |
5 |
250 |
1 x 10^4 - 5 x 10^5 |
|
|
QC-PAK TSK300 |
5 |
250 |
1 x 10^4 - 5 x 10^5 |
|
|
G3000SW |
10 |
250 |
1 x 10^4 - 5 x 10^5 |
|
|
| |
|
|
|
|
|
G4000SWxl/
BioAssist G4SWxl |
8 |
450 |
2 x 10^4 - 7 x 10^6 |
|
|
G4000SW |
13, 17 |
450 |
2 x 10^4 - 7 x 10^6 |
|
|
|
Data generated using the following conditions:
Columns: |
Two 4µm, 4.6mmIDx30cm TSK-GEL SuperSW columns in series; two 5µm, 7.8mmIDx30cm TSK-GEL SWxl columns in series; two 10µm, 7.5mmIDx60cm TSK-GEL SW columns in series |
Elution: |
Globular proteins: 0.3M NaCl in 0.1M (0.05M for SWxl columns) phosphate buffer, pH 7
Dextrans and polyethylene glycols and oxides (PEOs): distilled water |
TSKgel G2000SW
As the first generation of silica-based SEC columns, TSKgel G2000SW provides excellent separation of peptides and proteins with molecular weights up to 100,000 Da.
TSK-GEL G2000SW columns start at 10μm for analytical columns and also scale to 13 and 20μm in columns up to 55mm ID for preparative purifications.
|
Product Attributes:
Base material: |
Silica |
Particle size (mean): |
10μm and 13μm |
Pore size (mean): |
125Å |
Functional group: |
Diol |
pH stability: |
2.5 - 7.5 |
Calibration range: |
5,000-1x10^5Da (globular proteins) |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, SW-type
Operating Conditions and Specifications
Application Overview -- Gel Filtration Chromatography with TSK-GEL columns
Application Overview -- Purification of Nucleic Acids |
Applications |
Comparison of the pore sizes of TSK-GEL SW packings for the separation of a protein mixture
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
05788 |
TSKgel G2000SW |
10μm |
Stainless Steel |
7.5 |
30 |
05102 |
TSKgel G2000SW |
10μm |
Stainless Steel |
7.5 |
60 |
05371 |
Guard column for P/N 05788 and 05102 |
|
Stainless Steel |
7.5 |
7.5 |
06727 |
TSKgel G2000SW |
|
Stainless Steel |
21.5 |
30 |
|
TSKgel G2000SW |
|
Stainless Steel |
21.5 |
60 |
|
Guard Column for P/N 06727 and 05146 |
|
Stainless Steel |
21.5 |
7.5 |
|
TSKgel SW Top-Off for 7.5mm ID SW, 1g |
10μm |
|
|
|
|
|
TSK-GEL G3000SW
As the first generation of silica-based GFC columns, TSK-GEL G3000SW columns provide excellent separation of proteins and other biomolecules with molecular weights up to 500,000 Da.
The TSKgel G3000SW columns start at 10μm for analytical columns and also scale to 13 and 20μm in columns up to 55mm ID for preparative purifications.
|
Product Attributes:
| Base material: |
Silica |
| Particle size (mean): |
10μm and 13μm |
| Pore size (mean): |
250Å |
| Functional group: |
Diol |
| pH stability: |
2.5 -7.5 |
| Calibration range: |
2,000-5x10^5Da (globular proteins) |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, SW-type
Operating Conditions and Specifications
Application Overview -- Gel Filtration Chromatography with TSK-GEL columns
Application Overview -- Purification of Nucleic Acids
Separation Report -- Separation of Monoclonal Antibodies Using TSK-GEL HPLC Columns |
Applications |
Comparison of the pore sizes of TSK-GEL SW packings for the separation of a protein mixture
|
|
|
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
08800 |
TSKgel G3000SW |
10μm |
Glass |
8 |
30 |
|
Guard Column for P/N 08800 |
|
Glass |
8 |
4 |
05789 |
TSKgel G3000SW |
10μm |
Stainless Steel |
7.5 |
30 |
05103 |
TSKgel G3000SW |
10μm |
Stainless Steel |
7.5 |
60 |
05371 |
Guard Column for P/Ns 05789, 05103 |
|
Stainless Steel |
7.5 |
7.5 |
|
TSKgel Top-Off for 7.5mmID SW, 1 g |
|
Stainless Steel |
|
|
|
TSKgel G3000SW |
|
Stainless Steel |
21.5 |
30 |
|
TSKgel G3000SW |
|
Stainless Steel |
21.5 |
60 |
|
Guard Column for P/Ns 06728, 05147 |
|
Stainless Steel |
21.5 |
7.5 |
|
|
TSK-GEL G4000SW
As the first generation of silicia based SEC columns, the TSK-GEL G4000SW columns provide excellent separation of proteins and other biomolecules.
TSK-GEL G4000SW columns are available in 13μm particle size in Glass and Stainless Steel analytical columns. Particle sizes of 13 and 17μm are used to pack 30cm and 60cm x 21.5mm ID columns, respectively, for semi-preparative purifications.
|
Product Attributes:
| Base material: |
Silica |
| Particle size (mean): |
13μm and 17μm |
| Pore size (mean): |
450Å |
| Functional group: |
Diol |
| pH stability: |
2.5 -7.5 |
| Calibration range: |
2,000-7x10^6 Da (globular proteins) |
|
Literature:
Catalog -- Chromatography Catalog, SEC Columns, Overview
Catalog -- Chromatography Catalog, SEC Columns, SW-type
Operating Conditions and Specifications
Application Overview -- Gel Filtration Chromatography with TSK-GEL columns
Application Overview -- Purification of Nucleic Acids
Separation Report -- Separation of Monoclonal Antibodies Using TSK-GEL HPLC Columns |
Applications |
Comparison of the pore sizes of TSK-GEL SW packings for the separation of a protein mixture
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
| 08801 |
TSKgel G4000SW |
|
Glass |
8 |
30 |
| 08805 |
Guard column for P/N 08801 |
|
Glass |
8 |
4 |
|
TSKgel G4000SW |
|
Stainless Steel |
7.5 |
30 |
|
TSKgel G4000SW |
|
Stainless Steel |
7.5 |
60 |
05371 |
Guard column for P/N 05790 and 05104 |
|
Stainless Steel |
7.5 |
7.5 |
|
TSKgel SW Top-Off, 1g for P/N 5790, 05104 |
|
|
|
|
|
TSKgel G40000SW |
|
Stainless Steel |
21.5 |
30 |
|
TSKgel G4000SW |
17μm |
Stainless Steel |
21.5 |
60 |
|
Guard column for P/N 06729, 05148 |
|
Stainless Steel |
21.5 |
7.5 |
|
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For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.
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