GPC/SEC kolonner fra Viscotek/Malvern

Selection of the correct column is essential for successful GPC/SEC experiments as obtaining the best chromatographic separation requires a careful match of sample, solvent and column. In most cases, the solubility of the sample will clearly dictate when you need to use an aqueous or organic eluent.

Below are details on the four ranges of columns we offer.

Aqueous GPC/SEC columns
Rugged methacrylate based columns designed for the separation of water-soluble polymers and oligomers.

Organic GPC/SEC columns
A wide selection of SDV based columns for all organic solvent soluble polymers.

Protein SEC columns
Macro-porous silica columns designed specifically for the separation of proteins in advanced SEC experiments.

GPC/SEC columns for specialized applications
Specialised columns for samples and solvents where chemical activity or charges present a challenge to conventional GPC/SEC columns.

Viscotek A-columns for aqueous GPC/SEC

Viscotek A-Columns are designed for the separation of water-soluble polymers and oligomers by size exclusion chromatography (SEC). The packing material is a porous poly hydroxymethacrylate polymer. There are 8 types of columns for different molecular weight ranges.


Column size:	Analytical columns	300mm L×7.8mm ID
	Guard column	50mm L×6.0mm ID (AGuard) 50mm L×8.0mm ID (A7Guard)

Packing material	Porous poly hydroxymethacrylate polymer

Connecting screws	Internally-threaded type, No.10 32 UNF

Column material	Stainless steel type SUS-316

Shipping solvent	0.02% sodium azide aqueous solution (water with antibacterial)

Max pressure	(A2000-A3000) 5.0MPa (A4000-A6000M) 3.0MPa (A7000) 0.5MPa

Max flow rate	(A2000-A6000M) 1.2mL/min (A7000) 1.5mL/min

Temperature Range	(A2000-A6000M) 4 - 70 ℃ (A7000) 4- 60℃

pH range	3 -10

Usable salt concentration	< 0.5 M

Organic solvents	refer to Table 2

Table 1: Specifications

PN	Grade	Particle Size (μm)	Theoretical Plate Number (per column)	Exclusion Limit MW Pullulan	Max. pore size (A)	Max pressure (MPa)
CLM3015	A2000, Aq GPC/SEC Col	8	> 12,000	4,000	100	5.0
CLM3016	A2500, Aq GPC/SEC Col	6	> 16,000	10,000	200	5.0
CLM3017	A3000, Aq GPC/SEC Col	6	> 16,000	100,000	800	5.0
CLM3018	A4000, Aq GPC/SEC Col	10	> 16,000	1,000,000	2,000	3.0
CLM3019	A5000, Aq GPC/SEC Col	13	> 12,000	(4,000,000)	7,000	3.0
CLM3020	A6000, Aq GPC/SEC Col	13	> 12,000	(20,000,000)	15,000	3.0
CLM3021	A6000M, Aq GPC/SEC Col	13	> 12,000	(20,000,000)	15,000	3.0
CLM3022	A7000, Aq GPC/SEC Col	35	> 1,500	(500,000,000)	30,000	0.5
CLM3023	AGuard	10	guard column
CLM3024	A7Guard	35	guard column

Table 2: Organic solvent compatibility ranges

Column type	Methanol	Acetonitrile	DMF	DMSO
A2000	0 %	0 %	0 %	0%
A2500 - see caution b)	0 - 100 %	0 - 75 %	0 - 100 %	100%
A3000	0 - 100 %	0 - 75 %	0 - 100 %	0%
A4000, A5000, A6000, A6000M	0 - 75 %	0 - 75 %	0 - 100 %	0%
A7000	0 - 30%	0 - 30%	0%	0%

Note: Protein samples
Urea or 6 M guanidine aqueous solutions, which are commonly used as protein modifiers, can be used as the eluent. An eluent containing surfactant, such as SDS or Brij-35, is recommended for samples such as membrane proteins when their solubility in water is poor.

Viscotek T-Columns for Organic GPC/SEC

Viscotek T-columns are designed for the separation of organic polymers and oligomer by gel permeation chromatography/size exclusion chromatography (GPC/SEC). The packing material is a porous styrene divinylbenzene copolymer. There are 14 types of columns for different molecular weight ranges.


Column size:	Analytical columns	300mm L×7.8mm ID
	Guard column	10mmL×4.6mmID

Packing material	Porous styrene divinylbenzene copolymer

Connecting screws	Internally-threaded type, No.10 32 UNF

Column material	Stainless steel type SUS-316

In-column solvent	THF

Max pressure	3.5MPa per column

Max flow rate	(analysis) 2.0mL/min (solvent exchange) 0.3mL/min

Temperature Range	10 – 60 ℃

Organic solvents	refer to Table 2

Table 1: Specifications

PN	Grade	Particle Size (μm)	Theoretical Plate Number (per column)	Exclusion Limit MW polystyrene	Max. pore size (A)
CLM3000	T1000, Org GPC/SEC Col	6	> 18,000	1,500	50
CLM3001	T2000, Org GPC/SEC Col	6	> 18,000	5,000	150
CLM3002	T2500, Org GPC/SEC Col	6	> 18,000	20,000	300
CLM3003	T3000, Org GPC/SEC Col	6	> 18,000	70,000	500
CLM3004	T4000, Org GPC/SEC Col	7	> 18,000	400,000	1,500
CLM3005	T5000, Org GPC/SEC Col	10	> 11,000	4,000,000	5,000
CLM3006	T6000, Org GPC/SEC Col	10	> 11,000	(20,000,000)	10,000
CLM3007	T7000, Org GPC/SEC Col	18	> 6,000	(200,000,000)	20,000
CLM3008	TGuard	8	guard column
CLM3009	T6000M, General Mixed Org	10	> 13,000	(20,000,000)	10,000
CLM3010	LT3000L, Mixed, Ultra-Low Org	6	> 18,000	70,000	500
CLM3011	LT4000L, Mixed, Low Org	7	> 18,000	400,000	1,500
CLM3012	LT5000L, Mixed, Medium Org	10	> 11,000	4,000,000	5,000
CLM3013	LT6000L, Mixed, High Org	10	> 11,000	(20,000,000)	10,000
CLM3014	LT7000L, Mixed, Ultra-High Org	18	> 6,000	(200,000,000)	20,000

Table 2: Column/Solvent compatibility and exchanges

Solvent	Column
	T1000	T2000 T2500	T3000	T4000 T5000 T6000 T7000 T6000M
	T1000	LT3000L LT4000L	T3000	LT5000L LT6000L LT7000L
THF	O	O	O	O
Chloroform	O	O	O	O
Carbon tetrachloride	×	O	O	O
Benzene	O	O	O	O
Toluene	O	O	O	O
p-Xylene	×	O	O	O
o-Dichlorobenzene(ODCB)	×	×	O	O
Trichlorobenzene(TCB)	×	×	O	O
Dioxane	×	O	O	O
Diethyl ether	×	×	O	O
Ethyl acetate	×	×	O	O
Acetone	×	×	O	O
Methyl ethyl ketone	×	×	O	O
Dimethylformamide(DMF)	×	×	O	O
Dimethylacetamide(DMAc)	×	×	O	O
Hexafluoroisopropanol(HFIP)	×	×	×	O
m-Cresol	×	×	O	O
o-Chlorophenol	×	×	O	O
Quinolin	×	×	O	O
N-Methylpyrrolidone(NMP)	×	×	O	O
Dimethylsulfoxide(DMSO)	×	×	×	△
30% m-Cresol/Chloroform	×	O	O	O
30% o-Chlorophenol/Chloroform	×	O	O	O
30% HFIP/Chloroform	×	O	O	O
Hexane	×	×	×	×
Acetonitrile	×	×	×	×
Methanol	×	×	×	×
Water	×	×	×	×

O Can be exchanged
△Can be exchanged but may cause the column performance to deteriorate.
× Cannot be used/exchanged

Viscotek P-columns for protein SEC

Viscotek P-Columns are designed for the separation of biopolymers such as proteins, enzymes and polysaccharides by Size Exclusion Chromatography (SEC). The packing material is a spherical porous silica gel coated with hydrophilic hydroxyl polymer. There are three types of columns for different molecular weight ranges.


Column size:	Analytical columns	300mm L×7.8mm ID
	Guard column	50mm L×6.0mm ID

Packing material	Porous silica gel coated with chemically-bonded hydroxyl polymer

Connecting screws	Internally-threaded type, No.10 32 UNF

Column material	Stainless steel type SUS-316

Shipping solvent	H2O

Max flow rate	(analysis) 1.5mL/min (solvent exchange) 0.5mL/min

Temperature Range	10 - 45 ℃

pH range	3.0 - 7.5

Organic solvents	0 – 100% Methanol or Acetonitrile

Table 1: Specifications

PN	Grade	Particle Size (μm)	Theoretical Plate Number (per column)	Exclusion Limit MW		Max. pore size (A)	Max. Pressure (MPa)
PN	Grade	Particle Size (μm)	Theoretical Plate Number (per column)	Protein	Pullulan	Max. pore size (A)	Max. Pressure (MPa)
CLM3025	P2500, Protein SEC Col	5	>21,000	150,000	60,000	400	5.0
CLM3026	P3000, Protein SEC Col	5	> 21,000	700,000	170,000	1,000	5.0
CLM3027	P4000, Protein SEC Col	7	> 16,000	(1,000,000)*	500,000	1,500	5.0
CLM3028	PGuard	7	guard column

* Estimated values

Viscotek I-Columns for specialized GPC/SEC applications

The Viscotek I-Series speciality columns are designed for the separation of difficult to elute polar organic polymers. The packing is a fluorinated, highly crossed-linked di-vinyl-benzene (DVB) gel that resists degradation during quick solvent changes, ideal for scouting different solvents. Quick solvent change in one hour (e.g. from THF to DMF). This inert column is compatible from toluene to formic acid and can support up to 20% water.


Analytical column size:	300mm L x 7.8 mm ID

FIPA column size:	100mm L x 7.8 mm ID

Guard column size:	40mm L x 7.8 mm ID

Semi-prep column size:	300mm L x 22 mm ID

Packing material:	Fluorinated DVB gel

Mechanical connection:	10-32 Valco length

Column material:	Stainless Steel SUS-316

Shipping solvent:	Dichoromethane (CH2Cl2)

Temperature range:	4 - 70 °C

pH range:	2 - 12

Organic solvent:	Organic, up to 20% water

Table 1: Specifications

PN	Particle size (μm)	Exclusion Limit MW (g/mol)	Max Pressure (MPa)	Mac Pressure (psi)	Dimensions L (mm) x ID (mm)
I-OLIGO-3078	5	>10 k	17	2500	300 x 7.8
I-MBLMW-3078	5	>20 k	17	2500	300 x 7.8
I-MBMMW-3078	5	>200 k	10	1500	300 x 7.8
I-MBHMW-3078	5	>10 M	10	1500	300 x 7.8
I-MBLMW-1078	5	>20 k	17	2500	100 x 7.8
I-Guard-0478	5	>20 k	17	2500	40 x 7.8

More information

Application communication: Structural differences in modified starches
The Viscotek triple detection system provides a convenient and rapid way to characterize starches and modified starches. The instrument allows determination of molecular weight and molecular size in a single run using normal conditions and sample concentrations. The IV and size data allow differentiation between molecules of differing structures. The technique is equally applicable to other polysaccharides and all other synthetic or natural polymers such as proteins and DNA.

Application Note: Characterization of chitosan from various sources
This note proves that the use of conventional viscometric measurements of chitosan to estimate the molecular weight using Ubbelhode-type viscometer are flawed as the M-H curve exhibits a curvature. However, the Viscotek triple detector was used to study successfully the molecular weight and conformation of chitosan.

Application communication: Determination of polymer structure by GPC
It is advantageous to use light scattering to determine molecular weights provided the signals are sufficiently intensive strong. Triple detection combines these detection capabilities into a single system to give molecular weight and structure without limitations.

Application communication: Characterization of branched copolymers by triple detection GPC
Polymers can be loosely grouped into two structural categories – linear and branched. A linear polymer is composed of a single main chain of repeating units, linked in a regular end to tail fashion.

Application communication: Characterization of modified phenolic resins
The triple detection chromatography technique was shown to be able to detect the modification of the phenolic resins. The high molecular weight is easily revealed in the light scattering signal while the viscometer gives information on the partial molar volume occupied by the polymer.

Application communication: Protein and polymer molecular size by GPC/SEC
The main goal of most Gel Permeation/Size Exclusion Chromatography (GPC/SEC) experiments is to determine the molecular weight distribution (MWD) of the sample or compare molecular weights (MW) of several samples.

Application Note: SELS – Solvent Enhanced Light Scattering
Viscotek has been a strong advocate of good chromatography as a prerequisite for GPC (Gel Permeation Chromatography) data accuracy. Our recent work in application development has been driven by extremely difficult samples from industrial, biopharmaceutical and academic sources.

Application Note: Solvent Enhanced Light Scattering (SELS) of Fluoro-Polyester
Traditionally in GPC, the sample dissolution solvent and the mobile phase are one and the same. However, a closer look at the demands of sample solvent and mobile phase suggests that this should not always be the case.

For flere detaljer og pris, kontakt venligst MD Scientific via e-mail: info@md-scientific.dk eller telefon: 7027 8565.

GPC/SEC kolonner fra Viscotek/Malvern

Viscotek A-columns for aqueous GPC/SEC

Table 1: Specifications

Table 2: Organic solvent compatibility ranges

Viscotek T-Columns for Organic GPC/SEC

Table 1: Specifications

Table 2: Column/Solvent compatibility and exchanges

Viscotek P-columns for protein SEC

Table 1: Specifications

Viscotek I-Columns for specialized GPC/SEC applications

Table 1: Specifications

More information