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TSK-GEL kolonner
TSK-GEL Columns for Analyzing Water-soluble Polymers
Size Exclusion: Gel Filtration Chromatography
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MD Scientific is authorized distributor in Denmark for Tosoh Biobioscience
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(covers all TSKgel columns for SEC with aqueous mobile phases, all aspects of size exclusion chromatography (SEC): Fundamentals of SEC, features of TSKgel SEC columns, and application examples for the different column types:
- Silica based TSKgel SW columns for protein analysis
- Polymer basedcolumns for water soluble synthetic polymers and polysaccharides
- TSKgel Alpha andSuperAW columns suitable for aqueous and organic mobile phase
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TSK-GEL PWxl- and PW-type columns are the columns of choice for analyses of water-soluble polymers. All TSK-GEL polymer-based gel filtration columns are prepared from polymethacrylate resin.
PWxl- and PW-type
- Use higher efficiency PWxl-type columns for optimal resolution, to reduce analysis time, or in sample limited applications.
- Use PW-type columns when analysis time is not critical, when sample mass is not limited, to collect fractions, or to obtain maximum number of plates (at the expense of analysis time).
PWxl-CP columns
- PWxl-CP columns have been chemically modified to improve recovery of cationic polymers.
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Highlights:
- PW, PWxl and PWxl-CP columns
- Hydrophilic methacrylate beads.
- Particle sizes are 7-13μm for PWxl and PWxl-CP columns, and 12-17μm for PW-type.
- Higher % crosslinking for smaller particle size PWxl-type resins.
- Stable from pH 2 to 12
- Eluent: water or buffer (up to 20% methanol/80% aqueous)
- G6000PW is available in PEEK column hardware for ultra-low sample adsorption during virus analysis.
- Temperatures up to 80˚C (50˚C for TSKgel G-DNA-PW).
- PWxl-CP columns
- Minimal adsorption of cationic polymers.
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TSK-GEL PW and PWxl |
TSK-GEL PWxl-CP |
As TSK-GEL PW-type columns, there are seven TSK-GEL PWxl-type columns varying in pore size from less than 100Å to more than 1000Å. Mixed-bed columns are also available.
The TSK-GEL PWxl product line also offers specialty columns for analyzing:
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Carbohydrate oligomers
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DNA and RNA fragments
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TSK-GEL PWxl-CP columns have the same base resin as the PWxl columns and were specifically developed for the analysis of water-soluble cationic polymers and allow elution under low salt conditions.
Avaliable PWxl-CP-type columns and pore sizes:
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G3000PWxl-CP [200Å]
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G5000PWxl-CP [1000Å]
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G6000PWxl-CP [>1000Å]
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TSK-GEL PW-type Columns for Analysis of Water-soluble Polymers
TSK-GEL PW columns are composed of spherical, hydrophilic polymethacrylate beads. Particle sizes range from 12µm for the smaller pore size columns to 17µm for the larger pore size columns.
The main application areas for PW columns are the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides.
The figure to the right shows the calibration curves for all PW-type columns. Suitable solvents for various polymer samples are shown in the table below.
Specialty PW-type Columns
The mixed-mode column, TSKgel GMPW, has an extended linear calibration range, suitable for samples with a broad MW distribution, as well as unknown samples.
TSKgel G2000PW, the larger particle size equivalent of TSKgel G-Oligo-PW, is most suitable for semipreparative and preparative isolation of oligosaccharides.
TSKgel G1000PW is the preferred column for analysis of low MW oligomers. |
Polyethylene glycol and oxide calibration curves for TSK-GEL PW columns
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Recommended eluents for GFC of water-soluble polymers on TSK-GEL PW-type columns
Type of polymer |
Typical sample |
Suitable eluent |
Nonionic hydrophilic |
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soluble starch, methyl cellulose, pullulan
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dextran, hydroxyethyl cellulose
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polyvinyl alcohol, polyacrylamide
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Buffer or salt solution (e.g. 0.1-0.5M NaNo3)
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Nonionic hydrophobic |
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Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1M NaNO3)
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Anionic hydrophilic |
sodium chondroitin sulfate, sodium alginate, carboymethyl cellulose, sodium polyacrylate, sodium hyaluronate
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Buffer or salt solution (e.g. 0.1M NaNO3)
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sulfonated lignin sodium salt, sodium polystyrenesulfonate
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Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1M NaNO3)
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Cationic hydrophilic |
glycol chitosan, DEAE-dextran, poly(ethyleneimine), poly(trimethylaminoethyl methacrylate) iodide salt
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0.5M acetic acid with 0.3M Na2SO4 or 0.8M NaNO3
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Cationic hydrophobic |
poly(4-vinylbenzyltrimethylammonium chloride), poly(N-methyl-2-vinylpyridinium) iodide salt
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0.5M acetic acid with 0.3M Na2SO4
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Amphoteric hydrophilic |
peptides, proteins, poly- and oligosaccharides, DNA, RNA
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Buffer or salt solution (e.g. 0.1M NaNO3)
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Amphoteric hydrophobic |
blue dextran, collagen, gelatin, hydrophobic proteins, hydrophobic peptides
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Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1M NaNO3 or 35-45% CH3CN in 0.1% TFA)
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TSKgel G1000PW
TSKgel G1000PW is ideally suited for analysis of water-soluble polymers with molecular weights up to 1,000 Da.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
12μm |
Pore size (mean): |
< 100Å |
Exclusion limit: |
< 1,000 Da(polyethylene glycols and oxides) |
Max. % organic: |
20% |
Shipping solvent: |
water |
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Applications |
Polyethylene glycol and oxide calibration curves for TSK-GEL PW columns
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
05760 |
TSKgel G1000PW |
12μm |
Stainless Steel |
7.5 |
30 |
06763 |
Guard Column for P/N 05760 |
13μm |
Stainless Steel |
7.5 |
7.5 |
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TSKgel G2000PW
TSKgel G2000PW is ideally suited for analysis of water-soluble polymers with molecular weights up to 2,000 Da.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
12μm |
Pore size (mean): |
125Å |
Calibration range: |
up to 2,000 Da (polyethylene glycols and oxides) |
Max. % Organic |
20% |
Shipping solvent: |
water |
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Applications |
Polyethylene glycol and oxide calibration curves for TSK-GEL PW columns
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
05761 |
TSKgel G2000PW |
12μm |
Stainless Steel |
7.5 |
30 |
05105 |
TSKgel G2000PW |
12μm |
Stainless Steel |
7.5 |
60 |
06763 |
Guard Column for P/N 05761 and 05105 |
13μm |
Stainless Steel |
7.5 |
7.5 |
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TSKgel G2500PW
TSKgel G2500PW is ideally suited for the analysis of water-soluble polymers with molecular weights of less than 3,000 Da.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
12μm and 17μm |
Pore size (mean): |
<200Å |
Calibration range: |
up to 3000 Da (polyethylene glycols & oxides) |
Max. % organic: |
20% |
Shipping solvent: |
water |
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Applications |
Polyethylene glycol and oxide calibration curves for TSK-GEL PW columns
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
08028 |
TSKgel G2500PW |
12μm |
Stainless Steel |
7.5 |
30 |
08029 |
TSKgel G2500PW |
12μm |
Stainless Steel |
7.5 |
60 |
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Guard Column for P/N 08028, 08029 |
13μm |
Stainless Steel |
7.5 |
7.5 |
16248 |
TSKgel G2500PW |
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Stainless Steel |
21.5 |
30 |
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TSKgel G2500PW |
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Stainless Steel |
21.5 |
60 |
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Guard Column for P/N 16248, 08030 |
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Stainless Steel |
21.5 |
7.5 |
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TSKgel G3000PW
TSKgel G3000PW is ideally suited for the analysis of water-soluble polymers of less than 50,000 Da molecular weight.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
12μm and 17μm |
Pore size (mean): |
200Å |
Calibration range: |
up to 50,000 Da (polyethylene glycol and oxides) |
Max. % organic: |
20% |
Shipping solvent: |
water |
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Applications |
Polyethylene glycol and oxide calibration curves for TSK-GEL PW columns
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
05762 |
TSKgel G3000PW |
12µm |
Stainless Steel |
7.5 |
30 |
05106 |
TSKgel G3000PW |
12µm |
Stainless Steel |
7.5 |
60 |
06762 |
Guard Column for P/N 05762, 05106 |
13µm |
Stainless Steel |
7.5 |
7.5 |
16249 |
TSKgel G3000PW |
17µm |
Stainless Steel |
21.5 |
30 |
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Guard Column for P/N 16249 |
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Stainless Steel |
21.5 |
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TSKgel G4000PW
TSKgel G4000PW is ideally suited for water analysis of water-soluble polymers of less than 300,000 Da molecular weight.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
17μm |
Pore size (mean): |
500Å |
Calibration range: |
up to 300,000 Da (polyethylene glycols and oxides) |
Max. % organic: |
20% |
Shipping solvent: |
water |
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Applications |
Polyethylene glycol and oxide calibration curves for TSK-GEL PW columns
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
05763 |
TSKgel G4000PW |
17μm |
Stainless Steel |
7.5 |
30 |
05107 |
TSKgel G4000PW |
17μm |
Stainless Steel |
7.5 |
60 |
06762 |
Guard Column for P/N 05763 and 05107 |
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Stainless Steel |
7.5 |
7.5 |
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TSKgel G5000PW
TSKgel G5000PW is ideally suited for the analysis of water-soluble polymers with a molecular weight of less than 1,000,000 Da.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
17μm |
Pore size (mean): |
1,000Å |
Calibration range: |
up to 1,000,000 Da (polythylene glycols & oxides) |
Max. % organic: |
20% |
Shipping solvent: |
water |
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Applications |
Polyethylene glycol and oxide calibration curves for TSK-GEL PW columns
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
05764 |
TSKgel G5000PW |
17μm |
Stainless Steel |
7.5 |
30 |
05108 |
TSKgel G5000PW |
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Stainless Steel |
7.5 |
60 |
06762 |
Guard Column for P/N 05764 and 05108 |
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Stainless Steel |
7.5 |
7.5 |
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TSKgel G6000PW
TSKgel G6000PW It is ideally suited for the analysis of water-soluble polymers up to 8,000,000 Da molecular weight.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
17μm |
Pore size (mean): |
>1,000Å |
Calibration range: |
up to 8,000,000 Da (polythylene glycols and oxides) |
Max. % organic: |
20% |
Shipping solvent: |
water |
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Applications |
Polyethylene glycol and oxide calibration curves for TSK-GEL PW columns
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
05765 |
TSKgel G6000PW |
17μm |
Stainless Steel |
7.5 |
30 |
05109 |
TSKgel G6000PW |
17μm |
Stainless Steel |
7.5 |
60 |
06762 |
Guard Column for P/N 05765 and 05109 |
13μm |
Stainless Steel |
7.5 |
7.5 |
20024 |
TSKgel BioAssist G6PW |
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PEEK |
7.8 |
30 |
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TSKgel GMPW
TSKgel GMPW is a mixed bed scouting column for water-soluble linear polymers. The pore volume can be accessed by polymers ranging in molecular weight from 500 to 8,000,000 Da. By quickly categorizing the MW profile of an unknown sample, the column enables a fast selection of the best TSK-GEL PW column for routine analysis. Mixed bed columns are also used when a sample has a very broad MW distribution.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
17μm |
Pore size (range): |
< 100Å - 1000Å |
Calibration range |
500 - 8,000,000 Da (polythylene glycols and oxides) |
Max. % organic: |
20% |
Shipping solvent: |
water |
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Applications |
Effect of ionic strength on th elution of anionic polymers
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
08026 |
TSKgel GMPW |
17μm |
Stainless Steel |
7.5 |
30 |
08027 |
TSKgel GMPW |
17μm |
Stainless Steel |
7.5 |
60 |
06762 |
Guard Column for P/N 08026 and 08027 |
13μm |
Stainless Steel |
7.5 |
7.5 |
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TSK-GEL PWxl-type Columns for Analysis of Waters-soluble Polymers
TSK-GEL PWxl columns are composed of spherical, hydrophilic polymethacrylate beads. The main application area for PWxl columns is the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. See the table on the TSK-GEL PW page for typical samples and suitable eluents. Figure 1 shows the calibration curves for all PWxl-type columns. Figure 2 shows the benefits of PWxl columns over PW columns, namely the improved resolution due to the smaller particle sizes used in PWxl columns.
Figure 1: Polyethylene glycol and oxide calibration curves on TSK-GEL PWxl columns
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Figure 2: Faster analysis and higher resolution with TSK-GEL PWxl columns
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Although proteins can be analyzed on PWxl columns, pore volumes are much smaller than on SW-type columns. Figure 3 demonstrates the limited separation power of PWxl for protein samples.
Specialty Columns
TSKgel G-DNA-PW is available for the analysis of DNA fragments. Similarly, TSKgel G-Oligo-PW is the preferred column for the analysis of oligosaccharides. The mixed-mode column, TSKgel GMPWxl, has an extended linear calibration range, suitable for samples with a broad MW distribution and unknowns.
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Figure 3: Protein Calibration curves for TSK-GEL PWxl
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TSKgel G2500PWxl
TSK-GEL G2500PWxl columns are ideally suited for analysis of water-soluble linear polymers with molecular weights up to 3,000 Da. Because of the presence of cationic groups on the base bead of G2500PWxl, this column is not suited for separating anionic polymers. The smaller particle size of PWxl-type columns provide 1.7X higher resolution than their PW counterpart, making PWxl-type columns more suitable for analytical purposes.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
7μm |
Pore size (mean): |
< 200Å |
Calibration range: |
< 3,000Da
(polythylene glycols and oxides) |
Max. % organic: |
20% |
Shipping solvent: |
water |
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Applications |
Polyethylene glycol and oxide calibration curves on TSK-GEL PWxl columns
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
08020 |
TSKgel G2500PWxl |
7μm |
Stainless Steel |
7.8 |
30 |
08033 |
Guard Column for P/N 08020 |
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Stainless Steel |
6 |
4 |
08035 |
TSKgel Top-Off for PWxl, 1g |
10μm |
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TSKgel G3000PWxl
TSK-GEL G3000PWxl columns are ideally suited for analysis of water-soluble linear polymers with molecular weights up to 50,000 Da. The smaller particle size of PWxl-type columns provide 1.7X higher resolution than their PW counterpart, making PWxl-type columns more suitable for analytical purposes.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
7μm |
Pore size (mean): |
200Å |
Calibration range: |
< 50,000 Da (polyethylene glycols and oxides) |
Max. % organic: |
20% |
Shipping solvent: |
water |
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Applications |
Polyethylene glycol and oxide calibration curves on TSK-GEL PWxl columns
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
08021 |
TSKgel G3000PWxl |
7μm |
Stainless Steel |
7.8 |
30 |
08033 |
Guard Column for P/N 08021 |
12μm |
Stainless Steel |
6 |
4 |
08035 |
TSKgel Top-Off for PWxl, 1g |
10μm |
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TSKgel G4000PWxl
TSK-GEL G4000PWxl columns are ideally suited for analysis of water-soluble linear polymers with molecular weights up to 300,000 Da. The smaller particle size of PWxl-type columns provide 1.7X higher resolution than their PW counterpart, making PWxl-type columns more suitable for analytical purposes.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
10μm |
Pore size (mean): |
<500Å |
Calibration range: |
< 300,000 Da
(polythylene glycols and oxides) |
Max. % organic: |
20% |
Shipping solvent: |
water |
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Applications |
Polyethylene glycol and oxide calibration curves on TSK-GEL PWxl columns
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
08022 |
TSKgel G4000PWxl |
10μm |
Stainless Steel |
7.8 |
30 |
08033 |
Guard Column for P/N 08022 |
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Stainless Steel |
6 |
4 |
08035 |
TSKgel Top-Off for PWxl, 1g |
10μm |
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TSKgel G5000PWxl
TSK-GEL G5000PWxl columns are ideally suited for analysis of water-soluble linear polymers with molecular weights up to 1 million Da. The smaller particle size of PWxl-type columns provide 1.7X higher resolution than their PW counterpart, making PWxl-type columns more suitable for analytical purposes.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
10μm |
Pore size (mean): |
1000Å |
Calibration range: |
< 1,000,000 Da
(polythylene glycols and oxides) |
Max. % organic: |
20% |
Shipping solvent: |
water |
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Applications |
Polyethylene glycol and oxide calibration curves on TSK-GEL PWxl columns
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
08023 |
TSKgel G5000PWxl |
10μm |
Stainless Steel |
7.8 |
30 |
08033 |
Guard Column for P/N 08023 |
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Stainless Steel |
6 |
4 |
08035 |
TSKgel Top-Off for PWxl, 1g |
10μm |
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TSKgel G6000PWxl
TSKgel G6000PWxl columns are ideally suited for analysis of water-soluble linear polymers with molecular weights up to 8 million Da. The smaller particle size of PWxl-type columns provide 1.7X higher resolution than their PW counterpart, making PWxl-type columns more suitable for analytical purposes.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
13μm |
Pore size (mean): |
> 1000Å |
Calibration range: |
less than 8,000,000 Da (polythylene glycols and oxides) |
Max. % organic: |
20% |
Shpioing solvent: |
water |
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Applications |
Polyethylene glycol and oxide calibration curves on TSK-GEL PWxl columns
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
08024 |
TSKgel G6000PWxl |
13μm |
Stainless Steel |
7.8 |
30 |
08033 |
Guard Column for P/N 08024 |
12μm |
Stainless Steel |
6 |
4 |
08035 |
TSKgel Top-Off for PWxl, 1g |
10μm |
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TSKgel G-DNA-PW
TSKgel G-DNA-PW is a specialty column designed for the separation of large polynucleotides such as DNA and RNA fragments of 500-5000 base pairs. TSKgel G-DNA-PW is a smaller particle size version of TSKgel G6000PWxl.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
10μm |
Pore size (mean): |
>1,000Å |
Working range: |
500 - 5000 bp DNA |
Max. % organic: |
20% |
Shipping solvent: |
water |
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Applications |
Calibration curve for double-stranded DNA
Separation of large DNA fragments on a
TSKgel G-DNA-PW column
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
08032 |
TSKgel G-DNA-PW |
10μm |
Stainless Steel |
7.8 |
30 |
08033 |
Guard Column for P/N 08032 |
12μm |
Stainless Steel |
6 |
4 |
08035 |
TSKgel Top-Off for P/N 08032, 1g |
10μm |
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TSKgel GMPWxl
TSKgel GMPWxl is a mixed bed scouting column for aqueous water-soluble linear polymers. Its pore volume is accessible to polymers ranging from molecular weights of 500 up to 8,000,000 Da. By quickly categorizing the MW profile of an unknown sample, TSKgel GMPWxl allows the selection of the most appropriate TSK-GEL PWxl column for best results in a routine analysis. TSKgel GMPWxl is also used for the analysis of samples with broad MW distributions.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
13μm |
Pore size (range): |
100Å - 1000Å |
Calibration range: |
500-8,000,000 Da(polyethylene glycols and oxides) |
Max. % organic: |
20% |
Shipping solvent: |
water |
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Applications |
Separation of polyethyleneimine
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559s.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
08025 |
TSKgel GMPWxl |
13μm |
Stainless Steel |
7.8 |
30 |
08033 |
Guard Column for P/N 08025 |
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Stainless Steel |
6 |
4 |
08035 |
TSKgel Top-Off for P/N 08025, 1g |
10μm |
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TSKgel G-Oligo-PW
TSKgel G-Oligo-PW is designed for high resolution separations of aqueous nonionic and cationic oligomers, and oligosaccharides such as hydrolyzed cyclodextrins. Because of the presence of cationic groups on the gel matrix, this column is not suited for separating anionic polymers. The PEG and PEO calibration curve is identical to that of G2500PWxl.
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Product Attributes:
Base material: |
hydroxylated polymethacrylate |
Particle size (mean): |
7μm |
Pore size (mean): |
125Å |
Calibration range: |
up to 3,000 Da (polythylene glycols and oxides) |
Max. % organic: |
20% |
Shpiing solvent: |
water |
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Applications |
Calibration curve for oligosaccharides
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
08031 |
TSKgel G-Oligo-PW |
7μm |
Stainless Steel |
7.8 |
30 |
08034 |
Guard Column for P/N 08031 |
13μm |
Stainless Steel |
6 |
4 |
08035 |
TSKgel Top-Off for P/N 08031, 1g |
10μm |
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TSKgel PWxl-CP for Cationic Polymers
TSK-GEL PWxl-CP columns were specifically developed for the analysis of water-soluble cationic polymers. Composed of polymethacrylate beads, cationic groups are introduced on the surface of the TSK-GEL PWxl-CP packing material to prevent adsorption of cationic polymers and allow elution under low salt conditions. These columns show high theoretical plate numbers, linear calibration curves and high durability because the base resin is the same as that used in the TSK-GEL PWxl series columns.
Three columns are available within the TSK-GEL PWxl-CP series, each with a different particle size, separation range and exclusion limit, allowing polymers within a wide molecular mass range to be separated and characterized.
Product Attributes:
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G3000PWxl-CP |
G5000PWxl-CP |
G6000PWxl-CP |
Base material: |
polymethacrylate |
polymethacrylate |
polymethacrylate |
Particle size: |
7µm |
10µm |
13µm |
Pore size: |
200Å |
1,000Å |
>1,000Å |
Exclusion limit (Da):
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100,000 |
1,000,000 |
20,000,000 |
Separation range (Da)
(PEO,PEG): |
200~50,000 |
400~500,000 |
1,000~10,000,000 |
| Theoretical plates: |
16,000 |
10,000 |
7,000 |
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Applications |
Polyethylene glycol and oxide calibration curves on TSK-GEL PWxl-CP columns.
Analysis of a wide variety of cationic polymers
Various cationic polymers with different functional groups and molecular weights were injected on the TSK-GEL PWXL-CP columns (TSKgel G6000PWXL-CP, G5000PWXL-CP and G3000PWXL-CP, connected in series).
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Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle
size |
Housing material |
ID
(mm) |
Length
(cm) |
21873 |
TSKgel G3000PWxl-CP |
7μm |
Stainless Steel |
7.8 |
30 |
21874 |
TSKgel G5000PWxl-CP |
10μm |
Stainless Steel |
7.8 |
30 |
21875 |
TSKgel G6000PWxl-CP |
13μm |
Stainless Steel |
7.8 |
30 |
21876 |
Guard Column for P/N's 21873, 21874, 21875 |
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Stainless Steel |
6 |
4 |
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TSK-GEL SuperMultiporePW columns are packed with spherical, mono-disperse, polymethacrylate particles incorporating the proprietary multi-pore particle technology. Each particle each contains a wide range of pore sizes in order to provide near linear calibration curves. They belong to the semi-micro type of SEC columns (6 mm ID, 15 cm length) providing high theoretical plate numbers at half of the length of a conventional SEC column. The TSK-GEL SuperMultiporePW series comprises of three column types covering different molecular weight ranges (PW-N; PW-M, PW-H).
The main application area for SuperMultiporePW columns is the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. Figure 1 shows the near linear calibration curves for the three SuperMultiporePW-type columns. Figure 2 shows the benefits of SuperMultiporePW columns over a combination of different conventional SEC columns, namely the improved resolution and the smooth peak shapes without any inflection points.
Product Attributes:
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SuperMultiporePW-N |
SuperMultiporePW-M |
SuperMultiporePW-M
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Base material: |
polymethacrylate |
polymethacrylate |
polymethacrylate
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Particle size: |
4 µm |
5 µm |
8 µm (6-10 µm)
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Exclusion limit (Da): |
100,000 - 150,000 |
600,000 - 1,500,000 |
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Separation range (Da)
(PEO,PEG): |
300 ~ 50,000 |
600,000 ~ 1,500,000 |
1,000~10,000,000 |
Theoretical plates:
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> 16,000 |
> 12,000 |
> 7,000
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 Literature: |
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| Application |
Analysis of polyvinylpyrrolidone
MW calibration curve
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Ordering information
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle size
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Dimension
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22789 |
TSKgel SuperMultiporePW-N |
4 μm |
6 mm ID x 15 cm L
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22790 |
TSKgel SuperMultiporePW-M
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5 μm |
6 mm ID x 15 cm L
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22791 |
TSKgel SuperMultiporePW-H
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8 μm (6-10 μm) |
6 mm ID x 15 cm L
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22793 |
TSKgel SM(PW)-N Guard Column for P/N 22989
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5 μm |
4.6 mm ID x 3.5 cm L
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22795 |
TSKgel SM(PW)-H Guard Column for P/N 21873, 21874, 21875
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8 μm (6-10 μm) |
4.6 mm ID x 3.5 cm L
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TSKgel SuperOligoPW column packed with spherical, mono-disperse polymethacrylate particles. It belongs to the semi-micro type of SEC columns (6 mm ID, 15 cm length) providing high theoretical plate numbers at half of the length of a conventional SEC column. The main application area for the SuperOligoPW columns is the analysis of water-soluble oligomers or hydrolysates of water soluble polymers. Figure 1 shows the calibration curve for SuperOligoPW. Figure 2 shows the benefits of the SuperOligoPW semi-micro column over a conventional SEC column, namely the improved resolution and short analysis time.
Application
Polyethylene glycol and oxide calibration curves for TSK-GEL SuperOligoPW column
Analysis of PEG 200. Comparison between TSKgel SuperOligoPW and TSKgel G-Oligo-PW
Product Attributes:
| Product Attributes:
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Base material: |
polymethacrylate
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Particle size (mean): |
3 μm
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Exclusion limit (Da)
(PEO, PEG):
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4,000-8,000
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Separation range (Da)
(PEO, PEG):
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100-3,000
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Theoretical plates:
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> 16,0000
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Ordering information
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
P/N |
Description |
Particle size
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Dimension
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22792 |
TSKgel SuperOligoPW |
3 μm |
6 mm ID x 15 cm L
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22796 |
TSKgel SuperOligoPW |
3 μm |
4.6 mm ID x 3.5 cm L
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