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Molecular weight calibration standards
The TSK-GEL standards product line consists of bulk quantities polystyrene and polyethylene oxide standards of varying molecular weight. For premixed PStQuick calibration standards click here. The standards are primarily used to calibrate size exclusion columns for subsequent analysis of unknown samples. The available polystyrene standards range from 400 to 21,000,000 Da while the polyethylene oxides range from 18,000 to 1,000,000 Da. All standards are tested to certify quality and reliability. Testing is done by laser light scattering, except for the standards below 1,000Da, which are tested by size exclusion chromatography.
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559.
Polystyrene calibration standards
P/N |
Description |
Nominal MW (Da) |
Amount |
05202 |
A-300 |
400 |
10g |
05203 |
A-500 |
530 |
10g |
05204 |
A-1000 |
950 |
10g |
05205 |
A-2500 |
2800 |
5g |
05206 |
A-5000 |
6200 |
5g |
05207 |
F-1 |
10,000 |
5g |
05208 |
F-2 |
17,000 |
5g |
05209 |
F-4 |
44,000 |
5g |
05210 |
F-10 |
100,000 |
5g |
05211 |
F-20 |
190,000 |
5g |
05212 |
F-40 |
420,000 |
5g |
05213 |
F-80 |
800,000 |
5g |
05214 |
F-128 |
1,300,000 |
1g |
05215 |
F-288 |
2,900,000 |
1g |
05216 |
F-380 |
3,800,000 |
1g |
05217 |
F-450 |
4,500,000 |
1g |
05218 |
F-550 |
5,500,000 |
1g |
05219 |
F-700 |
6,800,000 |
1g |
05220 |
F-850 |
8,400,000 |
1g |
05221 |
F-2000 |
21,000,000 |
1g |
06476 |
Polystyrene Oligomer Kit, A-500 thru F-128 |
12x1g |
06477 |
High MW Kit F-10 thru F-2000 |
12x1g |
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Polyethylene oxide calibration standards
P/N |
Description |
Nominal MW (Da) |
Amount |
06211 |
SE-2 |
18,000 |
0.5g |
06212 |
SE-5 |
39,000 |
0.5g |
06213 |
SE-8 |
86,000 |
0.5g |
06214 |
SE-15 |
150,000 |
0.5g |
06215 |
SE-30 |
250,000 |
0.5g |
06216 |
SE-70 |
590,000 |
0.5g |
06217 |
SE-150 |
1,000,000 |
0.5g |
05773 |
Polyethylene oxide (PEO) Kit, SE-2 thru SE-150 |
7x0.2g |
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The molecular weights for the above listed polystyrene and polyethylene oxide calibration standards are determined by laser light scattering, except for polystyrene standards A-300, A-500 and A-1000 which are based on size exclusion chromatography. Results vary among individual lots.
Replacement Parts
Tosoh Bioscience offers a limited line of replacement parts for its columns and guard columns. With the introduction of TSK-GEL BioAssist series, the TSK-GEL column line consists of stainless steel, glass and PEEK columns.
The tubing, fittings and frits in the stainless steel TSK-GEL columns are manufactured from 316-grade stainless steel. The top and bottom end-fittings are engraved with the pore size of the frit. If the top frit plugs, the entire end-fitting can be removed and replaced. Order the appropriate replacement fitting according to the ID of the column. Stainless steel end-fittings will connect to the 10-32 PEEK or stainless steel nuts and ferrules on a standard HPLC system.
The end-fittings on the glass columns contain Pyrex® frits with a nominal 10μm pore size. The materials in the glass column fittings that come into contact with the sample and the mobile phase are chlorotrifluoroethylene and tetrafluoroethylene. The end-fittings will connect to 1/4-28 and also to M6 nuts and ferrules.
Tosoh Bioscience recommends that each column be protected by a guard column and filter. Guard columns are listed with corresponding columns in each section of this website. We recommend installing a pre-column filter in front of the (guard) column to avoid clogging the top frit, particularly when working with columns that contain smaller than 5μm particles. In addition, when suspecting debris from the breakdown of pump seals, we have found it beneficial to use a pre-injector membrane filter to prevent any particulates from reaching the injector and/or (guard) column.
Ordering information:
To order or get a price, please contact Søren Jensen, sorenj@md-scientific.dk, Mobile 2421 2559s.
Guard column products
P/N |
Description |
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19018 |
TSKgel Guard Cartridge Holder, Replaces P/N 14100 |
For 4.6mm ID guard columns
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18206 |
TSKgel Super-guard Filter Holder |
For Super-ODS, -Octyl and -Phenyl guard filters (PN 18207)
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19308 |
TSKgel Cartridge Holder |
For all 2mm ID guard columns |
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03432 |
TSKgel Guardgel Holder |
For all 7.5mm ID stainless steel columns
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08809 |
Guardgel Holder, Glass |
For all 5mm and 8mm columns
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16106 |
Guardgel Holder |
For all 21.5mm ID stainless steel columns
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Fittings
P/N |
Description |
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18255 |
Endfitting with fixed 1µm Frit |
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16103 |
Endfitting with fixed 1µm Frit |
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07619 |
Endfitting with fixed 2µm Frit |
For all 4.6mm ID stainless steel columns
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08095 |
Endfitting with fixed 2µm Frit |
For all 7.8mm ID stainless steel columns
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05748 |
Endfitting with fixed 10µm Frit |
For all 7.5mm ID stainless steel columns
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06191 |
Endfitting with fixed 10µm Frit |
For all 21.5mm ID stainless steel columns
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16104 |
Endfitting with fixed 10µm Pyrex Frit |
For all 5mm ID glass columns |
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08600 |
Endfitting with fixed 10µm Pyrex Frit |
For all 8mm ID glass columns
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16105 |
Endfitting with fixed 10µm Pyrex Frit |
For all 20mm ID glass columns |
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08092 |
Endfitting with fixed 2µm Frit |
For all 6mm ID stainless steel columns
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03400 |
Plug, 1/4-28, 10 pk |
For glass column fitting |
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02342 |
Male nut and ferrule 10-32, 1/16", stainless steel, 2 pk |
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16566 |
One-piece fingertight fitting, 1/16", 10-32, PEEK |
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18545 |
Endfitting with fixed 2µm Frit |
For all 2mm stainless steel columns |
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19834 |
Endfitting with 5µm Frit |
For 4.6mm BioAssist columns |
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Pre-injector filters
P/N |
Description |
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14594 |
Pre-injector membrane filter holder, stainless steel |
For collecting debris generated by pump seals
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03409 |
13mm Nylon membrane filters 0.45µm |
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Pre-column filters
P/N |
Description |
03410 |
Pre-column filter with 0.5µm stainless steel frit |
03411 |
Replacement 0.5µm stainless steel frits, pk 10 |
03430 |
Replacement 2µm stainless steel frits, pk 10, for P/N 03432 |
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Tubing
P/N |
Description |
02353 |
Teflon Tubing, 1/16" x 0.01" x 150cm |
02354 |
PEEK Tubing, 1/16" x 0.01" x 150cm |
02359 |
Tefzel Tubing, 1/16" x 0.03" x 150cm |
02374 |
Stainless Steel Tubing, 1/16" x 0.01" x 10cm, 5pk |
02375 |
Stainless Steel Tubing, 1/16" x 0.02" x 10cm, 5pk |
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For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.
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