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The differential viscometer detector provides a direct and sensitive measurement of intrinsic viscosity and allows for the determination of molecular size, conformation and structure.
Viscotek invented and patented the first differential viscometer detector and that experience is packed into the 270 giving high sensitivity, excellent baseline stability and wide applications flexibility. In the measuring flow-path, the use of inert capillaries means the analysis of proteins and other biomolecules can be completed with confidence.
For polymers and polysaccharides, the viscosity information shows the complete Mark-Houwink relationship of molecular weight to intrinsic viscosity at all points to determine structure and measure the branching properties such as number of branches vs. molecular weight.
For proteins, the determined intrinsic viscosity gives a clear measurement of the protein density.
When used together with the molecular weight data from the light scattering detector, the intrinsic viscosity gives a direct measurement of protein or polymer size (RH) with sensitivity down to < 1 nm radius.
More information
Application communication: Universal calibration to study star-branched polystyrene
It is shown in this application communication that the number of branches of a star polymer can be assessed by merely adding a viscometer detector to a conventional chromatography system.
Application communication: Determination of polymer structure by GPC
It is advantageous to use light scattering to determine molecular weights provided the signals are sufficiently intensive strong. Triple detection combines these detection capabilities into a single system to give molecular weight and structure without limitations.
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